FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805502
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28120.34909907113824673No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27660.34338834664594253No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26170.3248905651382616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21700.26939722061521887No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20320.2522650471383063No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20160.2502787081844614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19820.2460577379075409No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19230.2387331130152377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16940.21030363673833213No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16690.20719998212294943No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16490.20471705843064322No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16220.20136511144602992No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15580.1934197556306502No Hit
GTAGGAGACAGAGTCACCATCACCTGCCGGGCCAGTCAGGACATTGGAACTCATTTAGCCTGGTTTCAGCAAAAG15570.1932956094460349No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15500.19242658615372774No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15330.1903161010152675No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15280.18969537009219095No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15060.18696415403065417No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14880.18472952270757861No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCGGGCCAGTCAGGACATTGGAA14590.18112928335373468No Hit
AGTCAGGACATTGGAACTCATTTAGCCTGGTTTCAGCAAAAGCCAGGGAA14520.18026026006142754No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14440.17926709058450505No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14250.1769083130768142No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14140.17554270504604583No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13760.1708251500306641No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT13620.1690871034460498No Hit
CATTTAGCCTGGTTTCAGCAAAAGCCAGGGAAAGCCCCTAAGCTCCTAAT13500.1675973492306661No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13470.16722491067682016No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13100.16263150184605377No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12970.16101760144605476No Hit
GTGCAGCCACAGTTCCTTTGAGATCCACCTTGGTCCCAGGGCCGAAAGTG12810.1590312624922098No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12460.15468614603067404No Hit
GTGGATGCACCAAACATTAGGAGCTTAGGGGCTTTCCCTGGCTTTTGCTG12340.15319639181529035No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12180.1512100528614454No Hit
GTCCTGACTGGCCCGGCAGGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAA12140.15071346812298417No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11740.14574762073837186No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11630.14438201270760345No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11580.1437612817845269No Hit
GAATTCACTCTCACAATCAGCAGCCTGCGCCCTGACGATTTTGCAACTTATTTCTGTCAACAGCTTGATAAGTTC11500.14276811230760444No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10820.1343261717537635No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10720.1330847099076104No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10650.13221568661530325No Hit
GTCCCAGACCCAGAGCCGCTGAACGTTGATGGGACCCAACGTTGCAAAGT10500.13035349384607361No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG10240.1271256930460756No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10160.12613252356915314No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGAGATCCACCTTGGTCC10080.1251393540922307No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC9950.12352545369223168No Hit
GGCTAAATGAGTTCCAATGTCCTGACTGGCCCGGCAGGTGATGGTGACTC9910.12302886895377045No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9910.12302886895377045No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9800.12166326092300206No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9800.12166326092300206No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9780.12141496855377143No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9740.1209183838153102No Hit
CATTAGGAGCTTAGGGGCTTTCCCTGGCTTTTGCTGAAACCAGGCTAAAT9690.12029765289223367No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9660.11992521433838774No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9650.11980106815377242No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9390.1165732673537744No Hit
CAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT9240.1147110745845448No Hit
GCTCTGGGTCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCGCCCT9090.11284888181531517No Hit
GTCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCGCCCTGACGATT8940.11098668904608554No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8860.10999351956916309No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8860.10999351956916309No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8780.10900035009224061No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8750.10862791153839468No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8630.107138157323011No Hit
GTGCATCCACTTTGCAACGTTGGGTCCCATCAACGTTCAGCGGCTCTGGG8520.1057725492922426No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8480.10527596455378137No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8430.10465523363070482No Hit
GCCTTAGACCTGACGACACGGCCATCTATTATTGTGCGAGAGGCGCAGGG8410.10440694126147422No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8220.10204816375378334No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8130.10093084809224558No Hit
GCTTAGGGGCTTTCCCTGGCTTTTGCTGAAACCAGGCTAAATGAGTTCCA8110.10068255572301496No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8100.10055840953839966No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8090.10043426335378436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAT200.0081515750.42682319
TTTAACG452.057217E-645.33434
TAACGGC405.351637E-542.63198536
ATTAGAG504.640924E-640.2763523
CACTTAG350.001539886338.372727
GTATCAA11200.038.065841
GCACCGC451.17050724E-437.3299910
ATGGTTA4700.037.04903870
GAATATA400.001865266736.90349662
ACGGGAT558.832454E-636.67177216
TATTAGA558.927034E-636.6148642
GGTATCA4450.035.4558221
CGCGGTA501.8305544E-434.59658443
GTGGTAT1750.034.529021
TATCAAC12100.034.3957822
ACAATGC502.168337E-433.61370518
TCAACGC12900.032.262714
GTTATGT450.0036262932.22467858
CAACGCA13100.031.770155
CGCAGAG13250.031.6854348
Produced by FastQC (version 0.11.5)