FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849526
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34470.40575568022638503No Hit
GAGCAACACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA32770.3857445210623336No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27300.3213556736344738No Hit
GTGTTGCTCCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC27180.3199431212228937No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26770.31511690048332835No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC25680.30228621607814243No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25230.29698914453471703No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTGTTGCTCCTTGTATATGAGCTG25090.2953411667212069No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTGTTGCTCCTTGTATATGAGCTGCAGTAATAAT24030.282863620418916No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23410.2755654329590854No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22250.2619107596471444No Hit
GTAATAGACAGCCGTGTCGTCAACTCTCAGGCTGTTCATTTGCACATACA20080.2363671035377375No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19750.2324825844058922No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT19620.2309523192933471No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC19560.23024604308755706No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19370.2280095017692219No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19140.2253021096470267No Hit
GTCTATTACTGTGCGATCGGGTTGATGAAGTGGCTGAGTCCCGACTACTGGGGCCAGGGAACCCTGGTCACCGT18890.22235929212290148No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18130.21341312684956082No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18130.21341312684956082No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17920.21094116012929562No Hit
GATTATTACTGCAGCTCATATACAAGGAGCAACACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT17800.20952860771771553No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT17360.20434924887525516No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16850.1983459011260397No Hit
ACACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC16330.19222484067585924No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16220.19093000096524415No Hit
GTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG16100.18951744855366406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16100.18951744855366406No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACTATGTCTCCTGGTACCAACAGCGCCCAGGCAAAGC15940.18763404533822392No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACTATGTCTCCTGGTACCAACAGCG15490.18233697379479852No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14960.17609820064365306No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14830.17456793553110794No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14630.17221368151180777No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14580.1716251180069827No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14490.17056570369829763No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14400.16950628938961254No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14280.16809373697803245No Hit
GTACCAACAGCGCCCAGGCAAAGCCCCCGAACTCATGATTTATGATGTCA14060.16550405755680228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13970.1644446432481172No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13850.16303209083653708No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13310.15667560498442662No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13300.1565578922834616No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13210.1554984779747765No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13200.15538076527381153No Hit
CCACAGTGTTGCTCCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC12820.1509076826371412No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12820.1509076826371412No Hit
CTGTTGGTACCAGGAGACATAGTTATAAGTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA12700.14949513022556107No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12510.14725858890722593No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12450.14655231270143584No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12300.14478662218696073No Hit
GGCAAAGCCCCCGAACTCATGATTTATGATGTCAGTAATCGGCCCTCAGG12210.14372720787827564No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11970.14090210305511544No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11910.14019582684932538No Hit
GTTGGTACCAGGAGACATAGTTATAAGTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTC11890.13996040144739538No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGTGAC11840.13937183794257033No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11490.13525189340879504No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11420.13442790450203995No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11210.13195593778177478No Hit
GGTACCAACAGCGCCCAGGCAAAGCCCCCGAACTCATGATTTATGATGTC10910.12842455675282452No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10700.12595259003255935No Hit
GAATACCACAGTGTTGCTCCTTGTATATGAGCTGCAGTAATAATCAGCCT10480.12336291061132913No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10380.12218578360167906No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC10370.12206807090071403No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10160.11959610418044886No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG10140.11936067877851884No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA10130.11924296607755383No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10050.11830126446983377No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10040.11818355176886874No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10030.11806583906790376No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10000.11771270096500872No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC9920.11677099935728866No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9900.11653557395535864No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTCGGGGGCTTTGCCTGGGCGCTGTTGGTACCAGGAGA9640.1134750437302684No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC9630.1133573310293034No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9370.11029680080421318No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTGTTG9330.10982595000035313No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT9320.10970823729938813No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9100.10711855787815794No Hit
CATATACAAGGAGCAACACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCC9040.1064122816723679No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8960.10547058006464781No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC8940.1052351546627178No Hit
GTTATAAGTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG8790.10346946414824267No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC8780.10335175144727765No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8590.10111521012894249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGA200.00736100851.7452741
GTATCAA11100.042.7612231
GGTATCA4200.042.2777941
TAGGTAG350.001311554739.6466745
GCTTAGA350.001413304739.04865311
TCTGGAG6900.037.9920170
GGATGGG8400.037.77776770
TATCAAC12750.037.754172
ATCAACG12900.037.043983
TCAACGC13050.036.356634
CAACGCA13150.036.0843965
AACGCAG13350.035.812176
TGGTATC1400.034.1394122
TACATGC501.9795175E-434.139412
ACGCAGA14050.034.035957
CGCAGAG14000.033.6754958
AGAGTAC14200.033.205111
TCGTCAA4900.032.75753417
GCAGAGT14550.032.404459
CGTCAAC4850.032.3987218
Produced by FastQC (version 0.11.5)