FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760057
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27670.3640516434951589No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26570.34957904472954004No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACGACAGAGGTGCTTCTAAGTGTATATGAA24860.327080732102987No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22840.3005037780061232No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACGACAGAGGTGCTT22280.29313590954362634No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC21940.2886625608342532No Hit
TCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATCACTGCAGTTCATAT20620.27129544231551056No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC20340.2676115080842621No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG19840.26103305409988986No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19410.25537558367332974No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17810.23432453092333866No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17370.22853549141709106No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17080.22471998810615518No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16380.2155101525280341No Hit
GTGTATATGAACTGCAGTGATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGC15930.20958954394209908No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTACTGACATT15830.20827385314522462No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15810.20801071498584975No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15690.20643188602960041No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15460.20340579719678917No Hit
CTCCTGCACTGGAACCAGTACTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC14650.19274870174210618No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14400.18945947474992006No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTACTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA14310.18827535303273307No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14110.18564397143898417No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATAA14020.1844598497217972No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14010.18432828064210974No Hit
CATATACACTTAGAAGCACCTCTGTCGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTG13950.18353886616398507No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13660.17972336285304918No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13560.17840767205617472No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13390.17617099770148817No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13310.1751184450639886No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12960.17051352727492805No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12760.16788214568117918No Hit
TGTCAGTACTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGAC12750.16775057660149173No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12590.16564547132649263No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12380.16288252065305628No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12250.1611721226171195No Hit
GTACTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCA12100.15919858642180784No Hit
CCCCTGAGGGCCGAGAAGCGACATCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA11990.15775132654524596No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11930.1569619120671213No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11890.1564356357483715No Hit
CCTTGGTCCCTCCGCCGAAAACGACAGAGGTGCTTCTAAGTGTATATGAACTGCAGTGATAATCAGCCTCGTCCT11640.1531464087561854No Hit
GATTATCACTGCAGTTCATATACACTTAGAAGCACCTCTGTCGTTTTCGG11550.1519622870389984No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11540.15183071795931094No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11400.1499887508436867No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11120.14630481661243827No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT10540.1386738099905665No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10320.13577929023744273No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10310.13564772115775528No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10230.13459516852025571No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT10170.13380575404213105No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACGACAGAGGTG10040.13209535600619426No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10040.13209535600619426No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9770.12854299085463325No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC9650.12696416189838394No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACGACAGAGGTGCTTC9140.12025413883432426No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8890.11696491184213814No Hit
GACCATCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATCACTGCAGTTCATATACACTTAGAAGCACCT8880.1168333427624507No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8840.11630706644370092No Hit
GTTCATATACACTTAGAAGCACCTCTGTCGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG8820.11604392828432604No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC8770.11538608288588882No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8730.11485980656713904No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8710.11459666840776415No Hit
GTACTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTT8710.11459666840776415No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8670.11407039208901437No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8580.11288627037182737No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG8520.1120968558937027No Hit
GTTATAACCACCAATGTCAGTACTGGTTCCAGTGCAGGAGATGGTGATCG8470.11143901049526549No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8420.11078116509682827No Hit
GTGATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG8410.11064959601714082No Hit
CCTCTGTCGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC8360.1099917506187036No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGATGTCGCTTCT8350.10986018153901615No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8250.10854449074214172No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8150.10722879994526727No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8150.10722879994526727No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8120.10683409270620493No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8120.10683409270620493No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8100.10657095454683005No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGAACTGTGG8070.1061762473077677No Hit
GTATATGAACTGCAGTGATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGA7980.1049921255905807No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7910.1040711420327686No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGAACTGTGGCTGCACCAT7830.10301858939526903No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTA7810.10275545123589415No Hit
CTTAGAAGCACCTCTGTCGTTTTCGGCGGAGGGACCAAGGTGACCGTCCT7790.10249231307651926No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7770.10222917491714437No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7690.1011766222796448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)