FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258412
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCACTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA82300.6539988493434582No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72800.5785068800996812No Hit
GTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC62720.4984059274704945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60800.4831486031601733No Hit
CTATTAAGCTGCTGACAGTAATAAGTTGCAAAATCCTGAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC57360.45581256377084767No Hit
GCTTAATAGTTACCCTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT55520.4411909613067898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48390.3845322517585656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48250.38341973852760464No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44510.35369974221479134No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42650.33891920928916763No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40610.3227083022094513No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39810.3163510837468174No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38150.30315985543685214No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35750.2840882000489506No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT35670.28345247820268715No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35650.28329354774112137No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTGGCAGTTCTTTAGGCTGGTATCAGCAGAAA35480.2819426388178117No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT34940.2776515163555338No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34630.27518809420126317No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34240.2720889502007292No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG33760.26827461912314887No Hit
GTGCAAAGTGGATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAGAA32700.259851304660159No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32660.2595334437370273No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG32480.25810306958293466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32310.256752160659625No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30570.24292521050339635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29720.23617066588684787No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29690.23593227019449908No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29090.2311643563475237No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29040.2307670301936091No Hit
AGTCAGGACATTGGCAGTTCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGATCTATA28980.23029023880891153No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT28940.22997237788577987No Hit
GGACCCCACTGTGCAAAGTGGATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGC28160.22377408988471184No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25660.20390778218898103No Hit
ATACTGCATCCACTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA24400.19389516311033272No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23360.18563077910890868No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22920.18213430895446006No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTCAGGATTTTGCAACTTATTACTGTCAGCAGCTTAATAGTTAC22910.18205484372367714No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22670.180147678184887No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22360.17768425603061638No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22220.17657174279965543No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21810.1733136683375556No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC21710.17251901602972636No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21690.17236008556816051No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21650.17204222464502883No Hit
CACCATCAGCAGCCTGCAGCCTCAGGATTTTGCAACTTATTACTGTCAGCAGCTTAATAGTTACCCTCTCACCTT20490.16282425787420973No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19620.1559107827960954No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19530.1551955957190491No Hit
TCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGAT19470.15471880433435156No Hit
ACTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGC19330.15360629110339064No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19250.15297056925712724No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG19200.1525732431032126No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19120.15193752125694923No Hit
GTCGTGTCCCTTGGCCGAAGGTGAGAGGGTAACTATTAAGCTGCTGACAGTAATAAGTTGCAAAATCCTGAGG19050.15138126464146875No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19040.15130179941068583No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18580.14764639879467137No Hit
CAGTAATAAGTTGCAAAATCCTGAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG18210.14470618525570322No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18190.14454725479413735No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18030.14327581110161058No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18020.14319634587082766No Hit
CACTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAG17890.14216329787064969No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17830.14168650648595213No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCA17470.1388257581777669No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17450.13866682771620106No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17430.13850789725463522No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17430.13850789725463522No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17390.1381900363315035No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17330.13771324494680598No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17000.1350908923309695No Hit
GCCTAAAGAACTGCCAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTC16810.13358105294609396No Hit
GGACATTGGCAGTTCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGATCTATACTGCATC16710.13278640063826472No Hit
ACTTATTACTGTCAGCAGCTTAATAGTTACCCTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA16430.1305613741763429No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT16380.13016404802242826No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCAA16250.12913100002225028No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16180.1285747434067698No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16170.12849527817598688No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16070.12770062586815764No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC16050.1275416954065918No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16010.1272238344834601No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15960.1268265083295455No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15850.12595239079093334No Hit
ATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAGAACTGCCAATGTC15710.12483987755997242No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15460.12285324679039934No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTCAGGATTTTG15330.12182019879022132No Hit
CCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG15060.11967463755908239No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA14940.11872105478968732No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14930.1186415895589044No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14870.11816479817420686No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14860.11808533294342394No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14690.11673442402011425No Hit
GTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCAACTGGATGGCACATCTGGCACCTGGGAGCCAGAG14670.11657549355854838No Hit
GCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATT14590.11593977171228499No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14590.11593977171228499No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14520.11538351509680456No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14390.11435046709662655No Hit
GTAATAAGTTGCAAAATCCTGAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG14320.11379421048114607No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14150.11244330155783638No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14050.11164864925000716No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14020.11141025355765838No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTCAGGATT13920.11061560124982915No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13660.10854950524947315No Hit
ACCTTGATGGGACCCCACTGTGCAAAGTGGATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTG13660.10854950524947315No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA13490.10719859632616344No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13480.10711913109538052No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGATCTATACTGCATCCACTTTGCACAGTGGGGTCCCAT13280.10552982647972207No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGAGAGGGTAA13200.10489410463345869No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13150.10449677847954406No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12950.10290747386388559No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC12850.10211282155605636No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12840.10203335632527344No Hit
TTATTACTGTCAGCAGCTTAATAGTTACCCTCTCACCTTCGGCCAAGGGA12810.10179496063292467No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCAAATATA12790.10163603017135882No Hit
GTGGATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTG12590.10004672555570036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA4000.064.8279470
CCTTAGA200.007351089351.76434741
TGATCGT200.008045088550.5965127
TAGCGCT3000.045.0590929
CTTATAC3150.044.8406261
ATCTCGT2800.043.91811437
TCGTATG2750.043.8229740
CTAGCGC3100.043.5620828
CGTATGC2700.043.45648641
GTGGTAT2600.042.6848261
TACGTAT659.881941E-441.99380570
CGCTCAT3100.041.58971832
ACTAGCG3250.041.51508327
AGCGCTC3100.041.47166430
TATACAC3500.041.3174363
GACTAGC3300.040.84536426
TTATACA3450.039.9666442
ATGCCGT3050.039.87595744
CACGAGA3300.039.75615721
ATCTCCG3300.039.737210
Produced by FastQC (version 0.11.5)