FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258412
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCACTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA77490.6157760733368721No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73100.5808908370231689No Hit
GTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC59000.4688448616192471No Hit
CTATTAAGCTGCTGACAGTAATAAGTTGCAAAATCCTGAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC57000.45295181546266244No Hit
GCTTAATAGTTACCCTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT51940.41274240868650325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51790.4115504302247595No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51710.41091470837849603No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT47550.3778571723728No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47400.3766651939110562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43370.34464070590553814No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40050.3182582492856076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39990.31778145790091006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39610.314761779131159No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35850.28488285235677985No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35650.28329354774112137No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34950.2777309815863167No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTGGCAGTTCTTTAGGCTGGTATCAGCAGAAA34260.27224788066229505No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT33860.2690692714309781No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33650.26740050158453665No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG33640.26732103635375376No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32880.26128167881425163No Hit
GTGCAAAGTGGATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAGAA32190.2557985778902299No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32100.2550833908131836No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30700.24395825850357433No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29670.23577333973293324No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29660.23569387450215032No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28570.2270321643468117No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28510.22655537296211417No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27730.22035708496104617No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27390.21765526711442673No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26620.21153644434414165No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26370.2095498135745686No Hit
AGTCAGGACATTGGCAGTTCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGATCTATA25560.2031131298811518No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT25350.2014443600347104No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25080.1992987988035715No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24500.19468981541816194No Hit
GGACCCCACTGTGCAAAGTGGATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGC23780.18896831880179146No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23480.18658436187830377No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23080.18340575264698683No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC22690.18030660864645284No Hit
ATACTGCATCCACTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA22390.17792265172296512No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTCAGGATTTTGCAACTTATTACTGTCAGCAGCTTAATAGTTAC21830.17347259879912144No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21330.1694993372599753No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21080.16751270649040217No Hit
GTCGTGTCCCTTGGCCGAAGGTGAGAGGGTAACTATTAAGCTGCTGACAGTAATAAGTTGCAAAATCCTGAGG20840.16560554095161203No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20690.1644135624898682No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20170.1602813704891562No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC20040.15924832248897816No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19700.15654650464235878No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19650.15614917848844417No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19480.15479826956513448No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19450.15455987387278572No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18930.1504276818720737No Hit
CACCATCAGCAGCCTGCAGCCTCAGGATTTTGCAACTTATTACTGTCAGCAGCTTAATAGTTACCCTCTCACCTT18920.15034821664129078No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18760.149076772948764No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG18650.14820265541015185No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCA18290.14534190710196662No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA18180.14446778956335443No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17840.14176597171673505No Hit
ACTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGC17700.14065345848577412No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17630.14009720187029367No Hit
CAGTAATAAGTTGCAAAATCCTGAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG17440.13858736248541814No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCAA17120.1360444751003646No Hit
TCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGAT16920.13445517048470612No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16910.1343757052539232No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16770.13326319202296227No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16770.13326319202296227No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16620.13207121356121843No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16450.13072030463790874No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16190.12865420863755272No Hit
ACTTATTACTGTCAGCAGCTTAATAGTTACCCTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA16070.12770062586815764No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15780.12539613417545287No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15710.12483987755997242No Hit
GTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCAACTGGATGGCACATCTGGCACCTGGGAGCCAGAG15580.12380682955979441No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15560.12364789909822857No Hit
CACTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAG15470.12293271202118226No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15440.12269431632883347No Hit
GGACATTGGCAGTTCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGATCTATACTGCATC15440.12269431632883347No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15340.12189966402100424No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15330.12182019879022132No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15290.12150233786708962No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15160.12046928986691163No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15120.12015142894377993No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC14940.11872105478968732No Hit
GTAATAAGTTGCAAAATCCTGAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG14940.11872105478968732No Hit
GCCTAAAGAACTGCCAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTC14860.11808533294342394No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14770.11737014586637763No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14570.11578084125071916No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14410.11450939755819238No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14200.11284062771175099No Hit
ATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAGAACTGCCAATGTC14190.11276116248096807No Hit
CCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG14140.11236383632705346No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14120.1122049058654876No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13620.10823164432634146No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13600.10807271386477561No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTCAGGATTTTG13580.10791378340320977No Hit
GTGGATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTG13380.1063244787875513No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13230.10513250032580744No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGAGAGGGTAA13150.10449677847954406No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTG13100.10409945232562944No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13060.10378159140249775No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13050.10370212617171483No Hit
GCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATT12940.10282800863310267No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12830.10195389109449052No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTCAGGATT12640.10044405170961497No Hit
ACCTTGATGGGACCCCACTGTGCAAAGTGGATGCAGTATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTG12630.10036458647883205No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTC12620.10028512124804913No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTGTGCAAAGT12610.1002056560172662No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12610.1002056560172662No Hit
GGTGAGAGGGTAACTATTAAGCTGCTGACAGTAATAAGTTGCAAAATCCT12590.10004672555570036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)