FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691803
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC73000.4314923191411766No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCG72230.4269409618022902No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAATGCCAGTGGCCCAGGTCTGACAGTAATAGTCAG68710.4061347568245239No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG60480.35748843098162136No Hit
CTATTAAGCCTCATCAAGAATCGAGGGCCCTTCTCTGGCCGCTGTTGATGCCATGCGATGGTGTAGCTTCTGTGC59140.3495678870412217No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT52990.3132161368669993No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50500.2984981111867044No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC48870.2888634196771137No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC45560.2692984939735891No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC43970.2599002366114731No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT40050.2367296901589606No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG37700.22283918399482683No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT37420.22118414496250452No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG36860.21787406689785985No Hit
GACTATTACTGTCAGACCTGGGCCACTGGCATTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG36730.21710565591856737No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT36700.2169283303079614No Hit
GGCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGC35650.21072193393675268No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT35020.20699809611402745No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34920.20640701074534093No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA34580.20439732049180667No Hit
CCTGCACTCTGAGCAGTGGGCACAGAAGCTACACCATCGCATGGCATCAA34230.20232852170140375No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34110.2016192192589799No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAATGCCAGTGGCCCAGGTCTGACAGTAATAGTCAGC34030.20114635096403066No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGC33980.2008508082796874No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33350.19712697045696218No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32910.1945261948347414No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC32840.19411243507666082No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31640.1870194106524223No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA31140.18406398380898958No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30100.17791669597464954No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG30080.17779847890091222No Hit
GCTCTGGGGCTGAGCGCCACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC29740.17578878864737796No Hit
GGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC29480.17425196668879295No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT29160.17236049350899602No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28930.17100099716101697No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC28610.16910952398122003No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28510.1685184386125335No Hit
CACCTGCACTCTGAGCAGTGGGCACAGAAGCTACACCATCGCATGGCATC26570.15705138246001454No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26540.15687405684940858No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGCCACTGGCATTCGAGTATTCGGCGGAGGGACCAAG26410.15610564587011608No Hit
CTATTACTGTCAGACCTGGGCCACTGGCATTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC26170.1546870409852684No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG25850.15279556780547146No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC24690.14593897752870755No Hit
CTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT24600.14540700069688964No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC24210.14310176775901212No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC24200.14304265922214346No Hit
GAAGCTACACCATCGCATGGCATCAACAGCGGCCAGAGAAGGGCCCTCGA23950.1415649458004271No Hit
GCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT23760.14044188359992268No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23660.13985079823123614No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGCGCTCAGCCCCAGAGCTGGAGC23400.13831397627265113No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCCACCTCACCATCTCCAG23360.1380775421251765No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23270.13754556529335862No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23150.13683626285093478No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT23130.13671804577719746No Hit
GCATCAACAGCGGCCAGAGAAGGGCCCTCGATTCTTGATGAGGCTTAATAGTGATGGCAGCCACAGCAAGGGGGA23090.13648161162972283No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22480.13287599088073493No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22010.1300978896479082No Hit
GTAATACACAGCCGTGTCGTCGTCTCTCAGGCTGCTCATTTGCAGATACA21800.12885661037366644No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21190.12525098962467854No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21090.12465990425599197No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20970.12395060181356814No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20810.12300486522366966No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20150.11910370179033848No Hit
GTGTAGCTTCTGTGCCCACTGCTCAGAGTGCAGGTGAGCTTGACCGAGGC20100.1188081591059952No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19840.11727133714741018No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19770.1168575773893296No Hit
GGCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG19670.11626649202064307No Hit
CTCCAGCTCTGGGGCTGAGCGCCACCTCACCATCTCCAGCCTCCAGTCTG19660.1162073834837744No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCT19290.1140203676196342No Hit
GTGTATTACTGTGCGAGAGATCCCCTTGACTACAATTATGTCGACTACTGGGGCCAGGGAATCCTGGTCACCGT19260.11384304200902824No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT19140.11313373956660439No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18570.10976455296509109No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT18550.10964633589135378No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18500.1093507932070105No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18350.1084641651539807No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18300.10816862246963742No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18240.10781397124842551No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18140.10722288587973895No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18140.10722288587973895No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18120.10710466880600165No Hit
GCTTGGTCCCTCCGCCGAATACTCGAATGCCAGTGGCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGA17990.10633625782670914No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17820.10533141269994202No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC17730.10479943586812412No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC17480.10332172244640776No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCTGTGGCT17430.10302617976206449No Hit
GAGAAGGGCCCTCGATTCTTGATGAGGCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCG17340.10249420293024661No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGA17230.10184400902469141No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGC17030.10066183828731831No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA200.00794419850.759089
TATACGC200.00794419850.759087
GGTATCA14600.045.895841
CGCTAAT1050.042.14424532
TCTCGTA1100.040.60279538
ATCTCGT1200.040.01141437
GTATCAA38250.038.1335141
TCGCTAA1200.036.82913631
TCGTATG1250.035.8628240
CGTATGC1300.034.54694741
GTGGTAT4050.034.2601971
TGGTATC4150.034.2501262
ACTCGCT1350.032.68557429
ACGAGAC1450.032.67733822
TATCAAC46000.031.7824152
ATCAACG46550.031.6250043
TCAACGC46900.031.3889944
CAACGCA47600.030.9273935
TCTGGAG9200.030.91965570
ACGGGTT553.6467542E-430.7658065
Produced by FastQC (version 0.11.5)