FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760057
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35390.46562297301386607No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27120.35681534411234944No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC24780.32602817946548746No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24310.31984443272017754No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23460.3086610609467448No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACGACAGAGGTGCTTCTAAGTGTATATGAA23120.3041877122373717No Hit
TCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATCACTGCAGTTCATAT20470.2693219061201989No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG20080.26419071201238853No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACGACAGAGGTGCTT17600.2315615802499023No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC16710.21985193215771975No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16700.2197203630780323No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16640.21893094859990764No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16550.21774682688272062No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15970.21011582026084888No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15630.20564247155147575No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14880.19577479057491742No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14560.1915645800249192No Hit
GTGTATATGAACTGCAGTGATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGC14530.19116987278585684No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14510.19090673462648194No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTACTGACATT14480.19051202738741962No Hit
CTCCTGCACTGGAACCAGTACTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC14110.18564397143898417No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTACTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA13750.18090748457023617No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13120.17261863254992718No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12760.16788214568117918No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12730.16748743844211683No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATAA12610.1659086094858675No Hit
CATATACACTTAGAAGCACCTCTGTCGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTG12550.16511919500774283No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12410.16327722789211863No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12210.16064584629836973No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12100.15919858642180784No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11660.1534095469155603No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11650.15327797783587283No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11650.15327797783587283No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11210.14748893832962529No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11180.14709423109056294No Hit
GTACTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTT11170.14696266201087552No Hit
GATTATCACTGCAGTTCATATACACTTAGAAGCACCTCTGTCGTTTTCGG11130.14643638569212572No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11050.14538383305462615No Hit
CCCCTGAGGGCCGAGAAGCGACATCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA10920.1436734350186894No Hit
TGTCAGTACTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGAC10800.14209460606244004No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10760.1415683297436903No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10590.13933165538900372No Hit
CCTTGGTCCCTCCGCCGAAAACGACAGAGGTGCTTCTAAGTGTATATGAACTGCAGTGATAATCAGCCTCGTCCT10430.13722655011400461No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10160.13367418496244363No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10160.13367418496244363No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10050.13222692508588174No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10040.13209535600619426No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9870.1298586816515077No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9600.12630631649994672No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC9280.1220961059499485No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACGACAGAGGTG9250.12170139871088616No Hit
GACCATCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATCACTGCAGTTCATATACACTTAGAAGCACCT9190.12091198423276148No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9130.12012256975463684No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9100.11972786251557449No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTA8910.11722805000151304No Hit
GTTCATATACACTTAGAAGCACCTCTGTCGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG8890.11696491184213814No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC8740.11499137564682647No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8650.11380725392963949No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8620.11341254669057715No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8520.1120968558937027No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8460.11130744141557804No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8410.11064959601714082No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT8240.10841292166245425No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8160.1073603690249547No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8160.1073603690249547No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGAACTGTGG8090.1064393854671426No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8090.1064393854671426No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGATGTCGCTTCT8010.10538683282964303No Hit
GTTATAACCACCAATGTCAGTACTGGTTCCAGTGCAGGAGATGGTGATCG8000.1052552637499556No Hit
CCTCTGTCGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC8000.1052552637499556No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7900.10393957295308115No Hit
GTGATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG7790.10249231307651926No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT7740.10183446767808205No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG7720.10157132951870715No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG7700.10130819135933226No Hit
GTATATGAACTGCAGTGATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGA7670.10091348412026993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)