FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004687111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567075
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATTACTGTGCGCTGTTTATCAGAGGTGGCATTTCGGTGTTCGGCGGAGGGACCAAACTAACCGTCGTAGGT88050.5618748304963068No Hit
GTAATAATGAGATTCATCTTCTGCCTGGGCCCCCGTGATGGTGAGGGCAG83400.532201713383214No Hit
GTTTATCAGAGGTGGCATTTCGGTGTTCGGCGGAGGGACCAAACTAACCGTCGTAGGTCAGCCCAAGGCTGCCCC71780.4580508271780227No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC67880.43316369669607385No Hit
GCCTTGGGCTGACCTACGACGGTTAGTTTGGTCCCTCCGCCGAACACCGAAATGCCACCTCTGATAAACAGCGCA66800.4262718759472265No Hit
CTGATAAACAGCGCACAGTAATAATGAGATTCATCTTCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC58280.37190306781743054No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG54920.3504618477099054No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53050.33852878770958633No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT49660.3168961281368154No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTTG48050.3066222101686263No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACTCTCACTTGTGGCTTGAGCTCTGG47000.2999218288850246No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46520.29685879744109245No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTTAGTTTGGTCCCTC42590.27178022749389785No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT40540.25869853070210425No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39690.25327441252014105No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG37280.2378954421453983No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG37040.23636392642343218No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG36490.23285420289392658No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35590.22711101893655378No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT35260.2250051848188504No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT34780.22194215337491824No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34560.220538263963116No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32080.20471260150279982No Hit
CTACTAGTTACTCTCCAAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACA32020.2043297225723083No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31480.2008838121978846No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31010.19788459390903435No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC30340.1936091125185457No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30110.19214140995166154No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT29970.19124802578051467No Hit
GTCTCTACTAGTTACTCTCCAAGCTGGTACCAGCAGACCCCAGGCCAGGC29830.19035464160936777No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29220.18646203914937065No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29120.18582390759855144No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG28230.18014453679626055No Hit
TTATTACTGTGCGCTGTTTATCAGAGGTGGCATTTCGGTGTTCGGCGGAG27420.174975671234625No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27360.1745927923041335No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27350.17452897914905158No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTTAGTTTGGTCCCTCCGCCGAACAC26970.1721040792559386No Hit
CAGTAATAATGAGATTCATCTTCTGCCTGGGCCCCCGTGATGGTGAGGGC26940.17191263979069285No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACT26560.16948773989757987No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26420.168594355726433No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC25920.165403697972337No Hit
CCCCTGGAGGGACAGTCACTCTCACTTGTGGCTTGAGCTCTGGCTCAGTC25560.16310642438938788No Hit
GGCCTGGGGTCTGCTGGTACCAGCTTGGAGAGTAACTAGTAGAGACTGAG25480.1625959191487325No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24730.1578099325175885No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC24550.15666129572611392No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24330.1552574063143117No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGAGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC23900.15251344064578912No Hit
GACCTACGACGGTTAGTTTGGTCCCTCCGCCGAACACCGAAATGCCACCT23630.1507904854585773No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23340.1489399039612016No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23270.14849321187562814No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC22300.14230333583268193No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT21960.14013368855989664No Hit
CACCATCACGGGGGCCCAGGCAGAAGATGAATCTCATTATTACTGTGCGC21890.13968699647432317No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21870.13955937016415934No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21540.13745353604645597No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA21490.1371344702710464No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21100.1346457572228515No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG20830.13292280203563964No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC19670.1255204760461369No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGAGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG19660.12545666289105498No Hit
CTCCAAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATC19250.12284032353269626No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTTAGTTTGGTCCCTCCGCCGAACACCGAAATGCCAC19180.12239363144712283No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19120.1220107525166313No Hit
AACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGAGTGACTGTC19080.12175549989630362No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG18960.12098974203532058No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18770.1197772920887641No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18700.11933060000319066No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18640.11894772107269914No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18640.11894772107269914No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTCTCCAAGCTGGTACCAGCAGACCCCAGGCCAGG18230.11633138171434042No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17730.11314072396024441No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17700.11294928449499865No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT17560.11205590032385176No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17560.11205590032385176No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17130.1093119346553292No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17100.10912049519008343No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC16840.10746135315795351No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16800.10720610053762583No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG16790.10714228738254392No Hit
CTGTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTTGGAGAGTAACTAGTAGAGACTGAG16660.10631271636647895No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16500.10529170588516822No Hit
CCATCACGGGGGCCCAGGCAGAAGATGAATCTCATTATTACTGTGCGCTG16390.1045897611792671No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA16290.10395162962844791No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16250.10369637700812023No Hit
GCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATC16150.10305824545730102No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC15950.10178198235566262No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTCTCCAAGCTGGTACCAGCAGACCCCAGGCCAGGCTC15910.10152672973533494No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15840.1010800376497615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)