FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065320
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40120.3766004580783239No Hit
GTCTGGGACAGACTTCAGTCTCATCATCAGCAGCCTGGAGCCTGAGGATT30130.2828258175947133No Hit
TCTATGATGTGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTAGGTCTGGGACAGACTTCAGT29780.2795404197799722No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29660.278413997672061No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29190.2740021777494086No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA26190.24584162505162768No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25120.23579769458941915No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAG24870.23345098186460406No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24730.23213682273870762No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAGCTACTTAGCCTGGTA24350.2285698193969887No Hit
GTCCCAGACCTACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT24170.22688018623512185No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGTCAACACTCTGA22600.21214283032328315No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT22380.21007772312544587No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22250.20885743250854205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21290.19984605564525212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21250.19947058160928172No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19930.18707993842225812No Hit
GTAATATAGAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGACTCA19430.18238651297262795No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18610.1746892952352345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17960.1685878421507153No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACT17740.166522734952878No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17570.16492697030000375No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17300.16239252055720346No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17280.16220478353921827No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17200.16145383546727746No Hit
GTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTAGGTCTGGGACAGACTTCAGTCTCATCATCAG16680.15657267299966207No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16540.15525851387376563No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16240.15244245860398753No Hit
CATCTATGATGTGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTG16170.1517853790410393No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGC15780.1481245071903278No Hit
CTATATTACTGTGCGAAAGAAGGTTTTGGTGACGGTTCGGGGAGTTATCC15690.14727969060939436No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15660.14699808508241655No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15640.14681034806443136No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15420.14474524086659407No Hit
GCCCTGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTG15050.14127210603386778No Hit
GAGTGTTGACAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTGTC14900.13986407839897871No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14470.13582773251229677No Hit
CTGCTACTCTGGCTCCCAGATGCCACCGGAGAAGTTGTGTTGACACAGTC14190.13319941426050388No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14120.13254233469755566No Hit
GCGTAGCGTCTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13620.1278489092479255No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC13440.12615927608605865No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13210.12400030037922878No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12850.12062103405549507No Hit
ACGCTACGCTGCTGACAATAATAAAATGCAAAATCCTCAGGCTCCAGGCTGCTGATGATGAGACTGAAGTCTGTC12400.11639695115082793No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12360.1160214771148575No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12360.1160214771148575No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12290.11536439755190928No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12260.11508279202493148No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12220.11470731798896106No Hit
GAGTACGGGAGAGCCCTGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT11590.10879360192242707No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11210.10522659858070815No Hit
ATATTACTGTGCGAAAGAAGGTTTTGGTGACGGTTCGGGGAGTTATCCCTACTTTGACGACTGGGGCCAGGGAAC11160.10475725603574512No Hit
GCCAGACGCTACGCTGCTGACAATAATAAAATGCAAAATCCTCAGGCTCCAGGCTGCTGATGATGAGACTGAAGT11030.10353696541884128No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11010.10334922840085609No Hit
GCCTGGAGCCTGAGGATTTTGCATTTTATTATTGTCAGCAGCGTAGCGTCTGGCCGCTCACTTTCGGCGGAGGGA11000.10325535989186348No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10990.10316149138287088No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10790.10128412120301881No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10740.10081477865805578No Hit
TTATTATTGTCAGCAGCGTAGCGTCTGGCCGCTCACTTTCGGCGGAGGGA10740.10081477865805578No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10720.1006270416400706No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10710.10053317313107799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)