FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483761
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT55750.3757343669229748No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48870.3293657132112247No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC47290.3187170979692821No Hit
CCTATGAGCTGACTCAACCACCCTCAGTGTCCGTGTCCCCAGGACAGACA45990.30995557909932936No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA45890.30928161610933297No Hit
CCCCTGTCCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT44660.30099187133237765No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC41730.2812447557254841No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG40710.27437033322752113No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC39090.26345213278957996No Hit
GGACAGGGGACTGGCCTGGCTTCTGTTGATACCAGCAAGCATATTTATCC39040.2631151512945818No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC36750.24768139882366502No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC36170.24377241348168607No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34880.23507829091073293No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33350.22476665716378852No Hit
GACTATTACTGTCAGGCGTGGGACACCAGCACGGGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAG32510.21910536804781905No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCC32480.2189031791508201No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31110.20966988618786989No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG30130.20306504888590549No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCAGCACGGGAGTCTTCGGAACTGGGACCAAGGT30070.20266067109190763No Hit
CTATTACTGTCAGGCGTGGGACACCAGCACGGGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAGCC29720.20030180062692035No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29440.19841470425493055No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29020.1955840596969458No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28750.1937643596239556No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28460.19180986695296615No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG28160.18978797798297706No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC27840.18763129641498866No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG26590.17920675904003405No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGGGATAAATTGGGGGAT26170.17637611448204932No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTC25730.17341067732606533No Hit
GTCCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG25640.1728041106350686No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24880.1676819919110962No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23970.16154892870212925No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT23440.15797692485514853No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23130.15588763958615975No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG23050.15534846919416267No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT23020.15514628029716376No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22980.1548766951011652No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC22900.15433752470916812No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCTTGG22860.15406793951316958No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGA22660.15272001353317685No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22390.15090031346018665No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCAGCA21720.146384761427211No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCGTGCTGGTGTCCCACGCCTGACAGTAATAGTCAGC21400.14422807985922262No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA21210.14294755017822952No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCGTGCTGGTGTCCCACGCCTGACAGTAATAGTCAG20910.1409256612082404No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA20880.1407234723112415No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20510.13822980924825495No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA20420.13762324255725822No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20030.1349947868962724No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19980.1346578054012742No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG19370.13054663116229634No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19290.13000746077029926No Hit
GACCAGGACAGGGGACTGGCCTGGCTTCTGTTGATACCAGCAAGCATATT18960.12778338290331126No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18720.12616587172731997No Hit
GGCCAGTCCCCTGTCCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGG18510.1247505494483276No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG17960.12104375300334759No Hit
AGCCAGGCCAGTCCCCTGTCCTGGTCATCTATCAAGATACCAAGCGGCCC17560.11834790104336211No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17440.11753914545536646No Hit
GGATCCTTGGCCTCCTATGAGCTGACTCAACCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC17270.11639340837237265No Hit
GGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCAGCACGGGAGTCTTCGGAACTGGGACCAAGGTCACCGT17130.11544986018637772No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17110.11531506758837846No Hit
CACCTGCTCTGGGGATAAATTGGGGGATAAATATGCTTGCTGGTATCAACAGAAGCCAGGCCAGTCCCCTGTC16900.11389974530938607No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16780.11309098972139044No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16650.11221483783439515No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16550.1115408748443988No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16420.11066472295740352No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16290.10978857107040825No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16200.10918200437941152No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16180.10904721178141223No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16030.10803626729641769No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16000.10783407839941878No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15910.10722751170842205No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15870.1069579265124235No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15860.10689053021342386No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCTTGGCCTCCTATGAGCTGACT15820.10662094501742532No Hit
ACATGGAACTGAGCAGTCTGAGAACTGAGGACACGGCCGTGTATTTCTGT15500.10446426344943693No Hit
GTTCTCAGACTGCTCAGTTCCATGTAGGCTGTGCTTACGGAGGTGTTCCTGGTCATGGAGACTCTGCCCTGGAA15470.10426207455243802No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15400.10379030045944057No Hit
GTTCCGAAGACTCCCGTGCTGGTGTCCCACGCCTGACAGTAATAGTCAGC15370.10358811156244165No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15280.10298154487144494No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCTTGGCCTCCTATGAG15230.10264456337644676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG100.008353639137.2620470
GCGTATG301.11871486E-769.11613540
GTCGTAT252.560384E-455.2306739
CGCGTAT252.560384E-455.2306739
TGTCGTA252.5802906E-455.1441538
ATCGCGT252.5985736E-455.06538837
CGTATGC1500.050.730241
CATGTCG306.427065E-445.83171536
TCGTATG1250.044.23432540
GCCGTCT1700.040.92627346
GTGGTAT3350.040.7210661
ACGAGAC2100.040.60534722
ATGCCGT1650.039.979844
TATACAC2400.039.7878723
AGGGGGG2100.039.21772870
CACGAGA2250.037.89576721
ATCTCCG2300.037.07194510
CTTATAC2550.036.1100041
ATACACA2550.036.1100044
CCGTCTT1950.035.718647
Produced by FastQC (version 0.11.5)