FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481648
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC75170.5073404749306178No Hit
CTCTAAAGTAGACGCCTTATAGATCAGCAGTTTAGGGGCTTTCCCTGGTT69960.47217692731336997No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62900.42452728313337584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62030.41865544312819236No Hit
ACTTTAGAGAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC58990.39813774931697676No Hit
AGTCAGGACACAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC54160.3655389134261309No Hit
GTCAGGACACAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC50770.34265898513007137No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA50290.3394193492651426No Hit
GTATAAAGATTATTTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT48010.3240310789067309No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48000.3239635864928782No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47690.321871321663445No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44950.3033784002678099No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44100.29764154509033186No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA42790.2888000388756304No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42110.2842105547336479No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTGCTGATCTATAAGGCGT41830.2823207671457728No Hit
TCTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC40070.2704421023077006No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39720.2680798678228567No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39430.2661225878211289No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36970.24951945401336892No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35970.24277021262810058No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35170.23737081951988598No Hit
GTCTGCAACTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGA34720.23433366089651522No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34090.2300816388237962No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33180.22393982916320207No Hit
CCTTCCACCCTGTCTGCAACTGTAGGAGACAGAGTCACCATCACTTGCCG32050.2163131863978489No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCA30940.20882152846020108No Hit
GGACACAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30130.20335464293813377No Hit
CCTTATAGATCAGCAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTTA29910.2018698098333747No Hit
GACCCAGTCAGGACACAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGG29260.19748280293295034No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29170.1968753712082762No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28970.19552552293122255No Hit
CTAATACTCTTACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGT28880.1949180912065484No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA28790.19431065948187423No Hit
GCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28580.1928933187909679No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27280.1841193049901191No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26760.1806096994697796No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA26690.18013725257281082No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC26640.1797997905035474No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC24950.16839357256244397No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24720.16684124704383227No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24230.1635341187650508No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23380.15779726358757273No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCCAAATAAT23260.15698735462134056No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23020.15536753668887618No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22850.15422016565338056No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22730.15341025668714836No Hit
CCCCTAAACTGCTGATCTATAAGGCGTCTACTTTAGAGAGTGGGGTCCCA22530.1520604084100947No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22500.15185793116853666No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22500.15185793116853666No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22040.14875328013131323No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21920.14794337116508105No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21740.14672850771573273No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21560.14551364426638444No Hit
GTTGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC21380.14429878081703618No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21260.14348887185080397No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21220.14321890219539324No Hit
TCTATAAGGCGTCTACTTTAGAGAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT21170.14288144012612983No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG21070.14220651598760298No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20880.140924160124402No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20820.14051920564128592No Hit
GTCTCCTTCCACCCTGTCTGCAACTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGAGTATTAGTAG20570.13883189529496884No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20170.13613219874086152No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20100.13565975184389273No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19850.13397244149757567No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19620.13242011597896397No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19530.13181268425428982No Hit
GTCTCCTACAGTTGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG19230.12978791183870933No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA19100.12891051045862445No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18960.1279656166646869No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18740.12648078355992787No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18690.12614332149066446No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18480.12472598079975811No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18470.12465848838590543No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18410.12425353390278933No Hit
ACCTTGATGGGACCCCACTCTCTAAAGTAGACGCCTTATAGATCAGCAGTTTAGGGGCTTTCCCTGGTTTCTG18310.12357860976426249No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17960.1212163752794186No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17830.12033897389933372No Hit
ACTTATTACTGCCAACAGTATAAAGATTATTTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17360.11716683044825761No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC17340.11703184562055224No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTGCTGATCTATAAGGCGTCTA16940.11433214906644493No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16830.11358973251406543No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16740.11298230078939128No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16390.11062006630454736No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT16320.1101476194075786No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16310.1100801269937259No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16260.1097426649244625No Hit
ATCTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT16220.10947269526905175No Hit
GCTGATACCAGGCCAACCAGCTACTAATACTCTTACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGTTG16130.10886526354437762No Hit
CAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15840.10690798354264981No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCACTCTCTAAAGTAGA15700.10596308974871224No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAGATTATTTGGGGACGTT15480.10447825664395322No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG15260.1029934235391942No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15170.10238599181452004No Hit
GTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCACTCTCTAAAGT15160.10231849940066737No Hit
ATTTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15090.10184605250369858No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT14950.10090115870976103No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTGCTGATCTATAAGGCGTCTACTTTAGAGAGTGGGGTCCCAT14940.10083366629590833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)