FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628826
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAAGCTGTTGACAGTGATAAGTTGCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC33550.533533918762901No Hit
GCTTAATAGTTACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28280.4497269514937359No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27240.4331881951446028No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26610.4231695254331087No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21780.3463597243116538No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19310.30708017798246257No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17740.28211301695540575No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17670.28099983143190643No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17410.27686514234462317No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA16950.2695499231901989No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16930.26923187018348477No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16620.26430204857941625No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16500.2623937305391317No Hit
TCCTTACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGAC16450.2615985980223464No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16340.2598493064854189No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15940.25348824635113687No Hit
GTCTCCATCCTTACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGGGCGTTACCA15700.24967161027056767No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGGGCGTTACCACTTATTTAGCCTGGTTTCAGCAAAAA15490.24633205370006966No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGC15120.24044807307585883No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15080.23981196706243063No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14930.23742656951207486No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14090.22406834323008273No Hit
GTCAGGGCGTTACCACTTATTTAGCCTGGTTTCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTG13540.215321885545445No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13360.21245940848501813No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13320.21182330247158992No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13050.20752958688094958No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT12700.20196365926345283No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12590.20021436772652532No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12570.1998963147198112No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11540.1835165848740351No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAAT11500.18288047886060693No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG10860.17270278264575575No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10780.17143057061889935No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTA10630.1690451730685436No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTCTGCAACTTATCACTGTCAACAGCTTAATAGTTAC10560.16793198754504424No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10500.16697782852490195No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10450.1661826960081167No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10370.16491048398126032No Hit
GGTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAATAAGTGGTA10250.16300216594097572No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10000.1590265033570495No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9650.15346057573955274No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG9640.15330154923619568No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9490.15091615168583997No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9470.15059809867912585No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9230.14678146259855668No Hit
GTGATAAGTTGCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG9170.1458273035784144No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9110.14487314455827208No Hit
CAGTAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG9100.14471411805491505No Hit
ACTTATCACTGTCAACAGCTTAATAGTTACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA9000.14312385302134453No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8870.14105650847770287No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA8480.13485447484677796No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTG8410.1337412893232786No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8340.13262810379977927No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8330.13246907729642224No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8200.13040173275278058No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8140.1294475737326383No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8130.12928854722928124No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT8090.12865244121585304No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7980.1269031496789255No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7970.12674412317556843No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7830.12451775212856975No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7780.12372261961178449No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7770.12356359310842745No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7710.12260943408828515No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT7640.1214962485647858No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7620.1211781955580717No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT7610.12101916905471466No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7610.12101916905471466No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT7490.11911085101443007No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC7400.11767961248421663No Hit
CGCCCTGACTGGTCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA7370.11720253297414548No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7360.11704350647078843No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7310.11624837395400318No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGG7200.11449908241707563No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7140.11354492339693333No Hit
GTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAATAAGTGGTAACG7130.11338589689357627No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7010.11147757885329168No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6920.11004634032307824No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6830.1086151017928648No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6700.10654775724922316No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6690.1063887307458661No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT6670.106070677739152No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC6660.10591165123579496No Hit
GTCTCCTACAGATGCAGACAGTAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG6440.10241306816193987No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6410.10193598865186873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1750.054.2921521
ATTAGTG200.00785600150.89893
TACGAGG200.00785600150.89893
GCCGTAG507.9058736E-847.5056341
ACGAGAC1950.047.0135122
GACCGGA2000.045.8746826
TAGCTCG306.512364E-445.70509336
AGTTCGG306.5874786E-445.59885834
ATCTCGT1750.045.12830437
TATCTCG1750.045.05216236
AGACCGG2050.044.7522225
GTATGCC2100.044.46573342
TCGTATG2100.044.36863340
ACCGGAG2150.044.26874527
CCGTCTT2050.044.19999347
TGGTATC2150.044.191292
GGTATCA6800.043.9127731
GAGCCTA2100.043.822831
CTTATAC2100.043.627631
ATGCCGT2150.043.54105844
Produced by FastQC (version 0.11.5)