FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570908
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAACTCAGGTTCAGGGAGA49670.3161865621665941No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37730.2401795649395127No Hit
GAGTATTACTGCCTGCTCTACTTTGGTGGTGCTCAGCCCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC36260.23082191955225898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35850.22821196403608615No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34010.21649899293911548No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33430.21280686074550514No Hit
GATTTATAGTACAAGCAACAAACCCTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC31700.20179412161628815No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA30730.19561934880973297No Hit
GTATTACTGCCTGCTCTACTTTGGTGGTGCTCAGCCCAGGTGGGTGTTCG29840.18995383561608956No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA29190.18581610126118142No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG27480.17493067703519238No Hit
GTCTCTGGTGGCTCCATCAGTAGTGGCAATTACTACTGGAGCTGGATCCG26540.16894687658347912No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26120.16627326361569233No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCGGGAGGGACAGTCAC25020.15927094393815552No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGGGCTGA24830.15806145235749006No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT24630.15678830332521065No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24470.1557697840993871No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC24040.15303251367998635No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23950.1524595966154606No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23510.1496586687444459No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC23330.1485128346153944No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23220.1478126026476407No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22910.14583922164760763No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG22390.14252903416368112No Hit
GCCCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT22360.14233806180883923No Hit
GTATATTACTGTGCGAGAACCTCCTGGAATGATAAGTCCGAAGGGGGTCC22290.1418924596475414No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22060.1404283382604201No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22060.1404283382604201No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21150.13463551016354872No Hit
CCACTGGAACAGTCACCAGTGCTTCCTTTCCAAACTGGTTCCAGCAGAAA19970.12712393087310014No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC19830.12623272655050455No Hit
ACTTTGGTGGTGCTCAGCCCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG19640.12502323496983908No Hit
GTCCAGGAGGGTTTGTTGCTTGTACTATAAATCAGTGGCCTGGGTGCTTG19410.12355911358271778No Hit
CTATAAATCAGTGGCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGAAAGGAAGCACTGGTGACTGTT19360.1232408263246479No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19330.12304985396980599No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19260.12260425180850822No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19030.12114013042138687No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18930.12050355590524715No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18820.11980332393749349No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18810.11973966648587951No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18390.11706605351809271No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCGGGAGGGACAGTCACTCTCAC18380.11700239606647876No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18020.1147107278083758No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17830.11350123622771034No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17720.11280100425995668No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17060.10859961245343458No Hit
CACCTGGGCTGAGCACCACCAAAGTAGAGCAGGCAGTAATACTCAGCCTC16970.10802669538890883No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16790.10688086125985735No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16480.10490748025982426No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16350.10407993338884262No Hit
CACTGACTGTGTCCCCGGGAGGGACAGTCACTCTCACCTGTGCTCCCACC16240.10337970142108896No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15980.10172460767912571No Hit
CCACCAAAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTG15760.10032414374361834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)