FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849526
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAACACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA34370.40457855321673497No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34140.4018711610945398No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29560.34795874405256577No Hit
GTGTTGCTCCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC26330.30993754164086795No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTGTTGCTCCTTGTATATGAGCTGCAGTAATAAT26160.30793642572446284No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26110.3073478622196378No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTGTTGCTCCTTGTATATGAGCTG25250.29722456993664703No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24460.28792526656041134No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC23580.27756654887549054No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG23200.27309346623882025No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22500.2648535771712696No Hit
GTAATAGACAGCCGTGTCGTCAACTCTCAGGCTGTTCATTTGCACATACA22350.2630878866567945No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21150.24896236254099346No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC21020.24743209742844832No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20270.2386036448560727No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19640.23118774469527711No Hit
GTCTATTACTGTGCGATCGGGTTGATGAAGTGGCTGAGTCCCGACTACTGGGGCCAGGGAACCCTGGTCACCGT19540.23001061768562706No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT19350.22777407636729186No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19160.2255375350489567No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT18670.2197696127016713No Hit
GATTATTACTGCAGCTCATATACAAGGAGCAACACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT17980.2116474363350857No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17930.21105887283026062No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17280.2034075472675351No Hit
ACACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC17090.2011710059491999No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17050.20070015514533987No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16170.1903414374604191No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG15880.18692776913243386No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15540.18292553729962355No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15350.18068899598128838No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACTATGTCTCCTGGTACCAACAGCGCCCAGGCAAAGC15240.1793941562706733No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15200.17892330546681326No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15030.17692218955040812No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14840.17468564823207294No Hit
GTACCAACAGCGCCCAGGCAAAGCCCCCGAACTCATGATTTATGATGTCA14750.17362623392338788No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACTATGTCTCCTGGTACCAACAGCG14690.1729199577175978No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14610.17197825610987774No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGTGAC14430.1698594274925076No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14410.16962400209057757No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14280.16809373697803245No Hit
GTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG14140.16644575916452234No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14130.16632804646355734No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13980.1645623559490822No Hit
CCACAGTGTTGCTCCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC13950.16420921784618717No Hit
CTGTTGGTACCAGGAGACATAGTTATAAGTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA13730.161619538424957No Hit
GGCAAAGCCCCCGAACTCATGATTTATGATGTCAGTAATCGGCCCTCAGG13420.1579704446950417No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13340.15702874308732162No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13300.1565578922834616No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13230.15573390337670653No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12840.15114310803907122No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12620.14855342861784102No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12590.148200290514946No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12260.1443157713831007No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12130.14278550627055558No Hit
GGTACCAACAGCGCCCAGGCAAAGCCCCCGAACTCATGATTTATGATGTC11940.1405489649522204No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11860.13960726334450035No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11790.13878327443774527No Hit
GTTGGTACCAGGAGACATAGTTATAAGTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTC11530.13572274421265507No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11500.13536960610976No Hit
GAATACCACAGTGTTGCTCCTTGTATATGAGCTGCAGTAATAATCAGCCT11320.1332507774923899No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11240.1323090758846698No Hit
GTTATAAGTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG10680.12571716463062932No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10550.12418689951808419No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10540.1240691868171192No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC10510.12371604871422416No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC10480.12336291061132913No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10480.12336291061132913No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTGTTGCTCC10310.12136179469492399No Hit
CATATACAAGGAGCAACACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCC10270.12089094389106396No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA10260.12077323119009895No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10170.11971381688141386No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC10110.11900754067562382No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTCGGGGGCTTTGCCTGGGCGCTGTTGGTACCAGGAGA9790.11524073424474354No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATC9780.11512302154377853No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9720.11441674533798848No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9690.11406360723509344No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9670.11382818183316343No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9640.1134750437302684No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9630.1133573310293034No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT9630.1133573310293034No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9590.11288648022544337No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG9530.1121802040196533No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC9260.10900196109359808No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9120.10735398328008795No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9120.10735398328008795No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTGTTG9090.10700084517719292No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8760.10311632604534764No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8700.1024100498395576No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT8540.10052664662411745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)