FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070179
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30130.2815416860170121No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26380.24650081902186458No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20560.19211739344539558No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA19210.17950268132714248No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18590.17370925798394474No Hit
CAGTAATACATAGCCGTGTCTTCAGCTCTCAGACTGCGCATTTGAAGATA18070.16885025776061763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17760.16595354608901874No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17650.1649256806571611No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16300.152310968538908No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16230.15165687235499856No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15460.14446181433199493No Hit
GTAGACACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA14890.1391356025487325No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14500.13549135238123716No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13990.1307257944698971No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13780.12876350591816882No Hit
GTATTACTGTGTGCGCGTGGGTGATAGTTATGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC13540.1265208904304794No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13330.12455860187875112No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13150.12287664026298403No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13080.12222254407907462No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGACTTCACACTGGA12800.11960615934343694No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12270.11465371680812275No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAGTCAGTGCCTGACCCACTGCCG12230.11427994756017451No Hit
GTGTCTACTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC12210.1140930629362004No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT12060.11269142825639449No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12020.11231765900844624No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11890.11110290895261446No Hit
GTCTGGGACCCCAGAGTCCCGCTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGATGAAC11760.10988815889678269No Hit
CGTATACAGTGATGGAAACAGCTACTTGATTTGGGTTCATCAGAGGCCAG11690.10923406271287327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11690.10923406271287327No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11640.10876685115293797No Hit
CTCGTATACAGTGATGGAAACAGCTACTTGATTTGGGTTCATCAGAGGCC11260.10521604329742967No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11160.10428162017755908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)