FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6865
Sequences flagged as poor quality0
Sequence length22-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT145721.223597960670066No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC2042.9715950473415877No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG1331.9373634377276037No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1131.6460305899490169RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT430.6263656227239621No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT400.5826656955571741No Hit
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC390.5680990531682447No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT350.5098324836125273RNA PCR Primer, Index 27 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATGTCGTATGCCGT290.4224326292789512No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCGT290.4224326292789512No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGG200.29133284777858703No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC170.24763292061179895No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCG170.24763292061179895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160.23306627822286963No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT130.18936635105608157RNA PCR Primer, Index 27 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCC100.14566642388929352No Hit
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCT100.14566642388929352No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGACTCC90.13109978150036417No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTTTGCCGT90.13109978150036417No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCCTCCTATCTCGTATGCCGT90.13109978150036417No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCC90.13109978150036417No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCG80.11653313911143481No Hit
CTGGTAGCGCTAGCAAATCCTCCAAT80.11653313911143481No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG80.11653313911143481RNA PCR Primer, Index 27 (96% over 30bp)
AGAGACAGTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC70.10196649672250546No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGACTCCTA70.10196649672250546No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATGTCGTATGCCG70.10196649672250546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)