FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086806
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35990.3311538581862816No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31690.2915883791587459No Hit
CTAATATACTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC31240.28744780577214335No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29530.27171362690305356No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27370.25183887464736116No Hit
GAGTATAAGCAACTGGTTGGCCTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATGCCTC27200.25027465803464466No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27010.24852641593807911No Hit
GAAATACACTGCCATGTCTGCGACGATCACAGAAGTCAGCCTCAGGGAGA26600.2447538935191745No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25430.23398840271400784No Hit
GTATATTAGTTATTTCGTTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT25150.23141204594012177No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24710.22736348529544373No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24660.2269034215858212No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24320.22377498836038812No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24180.2224868099734451No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23910.22000246594148357No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23010.21172131916827844No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT20890.19221461788028407No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20760.19101845223526553No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20720.19065040126756752No Hit
CTTATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGA20710.19055838852564305No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20120.18512963675209743No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19450.17896478304315583No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19420.1786887448173823No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19170.17638842626926976No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18870.17362804401153473No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18680.17187980191496918No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18390.16921143239915865No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17700.162862553206368No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATGTTGCAACTTA17620.162126451270972No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17530.1612983365936515No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17510.16111431110980248No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17320.15936606901323694No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17280.15899801804553895No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17200.15826191611014293No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16950.15596159756203037No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16020.14740441256305173No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15990.14712837433727824No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15730.1447360430472412No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15160.1394913167575446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15030.13829515111252608No Hit
GAGTGAATTCTGTCCCGGATCCACTGCCGCTGAACCTTGATGGGACCCCA14960.13765106191905455No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14840.13654690901596053No Hit
CTATAGCAGTGGATGGTACCCTTTTGACTACTGGGGCCAGGGAACCCTTGTCACCGTCTCCTCAGCATCCCCGAC14780.13599483256441353No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14710.135350743370942No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14420.13268237385513146No Hit
CTGCTATAGTCCCCTCTCGCACAGAAATACACTGCCATGTCTGCGACGATCACAGAAGTCAGCCTCAGGGAGAA14360.13213029740358445No Hit
GTGTATTTCTGTGCGAGAGGGGACTATAGCAGTGGATGGTACCCTTTTGA14360.13213029740358445No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14290.13148620821011295No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14050.1292779024039249No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13870.12762167304928387No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13810.12706959659773684No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13770.12670154563003885No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13710.12614946917849185No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13690.12596544369464283No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATAAGCAA13580.12495330353347331No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13570.12486129079154881No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13550.1246772653076998No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13410.12338908692075677No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13260.12200889579188927No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCAGTAGTAATTACTACTGCTGATGGAG13210.12154883208226674No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13210.12154883208226674No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13190.12136480659841774No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13130.12081273014687074No Hit
ACCTTGATGGGACCCCACTTTCCAAGCTGGAGGCATCAGAGATCAGGAGCTTAGGGGCTTTCCCTGATTTCTG12910.1187884498245317No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12890.1186044243406827No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12860.1183283861149092No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12800.11777630966336218No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12730.11713222046989068No Hit
GCTATAGTCCCCTCTCGCACAGAAATACACTGCCATGTCTGCGACGATCA12710.11694819498604167No Hit
GTAATAAGTTGCAACATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCGGATCCACTGCCG12580.11575202934102315No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12510.11510794014755164No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12430.11437183821215562No Hit
GTCATGGATCTCATGTGCATGCAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC12230.11253158337366559No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12220.11243957063174108No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12180.11207151966404306No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12080.11115139224479806No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11970.11013925208362854No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCATGCAAATGAAGCACCTGTGGTT11950.10995522659977953No Hit
GCTTAGGGGCTTTCCCTGATTTCTGCTGATACCAGGCCAACCAGTTGCTT11860.10912711192245902No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11860.10912711192245902No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11840.10894308643861002No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC11750.10811497176128951No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11750.10811497176128951No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11750.10811497176128951No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11740.10802295901936501No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11720.107838933535516No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11470.10553861498740345No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11470.10553861498740345No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11370.10461848756815845No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11350.10443446208430944No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11110.1022261562781214No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCAATGTCTCTGGTGGCTCCATCAGCAGTAGTAATTA10930.10056992692348037No Hit
ACCCAGTATCCTTCCACCTTGTCTGCATCTGTCGGAGACAGAGTCACCAT10890.10020187595578237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATA100.008167382138.2979470
TACGTAT250.001132490282.9787770
GTACGTA250.007362929651.74196669
GTGGTAT2200.049.7302471
ATGCCGT2250.044.65408344
ACGAGAC2300.044.6114822
ACATGCG2150.044.58335527
CGTATGC2250.044.5626741
CGAGACA2400.044.1797923
GACAGGT1950.043.8327521
TACACAT2350.043.648245
CCCACGA2300.043.11847719
TATACAC2300.043.108563
GTATCAA22500.042.5469931
TCGTATG2200.042.39090740
GATCTCG1950.042.35811236
CATGCGC2200.042.03532428
ATCTCGT2000.041.3336237
TTATACA2500.039.6598742
CGAGCCC2500.039.65987415
Produced by FastQC (version 0.11.5)