FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131190
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35830.31674608156012696No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG33420.2954410841680001No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30460.2692739504415704No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAACTCAGGCC29900.26432341162846207No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28890.255394761269106No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28870.25521795631149496No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATAGA26030.23011165233073136No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25830.22834360275462126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTTCGCCCGTCACAAA23690.209425472290243No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23660.2091602648538265No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23570.20836464254457696No Hit
GTAATATGTTGCAACGTCTTCAGGCTGCAGGCCGCTGATGGCGAAAGTAAAATCTGTCCCAGATCCATATCCA23410.20695020288368887No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22960.2029720913374411No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21570.1906841467834758No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21460.18971171951661522No Hit
ACATATTACTGTCAACAATATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAA21420.1893581096013932No Hit
ATATTACTGTCAACAATATGATAATCTCCCGATCACCTTCGGCCAAGGGA21000.18564520549156197No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20410.18042945924203715No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20020.17698176256862244No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTT19390.17141240640387556No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19040.16831831964568286No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACATCTATTTAAATTGGTATCAGCAGAAA18980.16778790477284983No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18710.16540103784510116No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18010.1592128643287158No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGAGATTATCATATTGTTGACAGTAATATGTTGCAACGTCTTCAGG17940.15859404697707724No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17530.1549695453460515No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAT17150.15161025115144228No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17030.1505494214057762No Hit
CAGTAATATGTTGCAACGTCTTCAGGCTGCAGGCCGCTGATGGCGAAAGTAAAATCTGTCCCAGATCCATATCCA16230.14347722310133576No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16140.14268160079208622No Hit
GTGTATTACTGTGCAGCAGATCGTCCCCACCGCCGGGTCGACCCCTGGGG15490.13693543966972835No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15170.13410656034795215No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14970.13233851077184203No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14820.13101247358975945No Hit
GCCTGAAGACGTTGCAACATATTACTGTCAACAATATGATAATCTCCCGATCACCTTCGGCCAAGGGACACGA14670.12968643640767688No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14440.12765317939515025No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT14390.1272111670011227No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14280.12623873973426214No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14250.12597353229784564No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14210.12561992238262362No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG14150.12508950750979056No Hit
GATTTTACTTTCGCCATCAGCGGCCTGCAGCCTGAAGACGTTGCAACATATTACTGTCAACAATATGATAATCTC13830.1222606281880144No Hit
CGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13800.12199542075159786No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13740.12146500587876484No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT13670.12084618852712631No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC13280.11739849185371158No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG13020.11510002740476843No Hit
CCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCA12870.11377399022268583No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12780.1129783679134363No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12770.11288996543463078No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12600.11138712329493718No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12470.11023789107046562No Hit
AGTCAGGACATTAACATCTATTTAAATTGGTATCAGCAGAAACCAGGGAA12300.10873504893077202No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12290.1086466464519665No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG11920.1053757547361628No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11890.1051105472997463No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11820.10449172994810775No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11470.10139764318991505No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGATATGGATCTGGGACAGATTTTA11450.10122083823230403No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11420.10095563079588751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTA400.006309195453.7999470
TCTGGAG8550.049.50013770
GTGGTAT2750.045.5974541
ACGATCA657.727739E-444.1435470
GTCTCTT7750.037.94920770
GTATCAA25000.037.007881
ACAGGTA1400.036.3107151
CGATAAA400.002707988734.2049335
AATGGTA5300.033.8364470
TATCAAC27650.033.192372
CGTTGTC7650.033.1514963
ATCAACG29000.031.7485373
GTTGTCT8050.031.61792264
TCAACGC29250.031.4716194
GACAGGT1950.031.2830751
CAACGCA29750.031.056445
AACGCAG30750.030.156546
TACGTTT450.00506705630.09646815
AAAACCG1703.2680528E-629.53722270
GTGAGCG4950.028.98313170
Produced by FastQC (version 0.11.5)