FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249182
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54540.43660571477975185No Hit
GTATTACTGCCAGCAGTATAGTTACTCACTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG48930.391696326075784No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45860.36712024348733807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37760.3022778105992561No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC36890.29531325299275846No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35590.28490644277615274No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGATT32480.2600101506425805No Hit
CAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGT32340.2588894172346383No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31700.2537660645126171No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30840.24688155929240094No Hit
GTCCCAGACCCACTGCCCATGAACCTGTCTGGGACGCCAGAGGCCCTGAT30830.24680150690611935No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29540.2364747490757952No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28570.22870966760648168No Hit
CTCCTGCAGGGCCACTCAGACTGTTAAGAGCAAGTACTTAACCTGGTACC28490.228069248516229No Hit
GTGTATTACTGCCAGCAGTATAGTTACTCACTCTCTTTCGGCGGAGGGAC28240.22606793885918947No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCAGACTGTTAAGAG25620.20509421365341476No Hit
CATTTATAGTACATCCATCAGGGCCTCTGGCGTCCCAGACAGGTTCATGGGCAGTGGGTCTGGGACAGACTTCA25520.20429368979059895No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC24760.19820970843319866No Hit
GTATAGTTACTCACTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT24680.19756928934294604No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24610.19700892263897493No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23820.1906847841227299No Hit
GTACTTAACCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATAGTACATCCATCAGGGCCT23620.18908373639709825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23560.18860342207940875No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23460.18780289821659293No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23280.18636195526352445No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23240.18604174571839813No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22960.1838002789025138No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22310.17859687379421094No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22290.17843676902164776No Hit
GTTAAGAGCAAGTACTTAACCTGGTACCAACACAAACCTGGCCAGGCTCC22110.17699582606857928No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCAGA21410.17139215902886848No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATAGTACATCCATCAGGGCCTCTGGCGTCCCAGACAGGTT20950.1677097492599157No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20760.16618875392056562No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG20580.16474781096749713No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20410.16338692040071023No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19900.1593042487003495No Hit
GACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGATTTTGCAGTGTATTACTGCCAGCAGTATAGTTACTCA19780.15834362006497052No Hit
GTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGA19330.1547412626822993No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18970.15185937677616232No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCCATGAACCTGTCTGGGACGCCA18580.14873733371118059No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC18480.14793680984836477No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18140.14521502871479094No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17900.143293771444033No Hit
GTCCCAGACAGGTTCATGGGCAGTGGGTCTGGGACAGACTTCACTCTCAC17820.14265335235378032No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17760.1421730380360908No Hit
GTAGTACACGGCAGTGTCCTCAGGCATTAGGTTGTTGATTTCCAGATATG17700.14169272371840133No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17140.1372097900866327No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17010.13616910906497212No Hit
CTGCTACTCTGGCTCCCAGGTTCAGATGGCGCCGGAGAAGTTGTGTTGAC16640.13320717077255356No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16030.12832397520937702No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15800.12648277032490063No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15660.12536203691695846No Hit
CTATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGT15640.1252019321443953No Hit
GGTTTGTGTTGGTACCAGGTTAAGTACTTGCTCTTAACAGTCTGAGTGGCCCTGCAGGAGAGGGTGGCTCTTTCC15520.1242413035090163No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15520.1242413035090163No Hit
GTCTGGGACGCCAGAGGCCCTGATGGATGTACTATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGTA15410.1233607272599189No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15210.12175967953428724No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15210.12175967953428724No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTTCAGAT15040.12039878896750032No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14970.11983842226352927No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14580.11671637919854753No Hit
GCAGTATAGTTACTCACTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14420.11543554101804221No Hit
CTCCCAGGCTCCTCATTTATAGTACATCCATCAGGGCCTCTGGCGTCCCA14180.11351428374728421No Hit
CTGTTAAGAGCAAGTACTTAACCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATAGTACAT14050.11247360272562364No Hit
GTGTACTACTGTGCGAGACCCCTGAGTTACGATGTTTTGCCTGGTCTCCGTACATATTACTACCACTACTATGG13940.11159302647652625No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13910.11135286931768149No Hit
CTATACTGCTGGCAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCT13750.11007203113717616No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13680.10951166443320509No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13590.10879119295667085No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13110.10494867841515487No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12920.1034276830758048No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12850.10286731637183372No Hit
GCCTGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGTTAAGTACTTGCTC12820.10262715921298898No Hit
GTAACTATACTGCTGGCAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGT12740.10198674012273633No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12740.10198674012273633No Hit
GTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATAGTACATCCATCAGGGCCTCTGGCGTCCCAGA12600.10086600671479418No Hit
GTTCATGGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGAC12550.10046574478338624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT755.752645E-754.53424570
GTGGTAT2900.048.6296731
GTACGTA806.7411747E-945.05337569
CGATCCG307.1734306E-444.8204217
CGGTACC404.7767237E-543.46333745
GGTATCA9400.042.1506461
TGGTATC3550.040.663362
AATGGTA6250.038.1739770
TTAACGG451.0799081E-437.8463935
AGAGTCG451.1693302E-437.3383811
GTATCAA25050.036.861671
TGGTACG854.8357833E-836.03611467
GGTACGT905.63723E-835.4131968
TAACGGC501.9895229E-434.1116336
ATACGTA1600.031.50425711
TACGTAC1600.031.50425712
CGCGGTA553.2155312E-431.43246743
ATCAACG29450.031.1200623
TCAACGC29550.031.0147514
CAACGCA29700.030.9736255
Produced by FastQC (version 0.11.5)