FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805502
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31740.3940399899689883No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28340.3518302871997835No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26790.3325876285844107No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20260.25152017003061444No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19780.24556115316907964No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19200.23836067446139178No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19160.23786408972293055No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18680.23190507286139575No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17830.22135264716909453No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17030.21142095239986988No Hit
GTAGGAGACAGAGTCACCATCACCTGCCGGGCCAGTCAGGACATTGGAACTCATTTAGCCTGGTTTCAGCAAAAG16970.21067607529217805No Hit
AGTCAGGACATTGGAACTCATTTAGCCTGGTTTCAGCAAAAGCCAGGGAA16870.209434613446025No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16780.20831729778448718No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16220.20136511144602992No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16100.19987535723064626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16030.19900633393833905No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16010.19875804156910845No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15600.19366804799988083No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15420.19143341667680527No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15300.18994366246142158No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCGGGCCAGTCAGGACATTGGAA15200.1887022006152685No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14960.1857226921845011No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14770.18336391467681024No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14510.18013611387681222No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13380.16610759501528238No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT13280.16486613316912932No Hit
CATTTAGCCTGGTTTCAGCAAAAGCCAGGGAAAGCCCCTAAGCTCCTAAT13270.164741986984514No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13220.16412125606143746No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13170.1635005251383609No Hit
GTCCTGACTGGCCCGGCAGGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAA13130.16300394039989968No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12940.16064516289220884No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12940.16064516289220884No Hit
GTGCAGCCACAGTTCCTTTGAGATCCACCTTGGTCCCAGGGCCGAAAGTG12830.15927955486144044No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12740.1581622391999027No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12480.15493443839990464No Hit
GTGGATGCACCAAACATTAGGAGCTTAGGGGCTTTCCCTGGCTTTTGCTG12100.15021688338452296No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGAGATCCACCTTGGTCC11850.14711322876914024No Hit
GGCTAAATGAGTTCCAATGTCCTGACTGGCCCGGCAGGTGATGGTGACTC11330.1406576271691442No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11290.14016104243068297No Hit
CAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT11270.13991275006145235No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG10950.1359400721537625No Hit
GAATTCACTCTCACAATCAGCAGCCTGCGCCCTGACGATTTTGCAACTTATTTCTGTCAACAGCTTGATAAGTTC10890.13519519504607067No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10830.1344503179383788No Hit
GCCTTAGACCTGACGACACGGCCATCTATTATTGTGCGAGAGGCGCAGGG10710.1329605637229951No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10470.1299810552922277No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC10430.12948447055376647No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10200.12662910830761437No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10150.12600837738453782No Hit
GTCCCAGACCCAGAGCCGCTGAACGTTGATGGGACCCAACGTTGCAAAGT9950.12352545369223168No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9910.12302886895377045No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9890.12278057658453982No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9820.12191155329223267No Hit
CATTAGGAGCTTAGGGGCTTTCCCTGGCTTTTGCTGAAACCAGGCTAAAT9790.12153911473838674No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9630.11955277578454182No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9510.11806302156915811No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9500.11793887538454281No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9500.11793887538454281No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9420.11694570590762034No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9380.1164491211691591No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT9220.11446278221531418No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9190.11409034366146825No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9180.11396619747685295No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9080.11272473563069987No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9010.1118557123383927No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8940.11098668904608554No Hit
GCTCTGGGTCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCGCCCT8890.110365958123009No Hit
GTCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCGCCCTGACGATT8860.10999351956916309No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8800.10924864246147123No Hit
GTGCATCCACTTTGCAACGTTGGGTCCCATCAACGTTCAGCGGCTCTGGG8440.10477937981532012No Hit
GGACCAAGGTGGATCTCAAAGGAACTGTGGCTGCACCATCTGTCTTCATC8370.10391035652301299No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8340.10353791796916705No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8330.10341377178455174No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8300.10304133323070581No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8260.10254474849224458No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8250.10242060230762928No Hit
GCTTAGGGGCTTTCCCTGGCTTTTGCTGAAACCAGGCTAAATGAGTTCCA8240.10229645612301397No Hit
CAATAATAGATGGCCGTGTCGTCAGGTCTAAGGCTGTTCATTTGGAGAAA8230.10217230993839867No Hit
ACCCAGAGCCGCTGAACGTTGATGGGACCCAACGTTGCAAAGTGGATGCA8190.10167572519993744No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8150.1011791404614762No Hit
CCTTGGTCCCAGGGCCGAAAGTGGGAGGGAACTTATCAAGCTGTTGACAG8140.10105499427686089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTAAC150.002608874367.24323
CGCCAGT200.00612770754.2005357
ACGCTAC200.00814801250.432411
GGTATCA4300.049.2595521
TCGTATG1250.046.7082540
CGTATGC1300.045.0488941
ATGGTTA4500.043.49108570
ACGAGAC1350.042.35671622
CGCATAA350.001034764541.60371859
GTATGCC1450.040.49167342
TATACAC1700.039.554833
CGAGACT1450.039.4380123
ATGCCGT1500.039.30233844
CTTATAC1550.039.044441
ATGTTAG350.001522694738.460525
CCGTCTT1550.038.3166447
ATCTCGT1250.038.24735337
CACGAGA1500.038.11631421
CCGCATA400.0020323436.26432858
ACTAGGC1500.035.92771527
Produced by FastQC (version 0.11.5)