FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567075
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATTACTGTGCGCTGTTTATCAGAGGTGGCATTTCGGTGTTCGGCGGAGGGACCAAACTAACCGTCGTAGGT79640.508207967072412No Hit
GTAATAATGAGATTCATCTTCTGCCTGGGCCCCCGTGATGGTGAGGGCAG72090.46002903498556225No Hit
GTTTATCAGAGGTGGCATTTCGGTGTTCGGCGGAGGGACCAAACTAACCGTCGTAGGTCAGCCCAAGGCTGCCCC67510.43080260995804287No Hit
GCCTTGGGCTGACCTACGACGGTTAGTTTGGTCCCTCCGCCGAACACCGAAATGCCACCTCTGATAAACAGCGCA60150.3838361278177496No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC59890.3821769857856197No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56380.3597785683518657No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG52600.33565719573089997No Hit
CTGATAAACAGCGCACAGTAATAATGAGATTCATCTTCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC51240.32697860663975875No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACTCTCACTTGTGGCTTGAGCTCTGG50460.322001180543369No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT47800.30502688129157823No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTTG43630.27841679562241756No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43080.27490707209291193No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT38740.24721216278735864No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG37140.2370020579742514No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTTAGTTTGGTCCCTC36840.23508766332179376No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35750.22813202941786448No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG35180.22449467957819505No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34330.21907056139623182No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33790.21562465102180814No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33790.21562465102180814No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG33140.21147679594148333No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32440.20700987508574892No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTTAGTTTGGTCCCTCCGCCGAACAC31400.20037330695722924No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT29850.1904822679195316No Hit
GTCTCTACTAGTTACTCTCCAAGCTGGTACCAGCAGACCCCAGGCCAGGC29780.19003557583395816No Hit
CTACTAGTTACTCTCCAAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACA29410.18767448909592713No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29170.18614297337396105No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT29150.1860153470637972No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28770.18359044717068423No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28260.18033597626150633No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG27390.17478423176937927No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27170.17338034235757702No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26170.166999026849385No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26010.16597801636807427No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACT25460.16246829283856867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24980.1594052613946365No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24810.15832043775824386No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24560.15672510888119587No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC24460.15608697733037666No Hit
TTATTACTGTGCGCTGTTTATCAGAGGTGGCATTTCGGTGTTCGGCGGAG24290.155002153693984No Hit
GGCCTGGGGTCTGCTGGTACCAGCTTGGAGAGTAACTAGTAGAGACTGAG24020.15327919850677219No Hit
CCCCTGGAGGGACAGTCACTCTCACTTGTGGCTTGAGCTCTGGCTCAGTC23980.1530239458864445No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23610.15066285914841346No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC23140.1476636408595632No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22740.1451111146562864No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC22510.14364341208940223No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22270.14211189636743615No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22220.14179283059202655No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGAGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC22180.14153757797169886No Hit
CAGTAATAATGAGATTCATCTTCTGCCTGGGCCCCCGTGATGGTGAGGGC21690.13841073337268478No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21400.1365601518753091No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21070.13445431775760572No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20780.13260373626023003No Hit
GACCTACGACGGTTAGTTTGGTCCCTCCGCCGAACACCGAAATGCCACCT20590.13139128631367356No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA20320.12966833112646173No Hit
CACCATCACGGGGGCCCAGGCAGAAGATGAATCTCATTATTACTGTGCGC20070.1280730022494137No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19920.12711580492318492No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19430.12398896032417082No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19300.12315938930810585No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT19040.12150024727597594No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG18830.12016017101925563No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTTAGTTTGGTCCCTCCGCCGAACACCGAAATGCCAC18770.1197772920887641No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18430.11760764481597882No Hit
CTCCAAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATC17890.11416173444155514No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC17730.11314072396024441No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGAGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG17540.11192827401368792No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTCTCCAAGCTGGTACCAGCAGACCCCAGGCCAGG17250.11007769251631225No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG16990.10841855048418232No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC16950.10816329786385465No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT16770.10701466107238006No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16730.10675940845205238No Hit
AACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGAGTGACTGTC16690.1065041558317247No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16600.10592983743598743No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG16280.103887816473366No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16210.10344112438779254No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA15740.1004419060989423No Hit
GCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATC15680.10005902716845078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.045.2227131
GTGGTAT3850.045.20471
TGGTATC4100.043.257142
GTATCAA34150.040.470491
TATCAAC37850.036.2241742
CCTAACG400.002746817434.1065942
ATCAACG40250.034.0533523
TCAACGC40800.033.502184
CGCGGTA8700.033.09088570
CAACGCA41600.032.7749185
AACGCAG41850.032.660586
CCGGTTA553.4906622E-430.99609619
ACGCAGA44550.030.8341717
TTAATCG450.004687182630.58059937
CGCAGAG45050.030.4162888
CTATAAC450.00487697230.3334391
AGAGTAC46100.029.94341511
CAGAGTA46750.029.30930510
AATGGTA7650.027.28376270
CTAACGC500.00819370627.2765673
Produced by FastQC (version 0.11.5)