FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065320
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41610.3905868659182218No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34520.3240340930424661No Hit
TCTATGATGTGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTAGGTCTGGGACAGACTTCAGT33820.3174632974129839No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30530.28658055795441745No Hit
GTCTGGGACAGACTTCAGTCTCATCATCAGCAGCCTGGAGCCTGAGGATT30380.28517253031952844No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA28550.267994593173882No Hit
GTCCCAGACCTACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT28050.2633011677242519No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAG27760.2605789809634664No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26690.25053505050125785No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGTCAACACTCTGA26530.24903315435737616No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24420.22922689895993692No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAGCTACTTAGCCTGGTA24020.2254721586002328No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT23410.219746179551684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22390.21017159163443846No Hit
GTAATATAGAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGACTCA22000.20651071978372695No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21440.2012540832801412No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20590.19327526001576992No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20380.19130402132692526No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19460.18266811849960574No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19460.18266811849960574No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18890.17731761348702738No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18760.17609732287012353No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18530.17393834716329368No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18160.17046521233056736No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACT17610.16530244433597416No Hit
GTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTAGGTCTGGGACAGACTTCAGTCTCATCATCAG17560.16483310179101116No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17390.1632373371381369No Hit
CTATATTACTGTGCGAAAGAAGGTTTTGGTGACGGTTCGGGGAGTTATCC17320.16258025757518868No Hit
CATCTATGATGTGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTG17270.16211091503022568No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGC16140.1515037735140615No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16060.15075282544212068No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16020.15037735140615027No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15580.14624713701047573No Hit
GCCCTGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTG15560.14605939999249054No Hit
GAGTGTTGACAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTGTC15490.14540232042954232No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC15440.1449329778845793No Hit
GCGTAGCGTCTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15120.141929185596816No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14650.13751736567416362No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14560.1366725490932302No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14210.13338715127848907No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14090.13226072917057785No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13690.12850598881087372No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13180.12371869485225095No Hit
CTGCTACTCTGGCTCCCAGATGCCACCGGAGAAGTTGTGTTGACACAGTC12910.1211842451094507No Hit
ACGCTACGCTGCTGACAATAATAAAATGCAAAATCCTCAGGCTCCAGGCTGCTGATGATGAGACTGAAGTCTGTC12730.11949461194758382No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12470.11705403071377615No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12370.1161153456238501No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12230.11480118649795365No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12120.11376863289903504No Hit
ATATTACTGTGCGAAAGAAGGTTTTGGTGACGGTTCGGGGAGTTATCCCTACTTTGACGACTGGGGCCAGGGAAC11930.11198513122817556No Hit
TTATTATTGTCAGCAGCGTAGCGTCTGGCCGCTCACTTTCGGCGGAGGGA11910.11179739421019036No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11870.11142192017421997No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11790.11067097210227914No Hit
GAGTACGGGAGAGCCCTGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT11700.1098261555213457No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTGTCCAACAGGGCCACTGGCATCCCAGCCAGGTT11670.10954454999436788No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11570.10860586490444187No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11480.10776104832350843No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11280.10588367814365637No Hit
GCCAGACGCTACGCTGCTGACAATAATAAAATGCAAAATCCTCAGGCTCCAGGCTGCTGATGATGAGACTGAAGT11150.10466338752675253No Hit
CTCCCAGGCTCCTCATCTATGATGTGTCCAACAGGGCCACTGGCATCCCA10980.10306762287387829No Hit
CAGTAATATAGAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGACT10880.10212893778395224No Hit
GCCTGGAGCCTGAGGATTTTGCATTTTATTATTGTCAGCAGCGTAGCGTCTGGCCGCTCACTTTCGGCGGAGGGA10820.10156572672999661No Hit
GTCAGAGTGTTGACAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAG10810.10147185822100402No Hit
CTGTTGGACACATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTG10790.10128412120301881No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG10780.1011902526940262No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10740.10081477865805578No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10700.10043930462208539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)