FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848640
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC48000.5656108597285068No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36860.4343420060331825No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT31130.366822209653092No Hit
CCCATATACAACACACAATAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC30000.35350678733031676No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29310.34537613122171945No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29200.34407993966817496No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAACACA28440.33512443438914025No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG26240.3092006033182504No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT26040.30684389140271495No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG25830.30436934389140274No Hit
CTCATCTACAGCACAAACAGTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG25270.29777055052790347No Hit
CCCCAGAAGAGCGACTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG22360.26348039215686275No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22340.2632447209653092No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAACACACAATAATAAT22170.26124151583710403No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21710.25582107843137253No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20290.2390884238310709No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20260.23873491704374059No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19850.2339036576168929No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19560.23048642533936653No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19360.2281297134238311No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18680.22011689291101055No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18600.21917420814479638No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18030.21245757918552036No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG17900.21092571644042232No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT17400.20503393665158373No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG17270.2035020739064857No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17060.20102752639517343No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG16840.19843514328808445No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC16460.19395739064856712No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACAGTCGCTCT16350.19266119909502263No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16080.18947963800904977No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16030.1888904600301659No Hit
GTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC16030.1888904600301659No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA16020.18877262443438914No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15750.18559106334841627No Hit
GATTATTATTGTGTGTTGTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT15530.1829986802413273No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15470.18229166666666666No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15400.18146681749622925No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15380.1812311463046757No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG15300.18028846153846154No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15280.180052790346908No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14940.17604638009049775No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC14810.17451451734539972No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14750.17380750377073906No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14590.1719221342383107No Hit
CTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCA14580.17180429864253394No Hit
ATAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG14430.17003676470588236No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13900.16379147812971342No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13650.1608455882352941No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13070.15401112368024134No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC13020.15342194570135748No Hit
AACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTC12960.15271493212669685No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12640.14894419306184012No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC12630.14882635746606335No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12530.14764800150829563No Hit
CCCAAATGCCACTACCCATATACAACACACAATAATAATCAGATTCATCA12420.14635180995475114No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT12400.1461161387631976No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT12330.1452912895927602No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12280.14470211161387633No Hit
AGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTCTGAGAAT12280.14470211161387633No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA12240.14423076923076925No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC12230.14411293363499247No Hit
CATCTACAGCACAAACAGTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC12150.14317024886877827No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC12140.1430524132730015No Hit
ACCCATATACAACACACAATAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT11970.14104920814479638No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC11730.13822115384615385No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG11660.13739630467571645No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA11220.13221153846153846No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG11210.1320937028657617No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11190.13185803167420815No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11130.1311510180995475No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11040.13009049773755654No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10980.1293834841628959No Hit
GAGGAAAACAAACCCCAGCTGGGAAGCCTGAGAACACTTAGCCTTCATGA10830.12761595022624433No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10800.12726244343891402No Hit
CTGTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG10600.12490573152337857No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC10590.1247878959276018No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG10340.1218420060331825No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10190.12007447209653092No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10120.11924962292609352No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCA10070.11866044494720965No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10040.11830693815987933No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9950.11724641779788839No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9880.11642156862745098No Hit
GTGTTGTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG9840.1159502262443439No Hit
GGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGAGCGACTGTTTGTGCTGTAGATGAGCGTGCGTGGAGC9780.11524321266968326No Hit
GCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGA9770.11512537707390649No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTAC9640.11359351432880846No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTA9540.11241515837104071No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG9370.11041195324283559No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG9230.10876225490196079No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9210.10852658371040724No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9190.1082909125188537No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9160.10793740573152338No Hit
GCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAACACACAATAATAATCAGATTCATCATCTG9070.10687688536953244No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTATTGTGTGT8870.10452017345399699No Hit
GGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGAGCGACTGTTTGTG8860.10440233785822021No Hit
CTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAA8850.10428450226244344No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8730.10287047511312218No Hit
CTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGA8650.101927790346908No Hit
GGTCACCACAGTCTGAGAATCCACTCCTGATCCATAAGCAAGGAGTCCGA8530.10051376319758672No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8500.1001602564102564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)