FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691803
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCG69570.4112180909952282No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC68350.4040068494972523No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAATGCCAGTGGCCCAGGTCTGACAGTAATAGTCAG62100.3670640139543434No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG54930.3246831930195182No Hit
CTATTAAGCCTCATCAAGAATCGAGGGCCCTTCTCTGGCCGCTGTTGATGCCATGCGATGGTGTAGCTTCTGTGC54280.3208411381230557No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53000.3132752454038679No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50490.29843900264983575No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC48940.28927717943519426No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41410.24476845117309756No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG41340.244354691415017No Hit
GGCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGC40840.2413992645715843No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC40150.2373207755276471No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39080.2309961620827011No Hit
GGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC37400.2210659278887672No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36880.21799228397159717No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT36320.21468220590695253No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT35600.2104263912524094No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG34690.20504751439736185No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT34550.2042199948812007No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA34480.20380623512312016No Hit
GACTATTACTGTCAGACCTGGGCCACTGGCATTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG34240.20238763023827244No Hit
CCTGCACTCTGAGCAGTGGGCACAGAAGCTACACCATCGCATGGCATCAA32850.19417154361352945No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32660.19304848141302502No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32380.19139344238070272No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31800.18796514724232077No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT31760.18772871309484615No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC31490.18613278259939248No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGC30880.18252716185040457No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA30040.1775620447534376No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT29520.17448840083626757No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG29420.17389731546758103No Hit
GCTCTGGGGCTGAGCGCCACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC29280.17306979595141989No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC29040.17165119106657217No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTCGAATGCCAGTGGCCCAGGTCTGACAGTAATAGTCAGC28800.17023258618172446No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGCCACTGGCATTCGAGTATTCGGCGGAGGGACCAAG28270.16709983372768578No Hit
CTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT27090.16012502637718456No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26790.15835177027112493No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26160.15462793244839973No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG26120.1543914983009251No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC25790.1524409165842595No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25090.1483033190034537No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24700.1459980860655762No Hit
CTATTACTGTCAGACCTGGGCCACTGGCATTCGAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC24690.14593897752870755No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24510.14487502386507176No Hit
CACCTGCACTCTGAGCAGTGGGCACAGAAGCTACACCATCGCATGGCATC24350.1439292872751733No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC24290.14357463605396137No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23990.14180137994790173No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23480.13878684456760038No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCCACCTCACCATCTCCAG23070.13636339455598553No Hit
GCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT22950.1356540921135617No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22870.13518122381861244No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT22790.1347083555236632No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22430.13258044819639164No Hit
GAAGCTACACCATCGCATGGCATCAACAGCGGCCAGAGAAGGGCCCTCGA22300.13181203721709916No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGCGCTCAGCCCCAGAGCTGGAGC21410.12655137743578893No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC20950.12383238473983081No Hit
GCATCAACAGCGGCCAGAGAAGGGCCCTCGATTCTTGATGAGGCTTAATAGTGATGGCAGCCACAGCAAGGGGGA20890.1234777335186189No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20680.12223645424437717No Hit
GTAATACACAGCCGTGTCGTCGTCTCTCAGGCTGCTCATTTGCAGATACA20430.12075874082266079No Hit
GTGTTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG20330.12016765545397425No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20280.11987211276963097No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20110.11886726764286386No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20080.1186899420322579No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20040.11845350788478327No Hit
CTCCAGCTCTGGGGCTGAGCGCCACCTCACCATCTCCAGCCTCCAGTCTG19900.11762598836862212No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19830.11721222861054155No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19830.11721222861054155No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19760.11679846885246095No Hit
GTGTATTACTGTGCGAGAGATCCCCTTGACTACAATTATGTCGACTACTGGGGCCAGGGAATCCTGGTCACCGT19710.1165029261681177No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCT19310.11413858469337151No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19080.11277908834539246No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC18870.11153780907115071No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18260.10793218832216281No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCTGC18070.10680912612165837No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT17750.10491765294186144No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17620.10414924196256892No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA17560.10379459074135701No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17510.10349904805701372No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC17480.10332172244640776No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17360.1026124200039839No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA17350.10255331146711527No Hit
GCTTGGTCCCTCCGCCGAATACTCGAATGCCAGTGGCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGA17270.10208044317216602No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17260.10202133463529738No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC17250.10196222609842871No Hit
GTGTAGCTTCTGTGCCCACTGCTCAGAGTGCAGGTGAGCTTGACCGAGGC17250.10196222609842871No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCTGTGGCT17180.10154846634034813No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17160.10143024926661083No Hit
GAGAAGGGCCCTCGATTCTTGATGAGGCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCG17080.1009573809716616No Hit
GGCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG17050.10078005536105564No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16940.10012986145550044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG400.006763054552.8657570
TTCGTAA200.0079580450.7367312
GTGGTAT4750.044.1159131
GGTATCA15500.042.3026161
TGGTATC5150.039.3605842
GTATCAA38800.037.718411
TATCAAC44850.032.4662632
ATCAACG45950.031.7625753
TCAACGC46150.031.5517224
CAACGCA46950.031.0869735
AACGCAG47650.030.6375376
CGCAGAG51300.028.4102768
ACGCAGA51450.028.3173967
AGAGTAC52900.027.81408511
CAGAGTA52900.027.61981210
GATCGGA500.00781678227.54122539
GCAGAGT53350.027.3836029
TTAACGG1209.286123E-825.64688135
TCTGGAG10050.024.54794370
GGTAGAA40900.024.4724970
Produced by FastQC (version 0.11.5)