FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234041
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTTCCAGCAGAA76070.6164300861964878No Hit
GTATTATAATACTCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT53780.4358039967877891No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA51070.41384362432042365No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48980.39690739610758474No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT47290.38321255128476284No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT43170.3498263023675875No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41320.3348349041887587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36190.29326416221179036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31640.2563934261503467No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29250.23702616039499497No Hit
TTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC28930.23443305368298137No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28780.23321753491172495No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26230.21255371580036642No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25690.20817784822384344No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24880.201614046859059No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24740.200479562672553No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24430.19796749054528984No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24290.19683300635878384No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24200.19610369509603004No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24160.19577955675702832No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23980.19432093423152066No Hit
GTATTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT23610.19132265459575493No Hit
GTTTATTACTGTCAGCAGTATTATAATACTCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA23310.18889161705324214No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23070.18694678701923192No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA22290.18062608940869876No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21620.1751967722304202No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21210.17187435425565278No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20490.16603986415362212No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTA19860.1609346853143453No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA19860.1609346853143453No Hit
ATTATAATACTCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAA19750.16004330488209065No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA19180.15542433355131638No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18780.15218295016129935No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT18700.15153467348329594No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG18590.15064329305104124No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAA18570.1504812238815404No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18550.15031915471203955No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18190.1474019096610242No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18100.1466725983982704No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAATACT17950.145457079627014No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17890.14497087211851145No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17750.14383638793200548No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17660.14310707666925168No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA17510.1418915578979953No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17410.14108121205049104No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17230.13962258952498338No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17100.13856913992322784No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG16750.13573292945696294No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16620.1346794798552074No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16210.13135706188043994No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15910.1289260243379272No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC15890.12876395516842634No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15890.12876395516842634No Hit
ATTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15440.12511739885465717No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15240.12349670715964865No Hit
GTCGTGTCCCTTGGCCGAAGGTGAGGGGAGTATTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGC15080.12220015380364185No Hit
CCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15020.1217139462951393No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14690.11903980499837527No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14580.11814842456612058No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14440.11701394037961461No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14420.11685187121011377No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA14300.11587945619310866No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14240.1153932486846061No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG14190.11498807576085397No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14140.11458290283710186No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14060.11393462615909844No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGAGGGGAGTATT13880.11247600363359078No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13860.11231393446408994No Hit
ATAATACTCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA13680.11085531193858227No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13600.11020703526057887No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13560.10988289692157717No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13500.10939668941307461No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13220.10712772104006268No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13170.10672254811631057No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12910.1046156489127995No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12760.10340013014154312No Hit
TTATAATACTCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT12700.10291392263304054No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12570.10186047303128501No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12430.10072598884477905No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12410.10056391967527821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTA257.815128E-570.2028769
GGTATCA9050.047.8046881
TAGCTCG306.497241E-445.73020636
GACAGGT1950.045.4207271
GTGGTAT2250.043.9067041
CGTATGC2450.042.24012441
TCGTATG2400.041.63153540
ACGAGAC2750.040.88859622
GGGCGTA504.1927524E-640.878663
TATCTCG1850.040.786436
GACCGGA2750.039.6865526
AGCCTAT2250.039.5231932
GTATCAA23450.039.3678631
TGGTATC2700.039.1122932
TGCCGTC2400.038.9937345
GAGCCTA2650.038.6964931
CTATCTC2050.038.44891735
GCCTATC2150.038.20625333
ATGCCGT2650.037.88258444
ATCTCGT2000.037.75823237
Produced by FastQC (version 0.11.5)