FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570908
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAACTCAGGTTCAGGGAGA54200.34502338774772295No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37770.24043419474596858No Hit
GAGTATTACTGCCTGCTCTACTTTGGTGGTGCTCAGCCCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC36950.23521428371362293No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA34840.22178256142307506No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33970.2162443631326596No Hit
GATTTATAGTACAAGCAACAAACCCTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC33290.21191565642290955No Hit
GTATTACTGCCTGCTCTACTTTGGTGGTGCTCAGCCCAGGTGGGTGTTCG33240.2115973691648397No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31450.2002026853259389No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG30100.1916089293580528No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGGGCTGA27760.17671308568038357No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27720.1764584558739277No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT27620.17582188135778798No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA27380.17429410251905267No Hit
GTCTCTGGTGGCTCCATCAGTAGTGGCAATTACTACTGGAGCTGGATCCG26020.16563668909955262No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25610.1630267335833798No Hit
GTATATTACTGTGCGAGAACCTCCTGGAATGATAAGTCCGAAGGGGGTCC24820.1579977949058761No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24740.15748853529296433No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC24610.1566609884219827No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24610.1566609884219827No Hit
GCCCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT24530.15615172880907094No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC24140.15366908819612607No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCGGGAGGGACAGTCAC23780.15137741993802312No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23510.1496586687444459No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23320.14844917716378042No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23260.14806723245409661No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22930.14596653655083558No Hit
ACTTTGGTGGTGCTCAGCCCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG22210.14138320003462967No Hit
CCACTGGAACAGTCACCAGTGCTTCCTTTCCAAACTGGTTCCAGCAGAAA22100.14068296806687597No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21970.13985542119589434No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG21740.13839129980877302No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21510.1369271784216517No Hit
CTATAAATCAGTGGCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGAAAGGAAGCACTGGTGACTGTT21070.13412625055063696No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20910.13310773132481343No Hit
GTCCAGGAGGGTTTGTTGCTTGTACTATAAATCAGTGGCCTGGGTGCTTG20720.13189823974414797No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20680.13164360993769209No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20550.13081606306671045No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC19960.12706027342148618No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19900.12667832871180235No Hit
CACCTGGGCTGAGCACCACCAAAGTAGAGCAGGCAGTAATACTCAGCCTC19660.12515054987306704No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19470.1239410582924016No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19310.12292253906657806No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19280.12273156671173614No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19210.12228596455043834No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18780.1195486941310376No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCGGGAGGGACAGTCACTCTCAC18490.11770262803423243No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18300.11649313645356697No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18200.11585656193742727No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17990.11451975545353388No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17990.11451975545353388No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17990.11451975545353388No Hit
CCACCAAAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTG17930.11413781074385006No Hit
CACTGACTGTGTCCCCGGGAGGGACAGTCACTCTCACCTGTGCTCCCACC17520.11152785522767725No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTTTGGTGGTGCTC17300.11012739129216988No Hit
GGACTGGAGTGGGTTGGGCGTATTTATCCCAGTGGGAGCACCACCTACAA16460.10478016535659632No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16410.10446187809852645No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTGGCAATTACTACTGGA16210.10318872906624704No Hit
CTCACTGACTGTGTCCCCGGGAGGGACAGTCACTCTCACCTGTGCTCCCACCACTGGAACAGTCACCAGTGCTTC16130.10267946945333527No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15920.10134266296944187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)