FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628826
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAAGCTGTTGACAGTGATAAGTTGCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC31810.5058633071787744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27120.4312798771043182No Hit
GCTTAATAGTTACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24310.3865934296609873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21640.3441333532646551No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21380.3399986641773718No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19100.3037406214119645No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18960.3015142503649658No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16560.26334788955927396No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16330.2596902799820618No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16220.2579409884451343No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15800.2512618753041382No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15770.25078479579406704No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA15320.2436286031429998No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14630.2326557744113634No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14600.23217869490129225No Hit
TCCTTACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGAC14590.2320196683979352No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14280.22708984679386665No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14230.22629471427708142No Hit
GTCTCCATCCTTACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGGGCGTTACCA14110.22438639623679682No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGC13660.2172302035857296No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGGGCGTTACCACTTATTTAGCCTGGTTTCAGCAAAAA12870.2046671098205227No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12790.2033948977936663No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12500.19878312919631183No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12180.19369428108888626No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12070.1919449895519587No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11920.189559592001603No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11710.18622003543110496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11690.18590198242439085No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11660.1854249029143197No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11240.17874578977332362No Hit
GTCAGGGCGTTACCACTTATTTAGCCTGGTTTCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTG11220.17842773676660953No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11200.17810968375989542No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAAT10530.1674549080349731No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT10520.16729588153161606No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG10440.16602366950475966No Hit
GGTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAATAAGTGGTA9770.15536889377983737No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTCTGCAACTTATCACTGTCAACAGCTTAATAGTTAC9690.15409668175298094No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTA9590.15250641671941045No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT9360.1488488071421983No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9170.1458273035784144No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9140.14535022406834325No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9050.1439189855381298No Hit
GTGATAAGTTGCAGAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG8900.14153358798777405No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8810.1401023494575606No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8720.13867111092734716No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8640.13739889890049076No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8630.1372398723971337No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8600.13676279288706256No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8360.13294615680649338No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8280.13167394477963698No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8240.13103783876620878No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8210.13056075925613764No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8070.12833438820913895No Hit
ACTTATCACTGTCAACAGCTTAATAGTTACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA7980.1269031496789255No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7800.12404067261849859No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTG7770.12356359310842745No Hit
CAGTAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG7690.12229138108157106No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7560.12022403653792942No Hit
GTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGAAACCAGGCTAAATAAGTGGTAACG7470.11879279800771597No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7320.11640740045736023No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7260.11545324143721794No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7210.11465810892043268No Hit
CGCCCTGACTGGTCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA7170.11402200290700447No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7130.11338589689357627No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7120.11322687039021923No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7100.11290881738350513No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6990.11115952584657758No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6840.10877412829622185No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA6840.10877412829622185No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6780.10781996927607955No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6770.1076609427727225No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGG6650.1057526247324379No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT6640.10559359822908086No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT6610.10511651871900972No Hit
GTCTCCTACAGATGCAGACAGTAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG6580.10463943920893856No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6480.10304917417536807No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT6410.10193598865186873No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG6330.10066377662501232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)