FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249182
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55650.4454915296570075No Hit
GTATTACTGCCAGCAGTATAGTTACTCACTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG54600.4370860290974414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48710.38993517357758917No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC37820.30275812491694565No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37320.2987555056028665No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36690.29371220526712677No Hit
CAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGT36460.2918710003826504No Hit
GTCCCAGACCCACTGCCCATGAACCTGTCTGGGACGCCAGAGGCCCTGAT34010.27225816574366263No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33680.26961643699637045No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGATT33450.267775232111894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30310.24263878281947704No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30180.24159810179781646No Hit
GTGTATTACTGCCAGCAGTATAGTTACTCACTCTCTTTCGGCGGAGGGAC29570.23671490623463995No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29290.23447343941875565No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28830.23079102964980283No Hit
CTCCTGCAGGGCCACTCAGACTGTTAAGAGCAAGTACTTAACCTGGTACC28340.22686846272200528No Hit
CATTTATAGTACATCCATCAGGGCCTCTGGCGTCCCAGACAGGTTCATGGGCAGTGGGTCTGGGACAGACTTCA27870.22310600056677088No Hit
GTATAGTTACTCACTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT27210.21782254307218643No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCAGACTGTTAAGAG26050.20853646626352285No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25710.20581468512994905No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25550.2045338469494437No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25410.20341311354150157No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC25240.20205222297471465No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24450.19572808445846962No Hit
GTTAAGAGCAAGTACTTAACCTGGTACCAACACAAACCTGGCCAGGCTCC24230.1939669319602748No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24180.19356667002886688No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23750.19012441741875885No Hit
GTACTTAACCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATAGTACATCCATCAGGGCCT23300.18652206003608762No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATAGTACATCCATCAGGGCCTCTGGCGTCCCAGACAGGTT22380.17915724049818202No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG22200.17771629754511353No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21750.1741139401624423No Hit
GTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGA21020.16827011596388677No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20880.1671493825559446No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC19850.1589039867689416No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19800.1585037248375337No Hit
GTAGTACACGGCAGTGTCCTCAGGCATTAGGTTGTTGATTTCCAGATATG18920.1514591148447544No Hit
GTCCCAGACAGGTTCATGGGCAGTGGGTCTGGGACAGACTTCACTCTCAC18900.15129901007219124No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCCATGAACCTGTCTGGGACGCCA18740.15001817189168593No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCAGA18470.14785675746208318No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18370.14705623359926737No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18240.1460155525776068No Hit
GACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGATTTTGCAGTGTATTACTGCCAGCAGTATAGTTACTCA18210.14577539541876205No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17990.14401424292056722No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17990.14401424292056722No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17910.14337382383031455No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17460.13977146644764332No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17180.13752999963175902No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16940.13560874236100104No Hit
CTGCTACTCTGGCTCCCAGGTTCAGATGGCGCCGGAGAAGTTGTGTTGAC16910.1353685852021563No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16650.13328722315883515No Hit
CTATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGT15880.12712318941515327No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15850.12688303225630854No Hit
GTCTGGGACGCCAGAGGCCCTGATGGATGTACTATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGTA15810.1265628227111822No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTTCAGAT15780.12632266555233745No Hit
GCAGTATAGTTACTCACTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15450.12368093680504523No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15360.12296046532851099No Hit
GGTTTGTGTTGGTACCAGGTTAAGTACTTGCTCTTAACAGTCTGAGTGGCCCTGCAGGAGAGGGTGGCTCTTTCC14900.11927805555955817No Hit
CTATACTGCTGGCAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCT14670.11743685067508178No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14580.11671637919854753No Hit
CTCCCAGGCTCCTCATTTATAGTACATCCATCAGGGCCTCTGGCGTCCCA14460.11575575056316854No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14390.11519538385919746No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14140.11319407420215788No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14050.11247360272562364No Hit
GTAACTATACTGCTGGCAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGT13970.11183318363537098No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13890.11119276454511834No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13780.11031218829602092No Hit
GTGTACTACTGTGCGAGACCCCTGAGTTACGATGTTTTGCCTGGTCTCCGTACATATTACTACCACTACTATGG13640.10919145488807877No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13610.10895129772923401No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13610.10895129772923401No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13440.10759040716244711No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13390.10719014523103919No Hit
GGTTCAGATGGCGCCGGAGAAGTTGTGTTGACGCAGTCTCCAGGCACCCT13290.10638962136822337No Hit
GTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATAGTACATCCATCAGGGCCTCTGGCGTCCCAGA13230.10590930705053386No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13170.10542899273284438No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13160.1053489403465628No Hit
CTGTTAAGAGCAAGTACTTAACCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATAGTACAT13010.10414815455233904No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG12890.10318752591696005No Hit
GCCCATGAACCTGTCTGGGACGCCAGAGGCCCTGATGGATGTACTATAAA12860.10294736875811532No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12850.10286731637183372No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12830.10270721159927057No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12830.10270721159927057No Hit
GCCTGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGTTAAGTACTTGCTC12730.10190668773645474No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12700.10166653057760999No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12500.10006548285197833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)