FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147478
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAGACCGGACCATTTAGC78490.6840218287409432No Hit
GTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC72350.6305131775946903No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG60080.5235830229424878No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56960.49639295916784465No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55300.4819264508774896No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51110.4454115895903886No Hit
GCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAGACCGG50450.439659845330368No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTC50200.4374811543227844No Hit
GTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCT49180.42859209501184337No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46700.4069794802166142No Hit
GATTATTATTGTGCTACATGGGATGACAAGCTAAATGGTCCGGTCTTCGG41730.3636671029858525No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38700.33726136797393935No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC38610.3364770392112093No Hit
ATAATAATCTGCTTCATCGTCGGACCGGAGCCCACTGATGACCAGGGAGG37270.32479925541056126No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG37190.3241020742881345No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36510.3181760347475071No Hit
CCCCTGAGGGCCGCCGATCATCTCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGATACCAGTATA35530.30963556599777947No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34540.30100794960774846No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33580.2926417761386275No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG33350.2906373804116506No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT32830.28610570311587674No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA32230.28087684469767615No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCAC31890.27791382492736244No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30500.2658003029251977No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30080.26214010203245725No Hit
CCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGTCATCAGTGGGCTCCGGT29540.25743412945607674No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG29170.254209666764853No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28970.2524667139587861No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA28900.2518566804766627No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28690.25002658003029254No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28280.24645352677785545No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27050.2357343670205442No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26980.23512433353842077No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25780.22466661670201954No Hit
CCTTGGTCCCTCCGCCGAAGACCGGACCATTTAGCTTGTCATCCCATGTAGCACAATAATAATCTGCTTCATCGT25780.22466661670201954No Hit
GTGCTACATGGGATGACAAGCTAAATGGTCCGGTCTTCGGCGGAGGGACC24720.21542896682986515No Hit
ACATGGGATGACAAGCTAAATGGTCCGGTCTTCGGCGGAGGGACCAAGGTGACCGTCCTAAGTCAGCCCAAGG24670.2149932286283484No Hit
ATGTAGCACAATAATAATCTGCTTCATCGTCGGACCGGAGCCCACTGATG24370.21237879941924812No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23890.20819571268468764No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG23620.2058427263964974No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23380.2037511830292171No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22970.20017812977678No Hit
GGTCATCAGTGGGCTCCGGTCCGACGATGAAGCAGATTATTATTGTGCTA22830.1989580628125332No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAGACCGGAC22690.19773799584828644No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22110.1926834327106925No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22090.1925091374300858No Hit
GTCAGGGACCCCTGAGGGCCGCCGATCATCTCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGATA21870.19059188934341223No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC21570.18797746013431194No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20600.17952413902488765No Hit
TCTTTAGAGATGATCGGCGGCCCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC20420.17795548149942744No Hit
GTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGT19960.17394669004547364No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19880.17324950892304689No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19880.17324950892304689No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19820.17272662308122683No Hit
CTGATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAGTCGGTC19800.17255232780062016No Hit
TCTCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGATACCA19720.17185514667819338No Hit
GGACCAAGGTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19520.17011219387212653No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19430.16932786510939643No Hit
GTCTGGCACCTCAGCCTCCCTGGTCATCAGTGGGCTCCGGTCCGACGATG18950.16514477837483596No Hit
GCTGATACCAGTATACATAATTTTTTCCGATGTCGGAGTTGCTTCCAGAA18930.16497048309422924No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTC18710.1630532350075557No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18670.16270464444634233No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18420.16052595343875872No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG18210.15869585299238853No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18110.15782437658935508No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17950.1564300143445016No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17750.15468706153843473No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17740.15459991389813138No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17570.15311840401297452No Hit
GCTAAATGGTCCGGTCTTCGGCGGAGGGACCAAGGTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA17400.1516368941278177No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17350.15120115592630098No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT17340.15111400828599764No Hit
ATCCCATGTAGCACAATAATAATCTGCTTCATCGTCGGACCGGAGCCCAC17320.15093971300539094No Hit
GTTTGGGGGCCGTTCCTGGGAACTGCTGATACCAGTATACATAATTTTTT17270.15050397480387423No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17080.1488481696381107No Hit
GGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGAGATGATCGGCGGCCCTCAGGGGTCC17050.1485867267172007No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG16970.14788954559477394No Hit
TCAGTGGGCTCCGGTCCGACGATGAAGCAGATTATTATTGTGCTACATGG16930.14754095503356057No Hit
CCATTTAGCTTGTCATCCCATGTAGCACAATAATAATCTGCTTCATCGTC16910.14736665975295388No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGA16820.14658233099022377No Hit
CTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGTCATCAGTGGGCTCC16740.14588514986779705No Hit
TTATTATTGTGCTACATGGGATGACAAGCTAAATGGTCCGGTCTTCGGCG16720.14571085458719032No Hit
CTCTTGTTCTGGAAGCAACTCCGACATCGGAAAAAATTATGTATACTGGTATCAGCAGTTCCCAGGAACGGCCCC16660.14518796874537027No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16420.14309642537809003No Hit
GTGCCAGACTTGGAGCCAGAGAGTCGGTCAGGGACCCCTGAGGGCCGCCG15880.1383904528017095No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15570.13568887595230586No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15560.1356017283120025No Hit
GTCTTCGGCGGAGGGACCAAGGTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC15550.13551458067169914No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT15300.13333588966411555No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14970.13046001753410524No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14780.12880421236834172No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14520.1265383737204548No Hit
CTGTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC14480.1261897831592414No Hit
AGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG14320.1247954209143879No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG14300.12462112563378122No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGT14130.12313961574862436No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAG14110.1229653204680177No Hit
GCCCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAG14040.12235528698589429No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14030.12226813934559093No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT13980.12183240114407423No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG13730.11965371013649063No Hit
GCCCCCAAACTCCTCATCTTTAGAGATGATCGGCGGCCCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGT13620.11869508609315385No Hit
GGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC13480.11747501912890705No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC13350.11634209980496359No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT13300.11590636160344686No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC13260.1155577710422335No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13240.1153834757616268No Hit
CAATAATAATCTGCTTCATCGTCGGACCGGAGCCCACTGATGACCAGGGA13200.11503488520041344No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG13140.11451199935859335No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAACTCCGACATCGGAAAAAATTATGTATA13040.11364052295555994No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAGAC12850.11198471778979642No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT12610.10989317442251616No Hit
GTTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGAGATGATCGGCGGCCCTCAGGGGTCCCTGACCGACTCT12580.10963173150160613No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT12550.1093702885806961No Hit
CCCTGGTCATCAGTGGGCTCCGGTCCGACGATGAAGCAGATTATTATTGT12500.10893455037917939No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC12470.10867310745826936No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12470.10867310745826936No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12450.10849881217766266No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12350.10762733577462923No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12210.10640726881038243No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12060.10510005420583228No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT12050.10501290656552893No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12020.1047514636446189No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC11990.10449002072370887No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC11970.10431572544310216No Hit
GAGCCAGAGAGTCGGTCAGGGACCCCTGAGGGCCGCCGATCATCTCTAAAGATGAGGAGTTTGGGGGCCGTTC11860.1033571013997654No Hit
CCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT11630.10135270567278849No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11600.10109126275187846No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11590.10100411511157512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)