FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969371
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47530.4903179484428562No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38290.39499840618297843No Hit
GATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGCCCGTGGATCTGGGACAGACTTCA34950.36054307380765466No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30290.31247066396663403No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29840.30782847846696465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29240.3016388978007388No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC27050.27904692836901457No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26890.27739637352468766No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25390.26192242185912307No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24380.2515032944043096No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23830.24582951212693593No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGTCATTATTCATTATTTAGCCTGGTTTCAGCAGAAA23080.23809253629415364No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC23060.23788621693861278No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCACCAGTATGCTAATTAT22800.23520406531658158No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22600.23314087176117296No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21970.22664181206163586No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG21780.22468177818399768No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGCCCGTGGATCTGGGACAGA20600.21250893620708686No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20170.20807307006295836No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19780.20404984262991158No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19770.20394668295214113No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19120.19724130389706315No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18950.19548758937496585No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACGGGCGCTGAACCTTGATGGGAC18720.19311491678624593No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18540.19125804258637819No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18470.19053592484198517No Hit
GAGTGAAGTCTGTCCCAGATCCACGGGCGCTGAACCTTGATGGGACCCCA18380.1896074877420513No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18320.18898852967542873No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG17980.18548110063123407No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT17800.18362422643136633No Hit
GTATGCTAATTATCCGCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16800.17330825865432328No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGAGCCAGATGTGACAT16400.16918187154350606No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT16340.16856291347688346No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16020.1652618037882297No Hit
GCCTGAAGACTTTGCAACTTATTACTGCCACCAGTATGCTAATTATCCGC15340.1582469456998404No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT15300.1578343069887587No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15090.15566795375557965No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15080.1555647940778092No Hit
ATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGCCCGTGGATCTGGGACAGACTTCACTCTCA15010.1548426763334162No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCT14940.15412055858902318No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14680.15143840696699198No Hit
ACTTATTACTGCCACCAGTATGCTAATTATCCGCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA14510.14968469244489468No Hit
GTCCCAGATCCACGGGCGCTGAACCTTGATGGGACCCCAGTTTGCAAACT14410.14865309566719037No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14220.14669306178955221No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13890.143288792423128No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGAGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA13760.1419477166121124No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13650.14081296015663766No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13580.14009084241224462No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13480.13905924563454033No Hit
GTTTCTCAGGAGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA13130.13544865691257527No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC13090.13503601820149355No Hit
GATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAC12880.1328696649683145No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12820.1322507069016919No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12660.13060015205736503No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12650.1304969923795946No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12640.13039383270182417No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12540.12936223592411988No Hit
CACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCACCAGTATGCTAATTATCCGCCCACTTT12310.12698956333539999No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11960.1233789746134349No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11950.12327581493566447No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11440.11801467136937252No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11380.11739571330274992No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11240.11595147781396391No Hit
ACCTTGATGGGACCCCAGTTTGCAAACTGGATGCAGCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTG11230.11584831813619346No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11050.11399144393632572No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10920.11265036812531012No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGC10910.1125472084475397No Hit
GCCCCTAAGTCCCTGATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGCCCGTGGAT10850.11192825038091711No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10810.11151561166983538No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10690.11027769553659023No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10630.10965873746996764No Hit
CATCAAGGTTCAGCGCCCGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTG10580.10914293908111548No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10580.10914293908111548No Hit
ACCTTGGTCCCTCCGCCGAAAGTGGGCGGATAATTAGCATACTGGTGGCAGTAATAAGTTGCAAAGTCTTCAGG10510.10842082133672246No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10440.10769870359232947No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10320.10646078745908429No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10230.10553235035915041No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10180.10501655197029827No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG10150.10470707293698699No Hit
ATGCAGCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAATGAATAATGAC10050.10367547615928267No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACGGGCG10040.10357231648151224No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10030.1034691568037418No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9960.10274703905934879No Hit
GATAATTAGCATACTGGTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGT9950.10264387938157837No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9890.10202492131495579No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9860.1017154422816445No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAATGAATAA9820.10130280357056277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1050.046.05576740
CGTATGC1000.044.94913541
GGTATCA7800.042.277821
CACGAGA1100.040.17986721
GTGCTAG1400.038.853251
ACCGAAC350.001449051838.851258
TATCTCG1100.037.42698336
GCCTATC1100.037.31238633
ATCTCGT1200.037.2232737
ACGAGAC1200.036.83534622
GTATCAA20100.035.3496931
CGTACCC400.002790141633.9965933
ATCTCCG1350.032.73577510
CCCACGA1350.032.73577519
GTCGGTT550.003990823431.600469
TATCAAC22100.031.3814742
GTATGCC1450.031.0596942
TCTCCGA1450.030.47813411
ATCAACG22950.029.922933
TCAACGC23000.029.8578784
Produced by FastQC (version 0.11.5)