FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651899
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22840.3503610221828842No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18390.28209891409558846No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15030.2305571875397876No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14250.21859214387504813No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12940.19849700643811388No Hit
GTCTGGGACAGACTTCATTCTCACCATCAGCAGCCTAGAGCCTGAAGATT12690.19466205654556917No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12370.18975332068311196No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11530.17686788904416176No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11200.17180575518600275No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10780.16536303936652763No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10480.16076109949547399No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9720.1491028518221381No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9700.14879605583073452No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA9250.14189314602415404No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTTCTGTCAGCAACATACCAACTTGGCCGCAAGTTTCGGCCCTGGGA9180.14081936005424153No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9020.1383649921230129No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8810.13514363421327535No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8790.1348368382218718No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8710.13360965425625748No Hit
GACTTCATTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA8700.1334562562605557No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTCTT8370.1283941224023967No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8340.12793392841529133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8340.12793392841529133No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8310.12747373442818596No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8310.12747373442818596No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8210.12593975447116806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8050.12348538653993947No Hit
GTGCAGCCACAGTTCGTTTGATATGCACTTTGGTCCCAGGGCCGAAACTT8040.12333198854423769No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATGCACTTTGGTCC7800.11965043664739478No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7560.11596888475055185No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGAAACTTAGCCTGGTA7340.11259412884511251No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGAAACTTAGCCTGGTACCAACAGAAACCTGGCCAGACTCCCAGGCT7290.11182713886660356No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7050.10814558696976065No Hit
GAATGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA6940.10645820901704099No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT6860.10523102505142667No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6840.1049242290600231No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6790.10415723908151417No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6620.10154947315458376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG352.6704367E-547.9561732
CTAACGC405.896252E-541.93593
GTATCAA24050.038.1262051
TATCAAC27500.032.9589732
GGTATCA10950.032.207071
ATCAACG28450.031.8388543
CGGTACC553.0735333E-431.6725245
TCAACGC28650.031.3823954
CAACGCA28850.031.167235
AACGGCC553.4662735E-431.02972637
TAACGGC553.5013212E-430.9765336
AACGCAG29200.030.9085546
AGAGTAC30750.029.23696511
CGCAGAG30650.029.227398
ACGCAGA30800.029.1939817
TTAACGG605.8653037E-428.3642135
GATCTAG606.0520123E-428.21268331
GAGTTTA4000.027.7095811
ATGTACC855.502492E-627.6262411
GCAGAGT32700.027.3950929
Produced by FastQC (version 0.11.5)