FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159269
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51180.4414851082880677No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41690.35962317632922125No Hit
ACAGTACCCTGTGGACGTTCGGCCACGGGACCAAGGTGGTAATCAAACGA40190.3466839879268746No Hit
GGGTACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAAT39350.33943804242156045No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35610.3071763326717095No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCT34520.29777385576600424No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32120.27707115432224966No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30060.25930133558302687No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29990.2586975067909174No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAAA29400.2536080926859944No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29260.25240043510177534No Hit
GACTTACAGTACCCTGTGGACGTTCGGCCACGGGACCAAGGTGGTAATCAAACGAACTGTGGCTGCACCAT28780.2482598948130244No Hit
GGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG28180.24308421945208578No Hit
CCTTGGTCCCGTGGCCGAACGTCCACAGGGTACTGTAAGTCTGTTGACAG25500.21996620283989304No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25450.21953489655981484No Hit
GGACCAAGGTGGTAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC25130.21677453636731425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25130.21677453636731425No Hit
GTACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAAT24790.21384165366278232No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24560.2118576447744225No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24540.21168512226239122No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24250.20918354583793755No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCATTAGCAG23940.20650944690145256No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23690.20435291550106144No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22940.19788332129988812No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22060.19029233077051141No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21990.18968850197840192No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21810.18813579937012032No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAATCTGTC21580.1861517904817605No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21410.18468534912949452No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGCTGCTAATGCTCTGAC21350.18416778159340066No Hit
GTGCAGCCACAGTTCGTTTGATTACCACCTTGGTCCCGTGGCCGAACGTC21040.1814936826569157No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTACCACCTTGGTCCCGTGGCCGAACGTCCACAGGGTA20040.17286755705535126No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA19910.17174616072714788No Hit
CTTACAGTACCCTGTGGACGTTCGGCCACGGGACCAAGGTGGTAATCAAA18520.15975584614097332No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18340.15820314353269174No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18150.15656417966839448No Hit
ACTTACTACTGTCAACAGACTTACAGTACCCTGTGGACGTTCGGCCACGGGACCAAGGTGGTAATCAAACGAA18100.15613287338831627No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18020.1554427833401911No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17570.1515610268194871No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17440.15043963049128373No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT16790.14483264885026684No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16600.1431936849859696No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16380.14129593735362544No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAATCTGTCCCAGATCCACTGGCA16330.14086463107354721No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCTCTCTCA15990.13793174836901528No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15950.1375867033449527No Hit
GATTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTACC15720.13560269445659293No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15440.13318737928815488No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGA15410.13292859552010794No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15270.1317209379358889No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15010.12947814527948218No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14940.12887431648737263No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14630.12620021755088767No Hit
GTTCAGTGCCAGTGGATCTGGGACAGATTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA14440.12456125368659043No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14150.12205967726213675No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14130.12188715475010545No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT14100.12162837098205852No Hit
GTGCCAGTGGATCTGGGACAGATTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTA13850.11947183958166742No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13800.11904053330158919No Hit
GAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC13660.11783287571737018No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13430.11584886682901034No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTACCCTGTGGACGTTCGGCCACGGGA12910.11136328151619683No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12890.11119075900416556No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12840.11075945272408733No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12770.11015562393197782No Hit
GTGGGGTCCCATCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCTCT12530.10808535378760235No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12270.10584256113119561No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12220.10541125485111738No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12120.10454864229096095No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12110.1044623810349453No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12080.10420359726689837No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12010.10359976847478884No Hit
GTGTATTACTGTGCGAGAGCGACGGTTGGCGGATTTATAAGTGGGGCCAG11980.10334098470674191No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11940.10299593968267934No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11880.10247837214658548No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11830.10204706586650725No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11800.10178828209846033No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG11760.10144323707439774No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11700.10092566953830387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG150.002522671867.817435
TAGCGCT1500.047.62217329
CTAGCGC1450.046.88390728
TCGTATG1200.043.2212340
GTATCAA32950.042.294021
CGTATGC1350.041.04693641
GCCGTCT1300.040.2435146
ACTAGCG1750.038.81812727
GACTAGC1750.038.7946626
CGCTCAT1450.037.68312532
TATCAAC37700.036.6054422
GTGGTAT2900.036.245681
ACGAGAC1800.035.7987122
GACGGCT400.002225449535.6012358
ATCAACG40150.034.5406383
TCAACGC40250.034.3720634
AGACTAG2000.033.93506625
CAACGCA41200.033.5795065
AACGCAG42050.033.0620046
ATGCCGT1800.032.84677544
Produced by FastQC (version 0.11.5)