FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827235
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27210.3289270884331538No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20260.24491226797705606No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17940.21686703294710696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17370.2099766088233694No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16620.20091026129213588No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16400.19825079934964068No Hit
GGTATAGTCTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC16330.19740460691339223No Hit
GACTATACCTATGCCTCCACCTTCGGCCCTGGGACCAGAGTGGATGTCAAACGAACTGTGGCTGCACCAT15800.1909977213246538No Hit
CTCCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAGACTATACCTATGCCTCCACCTTCGG15770.19063506742340447No Hit
GTCTCCATCCTCCCTGTCAGCTTCTATAGGAGACAGAGTCACCATAACTT14350.17346944943093556No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14270.17250237236093735No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13430.16234806312595576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12940.1564247160722165No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12810.15485321583346934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12290.1485672148784807No Hit
GATTTCACTCTCTCCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAGACTATACCTATGC12100.14627040683723488No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11800.14264386782474145No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCT11420.1380502517422498No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11410.1379293671085No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11390.13768759784100043No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11290.13647875150350264No Hit
GTGGAGGCACCATAGATCAGGAGTTTAGGAGCTCTGCCTGGTTTTTGCTGATACCAGGCTAAATAATTGGCAATG11230.13575344370100395No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11080.13394017419475723No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10970.13261044322350965No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10860.13128071225226207No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10800.13055540444976338No Hit
AGTCACGGCATTGCCAATTATTTAGCCTGGTATCAGCAAAAACCAGGCAG10790.1304345198160136No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10590.12801682714101797No Hit
GATCTATGGTGCCTCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA10300.12451117276227432No Hit
CTATAGGAGACAGAGTCACCATAACTTGCCGGACGAGTCACGGCATTGCC10270.12414851886102499No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG10170.12293967252352718No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT10010.12100551838353069No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9930.12003844131353242No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9900.11967578741228309No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTCCCAGATCCACTGCCA9740.1177416332722866No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG9660.11677455620228835No Hit
GAGTGGATGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9390.11351067109104426No Hit
ACTTATTACTGTCAAGACTATACCTATGCCTCCACCTTCGGCCCTGGGAC9330.11278536328854558No Hit
ATAGGAGACAGAGTCACCATAACTTGCCGGACGAGTCACGGCATTGCCAATTATTTAGCCTGGTATCAGCAAAAA9190.11109297841604863No Hit
GCTGATGGAGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAG9140.11048855524729974No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGAAGCAG9010.10891705500855259No Hit
CACCATAACTTGCCGGACGAGTCACGGCATTGCCAATTATTTAGCCTGGTATCAGCAAAAACCAGGCAGAGCTC8890.10746643940355521No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8870.10722467013605566No Hit
CCATAGATCAGGAGTTTAGGAGCTCTGCCTGGTTTTTGCTGATACCAGGC8780.10613670843230762No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8750.10577405453105829No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGGAAGCAGCTGTGAGAA8580.103719015757312No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8330.10069689991356748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)