FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934712
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35340.3780843725126028No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAACTTGTTGCTGTCTGCATATGAGCTGCAGTAATAAT33860.36225061837229006No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAACTTGTTGCTGTCTGCATATGAGCTG33230.3555105743801299No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG31840.34063968366726866No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29060.31089790224154606No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28430.3041578582493859No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT26690.28554249865199116No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA24210.25901026198444016No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22860.24456731057266837No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22190.2373993272794187No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC21740.23258501014216146No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21120.22595195097527368No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20320.21739316495348301No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19790.21172296921404668No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT19410.2076575458536961No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19260.20605277347461037No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18830.20145242598789787No Hit
GATTATTACTGCAGCTCATATGCAGACAGCAACAAGTTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT18490.19781494192863686No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18370.19653112402536824No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18230.19503333647155488No Hit
GTATATTACTGTGCGACAGACCTCAGGCGCGCAGCTGGCCCCGCGTATTATTTTGACTATTGGGGCCAGGGAACC17860.1910748979364767No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17800.1904329889848424No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17710.18947012555739093No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17400.18615359597394704No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17190.183906914643227No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCTGGTACCAACAGCA16200.17331541694126104No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15530.16614743364801138No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAG15440.1651845702205599No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15380.1645426612689256No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15350.16422170679310846No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAACTTGTTGCTGT14710.15737467797567595No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT14590.15609086007240733No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14540.15555593594604541No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14480.15491402699441112No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14160.15149051258569485No Hit
GGTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAG13800.14763905887588905No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13280.14207584796172512No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA13260.14186187831118036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12980.13886630320355361No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT12950.13854534872773647No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12840.13736851564974023No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG12740.1362986673970164No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12650.13533580396956496No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12520.133945001241024No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12470.13341007711466205No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG12430.13298213781357254No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12130.129772593055401No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12100.12945163857958386No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12040.12880972962794957No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12020.1285957599774048No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC11960.1279538510257705No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11840.1266700331225019No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG11770.1259211393455952No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGACAGCAACAAGTTGCTGTTCGGCGGAGGGACCAAGCTGAC11610.1242093821412371No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11420.12217667046106181No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11350.12142777668415511No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11270.12057189808197606No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11190.11971601947979699No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT11160.11939506500397983No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA11150.11928808017870746No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTTAGTAGATATGTCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG11090.11864617122707315No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11030.11800426227543885No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10900.11661345954689786No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10830.1158645657699912No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10790.11543662646890165No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG10720.11468773269199496No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10710.11458074786672258No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10670.11415280856563305No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10650.11393883891508828No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10640.11383185408981591No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT10610.11351089961399874No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10530.11265502101181968No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10490.11222708171073016No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC10480.11212009688545777No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT10300.11019437003055485No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10130.10837562800092435No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9880.10570100736911478No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA9810.10495211359220807No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA9780.10463115911639094No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC9650.10324035638784995No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9640.10313337156257756No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9600.10270543226148802No Hit
GCTTGGTCCCTCCGCCGAACAGCAACTTGTTGCTGTCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAG9430.1008866902318575No Hit
CATATGCAGACAGCAACAAGTTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC9430.1008866902318575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.00426636259.38970
GTACTAG200.0077652551.0506821
TGGTATC2050.046.4777152
GGTATCA6050.042.753191
GTGGTAT2400.041.1241571
AATGGTA3800.038.29027670
GTATCAA16250.037.9084051
AAAACCG1001.8148437E-434.64358570
CCTATAC400.002777359734.0283243
TATCAAC18050.033.9340632
TCTGGAA10550.032.1807770
TCAACGC19300.032.0888864
CAACGCA19500.031.5886465
ATCAACG19400.031.5726743
AACGCAG19900.031.1280426
CGCAGAG21450.028.732328
AGAGTAC21350.028.71817411
CAGAGTA21700.028.56892210
CGGGTCG605.841274E-428.38578610
GCAGAGT22200.027.7646089
Produced by FastQC (version 0.11.5)