FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481648
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAGTAGACGCCTTATAGATCAGCAGTTTAGGGGCTTTCCCTGGTT65380.44126540176884116No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63640.4295217217584743No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC60450.4079916417394685No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60420.4077891644979104No Hit
ACTTTAGAGAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC57910.39084856862088696No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52080.35150049134477285No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50020.3375970540911202No Hit
AGTCAGGACACAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC48670.32848557822100793No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA47820.3227487230435299No Hit
GTATAAAGATTATTTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT47140.31815923890154746No Hit
GTCAGGACACAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC46540.31410969407038647No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42880.28940747060030453No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA41230.2782712223146118No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40890.2759764802436206No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG40760.27509907886353574No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT39770.26841732989212014No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTGCTGATCTATAAGGCGT39330.2654476636826021No Hit
TCTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC37760.25485135470773085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37160.25080180987656986No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36510.24641480297614549No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35960.2427027202142479No Hit
GTCTGCAACTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGA35130.23710084986447522No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35090.2368308802090645No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34520.23298381261946158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33190.22400732157705472No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31000.2092264829433172No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30910.20861905121864305No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCA30380.20504195328445085No Hit
GGACACAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29660.20018249948705766No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29530.19930509810697278No Hit
GACCCAGTCAGGACACAGCGTGGACATGAGGGTCCCCGCTCAGCTCCTGG29360.19815772707147716No Hit
CCTTCCACCCTGTCTGCAACTGTAGGAGACAGAGTCACCATCACTTGCCG29130.19660540155286543No Hit
CTAATACTCTTACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGT28370.19147597810006156No Hit
GCGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28130.18985616016759715No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG27840.18789888016586934No Hit
CCTTATAGATCAGCAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTTA27640.1865490318888157No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA27600.18627906223340496No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27420.1850641987840567No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27230.1837818429208557No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC26800.18087966912519032No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA25600.17278057946286837No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25100.16940595877023423No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC24830.16758366359621177No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24040.16225176290184984No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24020.16211677807414449No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23660.15968705117544787No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23500.15860717255380494No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23050.15557001393043424No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22910.15462512013649665No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCCAAATAAT22730.15341025668714836No Hit
CCCCTAAACTGCTGATCTATAAGGCGTCTACTTTAGAGAGTGGGGTCCCA22290.15044059047763034No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22120.1492932194421347No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22040.14875328013131323No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21870.14760590909581764No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21800.14713346219884885No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21350.14409630357547812No Hit
GTCTCCTTCCACCCTGTCTGCAACTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGAGTATTAGTAG20940.14132911460751812No Hit
GTTGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC20820.14051920564128592No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20640.13930434219193763No Hit
GTCTCCTACAGTTGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG19640.13255510080666932No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19540.1318801766681425No Hit
TCTATAAGGCGTCTACTTTAGAGAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT19450.13127274494346836No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19040.12850555597550833No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18830.127088215284602No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18810.12695323045689666No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA18590.12546839735213763No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18530.12506344286902152No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18320.12364610217811518No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18230.12303867045344104No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18110.12222876148720885No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17810.12020398907162835No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17720.11959655734695421No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17330.11696435320669957No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17170.11588447458505664No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTGCTGATCTATAAGGCGTCTA16680.11257734630627518No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16650.11237486906471714No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG16560.11176743734004299No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16530.11156496009848493No Hit
ACCTTGATGGGACCCCACTCTCTAAAGTAGACGCCTTATAGATCAGCAGTTTAGGGGCTTTCCCTGGTTTCTG16480.11122749802922151No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC16240.10960768009675712No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16230.10954018768290444No Hit
GTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCACTCTCTAAAGT16150.10900024837208297No Hit
ACTTATTACTGCCAACAGTATAAAGATTATTTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15960.107717892508882No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15810.10670550630109175No Hit
ATCTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT15710.10603058216256493No Hit
ATTTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15690.10589559733485956No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15570.10508568836862736No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAGATTATTTGGGGACGTT15290.10319590078075225No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15270.10306091595304687No Hit
GCTGATACCAGGCCAACCAGCTACTAATACTCTTACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGTTG15200.1025884690560781No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15070.10171106767599322No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG15050.10157608284828785No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15050.10157608284828785No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCACTCTCTAAAGTAGA14850.10022623457123418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA5950.039.55752670
GTATCAA26700.035.7402421
GTGGTAT4200.035.20091
TACCGCT400.002903977233.7215427
TGTTAGA25450.032.09677570
AATGGTA8200.031.23597370
TATCAAC30700.030.6384942
TCAACGC31700.030.1997994
ATCAACG31900.030.1157573
CAACGCA32050.029.7672085
AACGCAG32350.029.6009966
GATTCAC2700.028.6229551
ACGCAGA33900.028.2609147
AGAGTAC34050.027.95974511
CGCAGAG34550.027.735798
GCAGAGT34900.027.5586369
CAGAGTA34700.027.5310910
GTATTGC500.00879914826.880691
GAGTTTA4700.026.4517421
GCATCGT659.3008444E-426.21499435
Produced by FastQC (version 0.11.5)