FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159269
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51200.441657630800099No Hit
GGGTACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAAT38170.32925921421171445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35010.30200065731077086No Hit
ACAGTACCCTGTGGACGTTCGGCCACGGGACCAAGGTGGTAATCAAACGA34570.2982051620460825No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCT32550.28078038833092234No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32360.27914142446662504No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29750.2566272366465419No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29570.2550745340382603No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27960.24118647181974157No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27580.2379085440911471No Hit
GGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG26640.22979998602567653No Hit
GACTTACAGTACCCTGTGGACGTTCGGCCACGGGACCAAGGTGGTAATCAAACGAACTGTGGCTGCACCAT26260.22652205829708205No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAAA26260.22652205829708205No Hit
GTACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAAT24130.2081484107657498No Hit
GGACCAAGGTGGTAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC23500.2027139516367642No Hit
CCTTGGTCCCGTGGCCGAACGTCCACAGGGTACTGTAAGTCTGTTGACAG23320.2011612490284826No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCATTAGCAG23090.1991772401401228No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22370.1929664297069964No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22210.19158624961074608No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21950.18934345695433935No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21300.18373647531332243No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20600.17769818739222734No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20390.17588670101589882No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAATCTGTC20250.17467904343167978No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20140.17373016961550772No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGCTGCTAATGCTCTGAC19790.17071102565496016No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19690.1698484130948037No Hit
GTGCAGCCACAGTTCGTTTGATTACCACCTTGGTCCCGTGGCCGAACGTC19660.16958962932675678No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19550.1686407555105847No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19320.16665674662222485No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTACCACCTTGGTCCCGTGGCCGAACGTCCACAGGGTA18540.1599283686530046No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18490.15949706237292638No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18460.15923827860487944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18340.15820314353269174No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA18190.15690922469245708No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18040.15561530585222239No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17920.15458017078003466No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17760.15319999068378437No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17380.14992206295518987No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT16360.14112341484159413No Hit
ACTTACTACTGTCAACAGACTTACAGTACCCTGTGGACGTTCGGCCACGGGACCAAGGTGGTAATCAAACGAA16330.14086463107354721No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAATCTGTCCCAGATCCACTGGCA15900.1371553970648745No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15810.1363790457607337No Hit
CTTACAGTACCCTGTGGACGTTCGGCCACGGGACCAAGGTGGTAATCAAA15760.13594773948065547No Hit
GTGGGGTCCCATCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCTCT15720.13560269445659293No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGA15570.13430877561635823No Hit
GATTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTACC15260.13163467667987327No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14950.1289605777433883No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCTCTCTCA14920.12870179397534137No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14740.12714909136705976No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14430.12447499243057479No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14340.12369864112643399No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14190.12240472228619932No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14030.12102454218994901No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT13880.11973062334971435No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13780.11886801078955792No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13600.1173153081812763No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13490.11636643436510422No Hit
GTGCCAGTGGATCTGGGACAGATTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTA13470.11619391185307293No Hit
GAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC13400.11559008306096341No Hit
GTTCAGTGCCAGTGGATCTGGGACAGATTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA13040.11248467784440022No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12910.11136328151619683No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12690.10946553388385266No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12630.1089479663477588No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12350.10653265117932076No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTACCCTGTGGACGTTCGGCCACGGGA12010.10359976847478884No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11890.10256463340260111No Hit
GTGTATTACTGTGCGAGAGCGACGGTTGGCGGATTTATAAGTGGGGCCAG11830.10204706586650725No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11810.10187454335447597No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11600.10006305697814744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTA150.002284431469.5354143
GTGGTAT3650.042.690651
GTATCAA35250.038.4386981
TACTGAT11650.034.2723370
TATCAAC39250.034.0692562
ATCAACG40250.033.2070473
TCAACGC40550.032.7888264
CAACGCA40750.032.5448725
AACGCAG41300.032.1114656
ATACACG6700.031.3069674
TGCGTTA553.334206E-431.23859437
ACGTGTC6800.030.8161363
ACGCAGA43750.030.3905537
GACGTGT6900.030.27745262
CGCAGAG44300.030.2423538
GTAATAC6850.030.165261
TACACGG6750.030.0726415
AGAGTAC44750.029.93307511
AGACGTG7200.029.92571861
GCAGAGT45550.029.3381589
Produced by FastQC (version 0.11.5)