FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066436
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56380.5286768263637012No Hit
GTCTTCAGGCTGGAGGTTGCTGATGGTGAGAGTGAAGTCAGTCCCGAATCCACTGCCACTGAAGCGTGGCGGGAC52390.4912624855124921No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43050.40368104602620314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38710.3629847454512038No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37650.35304509600201045No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33720.31619337681773685No Hit
GCCTGAAGACTGTGCAACTTACTATTGTCAACAGGCTAATGTCTTTCCTC32740.30700388959112407No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30610.2870308204149147No Hit
GTTTATGAATAGGTGGCTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTCATCTATGCTGGAGC29110.2729652787415279No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29100.2728715084637053No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28690.26902692707297954No Hit
GTGCAGCCACAGTTCGATTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG28550.2677141431834634No Hit
AGTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTC28520.2674328323499957No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27300.255992858455641No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26660.24999156067499598No Hit
CCTGAAGACTGTGCAACTTACTATTGTCAACAGGCTAATGTCTTTCCTCG25530.23939551928104452No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25270.23695749205765748No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24310.22795554538668988No Hit
GCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24030.22532997760765766No Hit
GGCTAATGTCTTTCCTCGCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAACTGTGGCTGCACCAT23960.2246735856628996No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22240.20854509787741599No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21980.20610707065402892No Hit
CTATAGGGGACAGAGTCACCATGACTTGTCGGGCGAGTCAGTTTATGAAT21930.20563821926491604No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21880.20516936787580314No Hit
GACTTCACTCTCACCATCAGCAACCTCCAGCCTGAAGACTGTGCAACTTACTATTGTCAACAGGCTAATGTCTTT21520.2017936378741903No Hit
ATAGGGGACAGAGTCACCATGACTTGTCGGGCGAGTCAGTTTATGAATAGGTGGCTAGCCTGGTATCAGCAGAAA21450.20113724592943227No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21160.19841790787257746No Hit
GTAATAAACGGCCGTGTCTTCGGCGGTCACAGACGTCAGATTCAGGAAGAACCGATTCTTGGACGTGTCCACGGA20530.19251038036975496No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20390.19119759648023885No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20260.18997858286854533No Hit
GAGTCACCATGACTTGTCGGGCGAGTCAGTTTATGAATAGGTGGCTAGCCTGGTATCAGCAGAAACCAGGAAAAG19820.18585269064435184No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19700.18472744731048088No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATGTCCACCTTGGTCC19180.17985139286370677No Hit
GTCCGTGGACACGTCCAAGAATCGGTTCTTCCTGAATCTGACGTCTGTGACCGCCGAAGACACGGCCGTTTATTA19120.1792887711967713No Hit
GAGTGAAGTCAGTCCCGAATCCACTGCCACTGAAGCGTGGCGGGACCCCA18730.17563173036169072No Hit
CATCTATGCTGGAGCCACTTTGCAAAGTGGGGTCCCGCCACGCTTCAGTGGCAGTGGATTCGGGACTGACTTCA18270.17131829758185207No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18240.17103698674838436No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17910.16794256758023923No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17890.16775502702459408No Hit
GTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCTAGCCACCTATTC17830.16719240535765859No Hit
ACTTACTATTGTCAACAGGCTAATGTCTTTCCTCGCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAA17750.16644224313507797No Hit
CAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT17530.1643792970229812No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17520.16428552674515864No Hit
GGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17440.16353536452257803No Hit
CTTCAGTGGCAGTGGATTCGGGACTGACTTCACTCTCACCATCAGCAACCTCCAGCCTGAAGACTGTGCAACTTA17440.16353536452257803No Hit
GACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCC17190.16119110757701352No Hit
CCCCTAAACTCCTCATCTATGCTGGAGCCACTTTGCAAAGTGGGGTCCCG16870.158190458686691No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16260.15247047173951367No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16170.15162653923911046No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15750.14768818757056212No Hit
GTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15560.14590655229193314No Hit
ATTCATAAACTGACTCGCCCGACAAGTCATGGTGACTCTGTCCCCTATAG15250.14299967367943317No Hit
ACATTAGCCTGTTGACAATAGTAAGTTGCACAGTCTTCAGGCTGGAGGTTGCTGATGGTGAGAGTGAAGTCAGTC15170.14224951145685255No Hit
GACCCAGTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGG15090.1414993492342719No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14970.14037410590040095No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC14650.13737345701007841No Hit
ATAGTAAGTTGCACAGTCTTCAGGCTGGAGGTTGCTGATGGTGAGAGTGAAGTCAGTCCCGAATCCACTGCCA14540.13634198395403005No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14290.13399772700846557No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14140.1325911728411269No Hit
CCTTGGTCCCTCCGCCGAAAGTGCGAGGAAAGACATTAGCCTGTTGACAATAGTAAGTTGCACAGTCTTCAGG14120.13240363228548174No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14080.13202855117419143No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14010.13137215922943338No Hit
GTCTCCATCTTCCGTGTCGGCTTCTATAGGGGACAGAGTCACCATGACTT13940.13071576728467532No Hit
GTGGGGTCCCGCCACGCTTCAGTGGCAGTGGATTCGGGACTGACTTCACT13910.13043445645120758No Hit
CACCATGACTTGTCGGGCGAGTCAGTTTATGAATAGGTGGCTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCC13790.12930921311733662No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13720.12865282117257856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13720.12865282117257856No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13700.1284652806169334No Hit
CTCCTCATCTATGCTGGAGCCACTTTGCAAAGTGGGGTCCCGCCACGCTTCAGTGGCAGTGGATTCGGGACTGA13680.12827774006128825No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13580.12734003728306248No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13530.12687118589394958No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13400.12565217228225603No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13120.12302660450322382No Hit
ATGCTGGAGCCACTTTGCAAAGTGGGGTCCCGCCACGCTTCAGTGGCAGTGGATTCGGGACTGACTTCACTCTCA13070.12255775311411092No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13040.12227644228064319No Hit
CCTCCAGCCTGAAGACTGTGCAACTTACTATTGTCAACAGGCTAATGTCTTTCCTCGCACTTTCGGCGGAGGGAC12650.1186194014455626No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12610.11824432033427229No Hit
GTTTATTACTGTGCGAGAGAAGGAGATTACGGTGACTACTACTTTCACCA12440.11665022561128845No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12370.11599383366653038No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12350.11580629311088522No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12290.11524367144394976No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12220.1145872794991917No Hit
GTCCACGGACAGGGCGACTCGACTCTTGAGGGAGGGGTTGTAGATGGTACTTCCCCCGACATGGACACGCCCAAT11970.11224302255362721No Hit
GGACCAAGGTGGACATCAATCGAACTGTGGCTGCACCATCTGTCTTCATC11660.10933614394112727No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11530.10811713032943375No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11480.10764827894032085No Hit
ATTGTCAACAGGCTAATGTCTTTCCTCGCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAACTGTGGCTG11460.1074607383846757No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11260.1055853328282241No Hit
GACCAAGGTGGACATCAATCGAACTGTGGCTGCACCATCTGTCTTCATCT11250.10549156255040151No Hit
GTGCAACTTACTATTGTCAACAGGCTAATGTCTTTCCTCGCACTTTCGGC11120.10427254893870799No Hit
TGGTGACTCTGTCCCCTATAGAAGCCGACACGGAAGATGGAGACTGGGTC11060.10370992727177253No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10880.10202206227096611No Hit
GCATAGATGAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGC10790.10117812977056288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT209.265606E-567.8309317
GTGGTAT2400.050.830241
GTATCAA19900.038.8251081
TCTTTCG350.001474674738.7132765
CTGTTAA400.001752583337.37597767
TATCAAC22350.034.5545232
CCCTTAT400.002834853333.886831
ATCAACG22850.033.796823
TCAACGC22950.033.6479844
CAACGCA22900.033.5782745
AACGCAG23350.032.9373326
ACGCAGA24500.031.3927657
CGCAGAG24550.031.340598
CGTTTAT5200.030.90705767
AGAGTAC24950.030.85116211
CGGGATA1101.2714736E-930.83224117
CCGTTTA5150.030.73325366
CAGAGTA25400.030.43237310
GCAGAGT25800.030.0877239
ACGGGTA1251.5279511E-1029.84140616
Produced by FastQC (version 0.11.5)