FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483761
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49470.33340949115120294No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC48660.3279503909322324No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43270.29162378577142817No Hit
CCCCTGTCCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT41510.2797620371474921No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40800.27497689991851787No Hit
CCTATGAGCTGACTCAACCACCCTCAGTGTCCGTGTCCCCAGGACAGACA38980.262710773500584No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38380.2586669955606058No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC37070.24983808039165337No Hit
GGACAGGGGACTGGCCTGGCTTCTGTTGATACCAGCAAGCATATTTATCC36620.24680524693666972No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36560.2464008691426719No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36030.24282886529569114No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34170.2302931536817587No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32060.21607253459283537No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31700.21364626782884844No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG30880.20811977131087825No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30760.20731101572288257No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCC30540.2058282971448906No Hit
GACTATTACTGTCAGGCGTGGGACACCAGCACGGGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAG29420.19827991165693126No Hit
CTATTACTGTCAGGCGTGGGACACCAGCACGGGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAGCC28490.19201205584996506No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28270.19052933727197305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27850.1876986927139883No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27090.18257657399001592No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCAGCACGGGAGTCTTCGGAACTGGGACCAAGGT27090.18257657399001592No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26250.17691528487404642No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG25990.17516298110005588No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTC25020.1686255400970911No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC24790.16707542522009947No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24710.1665362548281024No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23810.16047058791813507No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG23650.15939224713414088No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22720.15312439132717467No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGGGATAAATTGGGGGAT22690.15292220243017574No Hit
GTCCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG22690.15292220243017574No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22650.1526526172341772No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT22600.152315635739179No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGA21860.1473283096132059No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21580.14544121324121606No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA21220.14301494647722915No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21180.1427453612812306No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21050.14186920939423533No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT20720.13964513152724733No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20610.13890377223825132No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCTTGG20310.1368818832682622No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19990.13472520170027383No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19940.13438822020527566No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19820.13357946461728No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCGTGCTGGTGTCCCACGCCTGACAGTAATAGTCAGC19310.13014225336829854No Hit
GACCAGGACAGGGGACTGGCCTGGCTTCTGTTGATACCAGCAAGCATATT18990.12798557180031014No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18860.12710941991331487No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA18560.12508753094332578No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCAGCA18480.12454836055132867No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCGTGCTGGTGTCCCACGCCTGACAGTAATAGTCAG18200.12266126417933886No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17630.11881967513635956No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG17300.11659559726937155No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17150.115584652784377No Hit
GGCCAGTCCCCTGTCCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGG16970.11437151940238355No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16870.11369755641238717No Hit
GGATCCTTGGCCTCCTATGAGCTGACTCAACCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC16810.11329317861838936No Hit
CACCTGCTCTGGGGATAAATTGGGGGATAAATATGCTTGCTGGTATCAACAGAAGCCAGGCCAGTCCCCTGTC16740.11282140452539188No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16690.1124844230303937No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16250.1095189858744097No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15800.10648615241942604No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15780.10635135982142677No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG15720.10594698202742894No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG15710.1058795857284293No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA15520.10459905604743622No Hit
AGCCAGGCCAGTCCCCTGTCCTGGTCATCTATCAAGATACCAAGCGGCCC15420.10392509305743984No Hit
GGATGAGGCTGACTATTACTGTCAGGCGTGGGACACCAGCACGGGAGTCTTCGGAACTGGGACCAAGGTCACCGT15410.1038576967584402No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15410.1038576967584402No Hit
GTATCTTGATAGATGACCAGGACAGGGGACTGGCCTGGCTTCTGTTGATA15350.10345331896444239No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15250.10277935597444601No Hit
GTTCTCAGACTGCTCAGTTCCATGTAGGCTGTGCTTACGGAGGTGTTCCTGGTCATGGAGACTCTGCCCTGGAA15110.10183580778845111No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15010.10116184479845473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)