FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147478
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC86190.7511255117745177No Hit
GTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCT57680.5026675892696854No Hit
GACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAGACCGGACCATTTAGC54790.47748192122201905No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54700.476697592459289No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53160.4632768558525741No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG52580.45822229271498016No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51440.44828746172039896No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC48320.42109739794575585No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45850.39957193079083No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTC40690.35460374839430475No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA39240.34196734055031996No Hit
GCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAGACCGG39050.3403115353845564No Hit
GATTATTATTGTGCTACATGGGATGACAAGCTAAATGGTCCGGTCTTCGG36710.319918987553574No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG36350.31678167250265366No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36170.3152130149771935No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCAC35630.3105070424008129No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG34450.3002236208450184No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34120.297347748715008No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34090.297086305794098No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33970.2960405341104579No Hit
CCCCTGAGGGCCGCCGATCATCTCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGATACCAGTATA33420.291247413893774No Hit
CCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGTCATCAGTGGGCTCCGGT31700.2762580197615989No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30830.268676175055208No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29920.2607457397876038No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29050.2531638950812129No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28810.25107235171393266No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28580.24906795598695575No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG28020.2441876881299685No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27460.23930742027298127No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27190.23695443398479102No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27020.23547292409963416No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG26450.23050550860234356No Hit
GTGCTACATGGGATGACAAGCTAAATGGTCCGGTCTTCGGCGGAGGGACC25170.21935061064351558No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23730.20680135043983414No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23650.2061041693174074No Hit
GGTCATCAGTGGGCTCCGGTCCGACGATGAAGCAGATTATTATTGTGCTA23640.20601702167710403No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC23300.20305400190679038No Hit
GTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGT22950.20000383449617337No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22860.19921950573344327No Hit
TCTTTAGAGATGATCGGCGGCCCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC22710.19791229112889308No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22550.1965179288840396No Hit
ACATGGGATGACAAGCTAAATGGTCCGGTCTTCGGCGGAGGGACCAAGGTGACCGTCCTAAGTCAGCCCAAGG22510.19616933832282626No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22470.19582074776161285No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21980.19155051338674903No Hit
CTCTTGTTCTGGAAGCAACTCCGACATCGGAAAAAATTATGTATACTGGTATCAGCAGTTCCCAGGAACGGCCCC21430.18675739317006512No Hit
GTCTGGCACCTCAGCCTCCCTGGTCATCAGTGGGCTCCGGTCCGACGATG21320.18579876912672835No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG21230.18501444036399828No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21130.18414296396096483No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20670.18013417250701103No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20080.17499246172911376No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19780.17237803252001344No Hit
CTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGTCATCAGTGGGCTCC19060.16610340241817273No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19010.16566766421665602No Hit
TCTCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGATACCA18920.16488333545392592No Hit
GGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGAGATGATCGGCGGCCCTCAGGGGTCC18920.16488333545392592No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18920.16488333545392592No Hit
GGACCAAGGTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG18750.1634018255687691No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG18650.16253034916573564No Hit
GTCAGGGACCCCTGAGGGCCGCCGATCATCTCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGATA18630.16235605388512897No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT18600.16209461096421893No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18580.16192031568361223No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18560.16174602040300554No Hit
GTTTGGGGGCCGTTCCTGGGAACTGCTGATACCAGTATACATAATTTTTT18490.16113598692088213No Hit
GCTGATACCAGTATACATAATTTTTTCCGATGTCGGAGTTGCTTCCAGAA18410.1604388057984554No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAGACCGGAC18270.1592187388342086No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18070.15747578602814172No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17940.15634286670419825No Hit
CTGATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAGTCGGTC17830.15538424266086148No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17730.15451276625782803No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGA17700.154251323336918No Hit
CCTTGGTCCCTCCGCCGAAGACCGGACCATTTAGCTTGTCATCCCATGTAGCACAATAATAATCTGCTTCATCGT17410.15172404176812104No Hit
GCCCCCAAACTCCTCATCTTTAGAGATGATCGGCGGCCCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGT17150.1494582031202341No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC17110.14910961255902075No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16650.14510082110506692No Hit
GCTAAATGGTCCGGTCTTCGGCGGAGGGACCAAGGTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA16580.14449078762294354No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16570.1444036399826402No Hit
GGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC16460.1434450159393034No Hit
TCAGTGGGCTCCGGTCCGACGATGAAGCAGATTATTATTGTGCTACATGG16430.14318357301839338No Hit
CTGTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16320.1422249489750566No Hit
GTGCCAGACTTGGAGCCAGAGAGTCGGTCAGGGACCCCTGAGGGCCGCCG15720.136996090556856No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15520.13525313775078912No Hit
GCCCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAG15500.13507884247018243No Hit
AGCCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG15450.13464310426866571No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15410.13429451370745235No Hit
TTATTATTGTGCTACATGGGATGACAAGCTAAATGGTCCGGTCTTCGGCG15300.13333588966411555No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC15180.13229011798047544No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTC15150.13202867505956542No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAACTCCGACATCGGAAAAAATTAT15070.13133149393713867No Hit
GTCTTCGGCGGAGGGACCAAGGTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC15060.13124434629683532No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14960.1303728698938019No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG14810.12906565528925174No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGT14720.12828132652652163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14710.1281941788862183No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14590.1271484072025782No Hit
GTTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGAGATGATCGGCGGCCCTCAGGGGTCCCTGACCGACTCT14570.12697411192197147No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAACTCCGACATCGGAAAAAATTATGTATA14540.12671266900106146No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAG14430.1257540449577247No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14420.12566689731742134No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14300.12462112563378122No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14070.1226167299068043No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13780.12008944833800735No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT13490.11756216676921039No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAGAC13450.11721357620799702No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13410.11686498564678365No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13380.11660354272587362No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13380.11660354272587362No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC13310.11599350924375021No Hit
ATAATAATCTGCTTCATCGTCGGACCGGAGCCCACTGATGACCAGGGAGG13210.11512203284071677No Hit
CCCTGGTCATCAGTGGGCTCCGGTCCGACGATGAAGCAGATTATTATTGT13170.11477344227950338No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12700.11067750318524625No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12600.10980602678221282No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC12430.10832451689705598No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT12400.10806307397614595No Hit
CTTTAGAGATGATCGGCGGCCCTCAGGGGTCCCTGACCGACTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC12180.1061458258894724No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12010.10466431600431554No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12000.1045771683640122No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11970.10431572544310216No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT11910.1037928396012821No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT11880.10353139668037208No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT11870.10344424904006876No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG11830.10309565847885536No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGC11750.10239847735642861No Hit
ATGTAGCACAATAATAATCTGCTTCATCGTCGGACCGGAGCCCACTGATG11740.10231132971612528No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAACTCCGACATCGGAAAA11740.10231132971612528No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC11560.10074267219066509No Hit
GAGCCAGAGAGTCGGTCAGGGACCCCTGAGGGCCGCCGATCATCTCTAAAGATGAGGAGTTTGGGGGCCGTTC11550.10065552455036175No Hit
GGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGAGAT11530.10048122926975506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCG503.0640658E-453.25306370
GTGGTAT2600.047.2756161
GTATCAA23150.038.6418841
TAACCGT451.0628187E-437.94887
AATAATA24350.036.90535470
TATCAAC25050.035.427552
ATCAACG26750.033.4312823
TCAACGC26950.033.056534
CAACGCA27200.032.8839235
AACGCAG27200.032.7655536
GCCGTTA553.4438874E-431.06662615
ACGCAGA29000.030.85637
AGAGTAC29100.030.65026911
CGCAGAG29150.030.5830218
CAGAGTA29350.030.268810
GCAGAGT29950.029.6559439
TACGTAC1501.8189894E-1229.61814512
CCGTTAA605.7340635E-428.476516
GCAATTA605.741385E-428.4702917
GTTAAAC605.7487143E-428.46408318
Produced by FastQC (version 0.11.5)