FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969371
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47710.492174822642724No Hit
GATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGCCCGTGGATCTGGGACAGACTTCA34420.35507561088582185No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31930.3293888511209846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31570.3256751027212491No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31370.32361190916584054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27710.285855467101863No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27060.279150088046785No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC24040.24799586536011498No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23700.24448843631592032No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGTCATTATTCATTATTTAGCCTGGTTTCAGCAGAAA23030.23757673790530145No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23020.23747357822753104No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22830.23551354434989286No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22770.23489458628327026No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCACCAGTATGCTAATTAT22210.22911764432812617No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22050.22746708948379926No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21850.2254038959283907No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGCCCGTGGATCTGGGACAGA20990.21653216364013367No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC20260.20900150716289223No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACGGGCGCTGAACCTTGATGGGAC19800.2042561619854524No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19520.20136769100788038No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19160.19765394260814487No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19140.19744762325260404No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18640.1922896393640825No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18370.18950432806428086No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG18240.18816325225326527No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18060.18630637805339753No Hit
GAGTGAAGTCTGTCCCAGATCCACGGGCGCTGAACCTTGATGGGACCCCA17990.18558426030900452No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17790.1835210667535959No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16510.17031662799898079No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16440.16959451025458777No Hit
GTATGCTAATTATCCGCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16440.16959451025458777No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT16130.16639656024370442No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16080.16588076185485226No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15720.16216701345511678No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT15710.16206385377734633No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT15400.158865903766463No Hit
GCCTGAAGACTTTGCAACTTATTACTGCCACCAGTATGCTAATTATCCGC15250.15731850859990654No Hit
ATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGCCCGTGGATCTGGGACAGACTTCACTCTCA14920.15391423923348233No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14770.15236684406692586No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGAGCCAGATGTGACAT14630.15092260857813986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14450.14906573437827209No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14410.14865309566719037No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14400.14854993598941996No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGAGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA14360.14813729727833821No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14350.14803413760056777No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCT14280.1473120198561748No Hit
ACTTATTACTGCCACCAGTATGCTAATTATCCGCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13700.14132875854548982No Hit
GTCCCAGATCCACGGGCGCTGAACCTTGATGGGACCCCAGTTTGCAAACT13550.13978136337893335No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC13360.13782132950129516No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13310.13730553111244304No Hit
GTTTCTCAGGAGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA12820.1322507069016919No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12820.1322507069016919No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12580.12977487463520157No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12500.12894959721303814No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12190.1257516472021548No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12120.1250295294577618No Hit
GATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAC12050.12430741171336877No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11770.1214189407357967No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11660.12028418428032199No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG11590.11956206653592896No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11540.11904626814707682No Hit
CACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCACCAGTATGCTAATTATCCGCCCACTTT11360.11718939394720906No Hit
ACCTTGATGGGACCCCAGTTTGCAAACTGGATGCAGCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTG11230.11584831813619346No Hit
GCCCCTAAGTCCCTGATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGCCCGTGGAT10970.11316616651416227No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT10850.11192825038091711No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10750.11089665360321281No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10750.11089665360321281No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACGGGCG10710.11048401489213108No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10680.11017453585881978No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGC10630.10965873746996764No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10630.10965873746996764No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10620.10955557779219721No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10340.10666710681462516No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10280.10604814874800259No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10210.10532603100360956No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9720.10027120679285846No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9700.10006488743731762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGT200.00753394151.44278736
GTGGTAT2850.040.4384841
GGTATCA8000.038.981511
CTAGGGA459.093318E-538.96639649
TGGTATC3200.036.0062332
GTATCAA20800.035.6895941
TATCAAC21950.033.1934742
ATCAACG23150.031.4679933
ATGGCAA3850.031.09725670
TCAACGC23550.031.0757774
CGAACTA553.518389E-430.95279929
CAACGCA23800.030.7556975
AACGCAG23750.030.679346
ATAGTAT450.005048851530.11823
GTGCTAG2150.029.9554581
GTACGTA600.005770448629.3053869
ACGTACC2100.029.0590413
TACGTAC2100.029.0590412
GCGCAAT500.006347156628.74020661
CGCAGAG25600.028.7344578
Produced by FastQC (version 0.11.5)