FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827235
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27840.33654282035939004No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25390.3069260850906937No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21470.25953930866077957No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17650.21336137856836326No Hit
GGTATAGTCTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC17480.211306339794617No Hit
CTCCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAGACTATACCTATGCCTCCACCTTCGG17150.20731714688087421No Hit
GACTATACCTATGCCTCCACCTTCGGCCCTGGGACCAGAGTGGATGTCAAACGAACTGTGGCTGCACCAT17090.20659183907837556No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16690.20175645372838433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15720.19003064425465557No Hit
GTCTCCATCCTCCCTGTCAGCTTCTATAGGAGACAGAGTCACCATAACTT15470.18700852841091103No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15320.18519525890466432No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15190.18362375866591718No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15190.18362375866591718No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14450.17467829576843338No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13460.16271071702720508No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13280.16053479361970904No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12950.15654560070596626No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12870.15557852363596802No Hit
GATTTCACTCTCTCCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAGACTATACCTATGC12800.15473233119971955No Hit
AGTCACGGCATTGCCAATTATTTAGCCTGGTATCAGCAAAAACCAGGCAG12120.14651217610473444No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11760.14216032928974234No Hit
GTGGAGGCACCATAGATCAGGAGTTTAGGAGCTCTGCCTGGTTTTTGCTGATACCAGGCTAAATAATTGGCAATG11610.14034705978349563No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCT11600.14022617514974584No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11370.13744582857350088No Hit
CTATAGGAGACAGAGTCACCATAACTTGCCGGACGAGTCACGGCATTGCC11300.1365996361372524No Hit
GATCTATGGTGCCTCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11210.1355116744335044No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10980.13273132785725944No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT10860.13128071225226207No Hit
ACTTATTACTGTCAAGACTATACCTATGCCTCCACCTTCGGCCCTGGGAC10710.12946744274601535No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10650.12874213494351666No Hit
ATAGGAGACAGAGTCACCATAACTTGCCGGACGAGTCACGGCATTGCCAATTATTTAGCCTGGTATCAGCAAAAA10630.12850036567601708No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10490.12680798080352015No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10430.12608267300102147No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG10280.12426940349477475No Hit
CCATAGATCAGGAGTTTAGGAGCTCTGCCTGGTTTTTGCTGATACCAGGC10090.12197259545352893No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTCCCAGATCCACTGCCA10000.1208846337497809No Hit
GAGTGGATGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9900.11967578741228309No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9750.11786251790603637No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9700.11725809473728747No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGAAGCAG9630.116411902301039No Hit
CACCATAACTTGCCGGACGAGTCACGGCATTGCCAATTATTTAGCCTGGTATCAGCAAAAACCAGGCAGAGCTC9600.11604924839978967No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9540.11532394059729097No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGAAGCAGCTGTGAGAA9520.11508217132979143No Hit
GCTGATGGAGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAG9450.11423597889354295No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9240.11169740158479755No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8950.1081917472060539No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGAAGCAGCTGTGAGAACCAGATGACCCAGTCTCCATCCTCC8890.10746643940355521No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8880.10734555476980542No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8530.10311459258856309No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8520.10299370795481332No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8490.10263105405356399No Hit
AGACTATACCTATGCCTCCACCTTCGGCCCTGGGACCAGAGTGGATGTCAAACGAACTGTGGCTGCACCAT8300.10033424601231815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG250.001344570479.4648770
GACGTTA200.002904272265.4464769
TCGTATG700.059.1893340
CGTATGC800.056.1721741
GATCCGG200.0068826152.6319550
TAAGCGC200.00701103752.38648247
GCCGTCT750.051.1364846
TAAGCCG200.0078381550.9296310
ATACCGC200.00784189750.923477
CCGTCTT901.8189894E-1242.6852847
ACGAGAC1150.041.35169222
CCCACGA1150.041.33669319
GTATCAA24550.041.2089461
TTAACGG504.0339382E-641.1065335
GTGGTAT2350.040.4498521
TATCAAC26950.037.035252
GAGCGAT1200.037.0174932
TCTCCGA1300.036.5648611
CGAGACA1400.036.40691823
ATCTCGT853.088644E-936.37919637
Produced by FastQC (version 0.11.5)