FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567055
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC74740.47694560816308296No Hit
GTCTAATAGTTATCCGCGCACTTTCGGCGGAGGGACCAAGGTGGACACCAAGCGAACTGTGGCTGCACCAT70490.4498246711187546No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52750.3366186891972522No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46660.2977559817619675No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42730.27267709174215327No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36130.2305598718615492No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34450.21983912498285No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31800.2029284230610923No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31720.20241791130496378No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29790.1901018151883629No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28950.18474144174901327No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28800.18378423220627227No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27950.1783600447974066No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27100.17293585738854092No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26270.1676392979187074No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25440.16234273844887384No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCA25370.16189604066226138No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25310.16151315684516498No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24080.15366403859468877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23910.15257920111291562No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCACTTATTTAGCCTGGTATCAGCAAAAA23600.15060096805791756No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23510.15002664233227297No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23470.1497713864542087No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23030.14696357179550174No Hit
GATCTATGGTTCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA22050.14070980278292722No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21780.1389868256059934No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21550.13751910430712386No Hit
GTGTCCACCTTGGTCCCTCCGCCGAAAGTGCGCGGATAACTATTAGACTG21310.13598756903873827No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT21030.1342007778922884No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20460.1305633816298726No Hit
GTGCAGCCACAGTTCGCTTGGTGTCCACCTTGGTCCCTCCGCCGAAAGTG19970.12743649712358532No Hit
GTATTATTGTGCGTCCAGTCGTTTCTACTACTATTACCCCATCGACGTCGGGGGCCAAGGGACCACGGTCTCCGT19660.12545826406858726No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19550.12475631040391051No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19450.12411817070874984No Hit
CAATAATACACAGCCGAGTCCTCAACTCCCAGGTTGTTCATTTCCAGAGA19380.12367147292213738No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19310.12322477513552492No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18930.12079984429391438No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTGAGCTCCTGAT18900.12060840238536617No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18770.11977882078165732No Hit
CCATAGATCAGGAGCTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC18570.11850254139133597No Hit
GGTGGACACCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC18430.11760914581811105No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17600.11231258634827751No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC17580.11218495840924537No Hit
GTGGATGAACCATAGATCAGGAGCTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAAGTGCTAATG17350.11071723711037584No Hit
ACTTATTACTGTCAACAGTCTAATAGTTATCCGCGCACTTTCGGCGGAGGGACCAAGGTGGACACCAAGCGAA17290.11033435329327944No Hit
GTCTCCGACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG17200.10976002756763482No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16970.10829230626876529No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16930.10803705039070102No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16850.10752653863457248No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16790.10714365481747608No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16580.1058035614576387No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16410.10471872397586557No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTCTAATAGTTAT16390.10459109603683343No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16300.10401677031118882No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16080.10261286298183536No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15990.10203853725619075No Hit
ATAATACACAGCCGAGTCCTCAACTCCCAGGTTGTTCATTTCCAGAGACA15910.10152802550006221No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG15840.10108132771344976No Hit
GGCTAAATAAGTGCTAATGCCCTGACTGGCCCGGCAAGTGATGGTGACTC15750.10050700198780516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT601.07320375E-1051.2390539
TCGTATG1500.050.2908240
CTTATAC4800.049.6580661
TTATACA5150.047.568892
CGTATGC1700.046.55126641
CGAGACT5300.045.6329223
ACGAGAC5500.044.56310722
CACGAGA5750.043.19608721
ATCGCGT950.042.85239437
TATACAC5850.041.8768843
CGTTTGC503.9152183E-641.28894841
ATGCCGT2000.040.04130644
ATCTCCG6200.039.51539210
TACACAT6350.039.1008385
TCCGAGC6300.038.8881613
GCATCGG1150.038.1353135
ACACATC6600.038.122566
GAGACTC6500.037.73013324
CCCACGA6600.037.6269519
GCATCGC1350.037.4834335
Produced by FastQC (version 0.11.5)