FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070179
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29000.2709827047624743No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21680.2025829323879463No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20290.18959445102174496No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20050.18735183553405552No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18200.1700650078164494No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA17810.16642075764895406No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16950.15838471881806687No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16200.15137654541903736No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15800.14763885293955498No Hit
CAGTAATACATAGCCGTGTCTTCAGCTCTCAGACTGCGCATTTGAAGATA15770.1473585260035938No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15730.14698475675564554No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15600.14577000669981377No Hit
GTAGACACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA15310.14306017965218903No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14980.13997658335661603No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13700.12801596742227236No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13560.12670777505445352No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13160.1229700825749711No Hit
GTGTCTACTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC12910.1206340247752946No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12610.1178307554156828No Hit
GTCTGGGACCCCAGAGTCCCGCTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGATGAAC12320.11512092836805805No Hit
GTATTACTGTGTGCGCGTGGGTGATAGTTATGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC12280.11474715912010983No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAGTCAGTGCCTGACCCACTGCCG12120.11325208212831686No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGACTTCACACTGGA12080.11287831288036862No Hit
CTCGTATACAGTGATGGAAACAGCTACTTGATTTGGGTTCATCAGAGGCC12000.11213077438447212No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11940.11157012051254978No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11690.10923406271287327No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11380.1063373510412744No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11150.10418817786557201No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10720.10017015845012844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.038.6787761
GTATCAA31350.036.4712031
GTGGTAT4550.035.3722841
TGGTATC4600.033.525282
ATCAACG38050.029.8687763
TATCAAC38450.029.7324312
TCAACGC38600.029.3563334
AACGCAG38950.029.2797376
GTAGTTT3001.8189894E-1229.0023970
CAACGCA39100.028.986355
CGCACTT605.349983E-428.81562841
ACACCGA704.6657042E-528.750736
ACGCAGA40700.027.943597
AGAGTAC40850.027.94139911
CGCAGAG40950.027.7820858
CAGAGTA41350.027.68083810
GCAGAGT42000.027.1712539
GGGATCG2557.0776878E-926.24650870
TACACCG1251.6649756E-724.1427125
CAGTTCG11650.024.12876170
Produced by FastQC (version 0.11.5)