FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131190
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAACTCAGGCC39930.3529910978703843No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37040.32744278149559314No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG32790.2898717280032532No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30590.27042318266604193No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29420.2600800926457978No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATAGA29110.2573396158028271No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27950.24708492826138845No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTTCGCCCGTCACAAA26070.2304652622459534No Hit
GTAATATGTTGCAACGTCTTCAGGCTGCAGGCCGCTGATGGCGAAAGTAAAATCTGTCCCAGATCCATATCCA25830.22834360275462126No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25220.22295105154748537No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24920.22029897718332023No Hit
ACATATTACTGTCAACAATATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAA24280.21464121853976784No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23900.21128192434515863No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23650.209071862375021No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23610.20871825245979894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23340.2063313855320503No Hit
ATATTACTGTCAACAATATGATAATCTCCCGATCACCTTCGGCCAAGGGA22700.20067362688849796No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22560.19943599218522085No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20350.17989904436920412No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20110.17777738487787198No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19930.17618614025937288No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACATCTATTTAAATTGGTATCAGCAGAAA19490.1722964311919306No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19270.1703515766582095No Hit
CAGTAATATGTTGCAACGTCTTCAGGCTGCAGGCCGCTGATGGCGAAAGTAAAATCTGTCCCAGATCCATATCCA18990.16787630725165534No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGAGATTATCATATTGTTGACAGTAATATGTTGCAACGTCTTCAGG18420.16283736595974152No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18080.15983168168035433No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17970.15885925441349377No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAT17830.15762161971021668No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17310.1530246908123304No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16810.1486045668720551No Hit
GTGTATTACTGTGCAGCAGATCGTCCCCACCGCCGGGTCGACCCCTGGGG16570.14648290738072295No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT15650.13834987933061643No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT15440.13649342727570082No Hit
CGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15410.1362282198392843No Hit
CCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCA15380.1359630124028678No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15370.13587460992406228No Hit
GCCTGAAGACGTTGCAACATATTACTGTCAACAATATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGA15370.13587460992406228No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15340.13560940248764577No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG15080.1333109380387026No Hit
GATTTTACTTTCGCCATCAGCGGCCTGCAGCCTGAAGACGTTGCAACATATTACTGTCAACAATATGATAATCTC14740.1303052537593154No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14650.12950963145006586No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14520.12836039922559428No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14520.12836039922559428No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14040.12411708024293001No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC13920.12305625049726393No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG13770.12173021331518136No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13760.12164181083637585No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13470.11907813895101618No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13390.11837091912057214No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT13350.11801730920535011No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12820.11333197782865832No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGATATGGATCTGGGACAGATTTTA12810.11324357534985281No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12520.11067990346449313No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12400.10961907371882709No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12380.10944226876121606No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTT12260.10838143901554999No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12160.10749741422749494No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG12130.10723220679107844No Hit
GTTGAAGCTCTTTGTGACGGGCGAACTCAGGCCCTGATGGGTGACTTCGC12120.10714380431227293No Hit
AGTCAGGACATTAACATCTATTTAAATTGGTATCAGCAGAAACCAGGGAA12070.10670179191824539No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG12010.10617137704541237No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11970.10581776713019034No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11920.1053757547361628No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11900.10519894977855178No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11890.1051105472997463No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11770.10404971755408023No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11670.10316569276602515No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11650.10298888780841414No Hit
GAGTAATACATACTACGCGGACTCCGTGAAGGGCCGATTCACCGTCTCCA11610.10263527789319213No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11570.1022816679779701No Hit
CATCAAGGTTCAGTGGATATGGATCTGGGACAGATTTTACTTTCGCCATCAGCGGCCTGCAGCCTGAAGACGTTG11560.10219326549916459No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAACTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11550.10210486302035911No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11500.10166285062633157No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11500.10166285062633157No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11490.10157444814752606No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11470.10139764318991505No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11340.10024841096544347No Hit
CTTTGTGACGGGCGAACTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11320.10007160600783245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA150.002490779668.03528628
GTCGTAC301.0079875E-556.5431821
TCTGGAG10900.051.543570
TGCGTAT200.007663648651.22172533
TCACGAT200.007759812851.06041329
CCTAACG200.0078637450.8888662
TACGTAT554.2532448E-449.82918570
CGTATGC1850.048.50955641
TCGTATG1800.047.8469340
GTATGCC2000.044.95018842
ACGAGAC2350.044.7731222
GTGGTAT2450.044.3113941
ATGCCGT2050.043.9806644
CACGAGA2500.042.0811521
ATCTCGT1800.041.905937
TAAGACA2600.039.348831
GTCGTAT350.001368000439.3094339
GCTAGAC350.001463681738.772472
TCCGAGC2850.038.08888213
GTATCAA27050.038.0020371
Produced by FastQC (version 0.11.5)