FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086806
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29730.27355388174154355No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29620.27254174158037403No Hit
CTAATATACTGTTGGCAGTAATAAGTTGCAACATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC28420.26150021254943384No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28410.26140819980750934No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26120.24033728190679843No Hit
GTATATTAGTTATTTCGTTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT25360.2333443135205363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25020.2302158802951033No Hit
GAAATACACTGCCATGTCTGCGACGATCACAGAAGTCAGCCTCAGGGAGA24090.22165869529612459No Hit
GAGTATAAGCAACTGGTTGGCCTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGCTCCTGATCTCTGATGCCTC23850.21945038948993656No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23760.21862227481261606No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22940.2110772299748069No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22850.21024911529748638No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22830.2100650898136374No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21750.20012771368579121No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20910.19239864336413307No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20810.19147851594488804No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20770.19111046497719003No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20470.18835008271945497No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19580.18016094868817434No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18800.17298395481806322No Hit
GAGTGAATTCTGTCCCGGATCCACTGCCGCTGAACCTTGATGGGACCCCA18630.17141973820534667No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18100.16654306288334808No Hit
CTTATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGA17930.16497884627063156No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17730.16313859143214152No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17640.16231047675482102No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17200.15826191611014293No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17170.15798587788436944No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16860.15513348288470988No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16640.15310920256237084No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16630.15301718982044635No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATGTTGCAACTTA15840.1457481832084107No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15620.14372390288607167No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15560.14317182643452464No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15530.14289578820875115No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15450.14215968627335512No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15330.1410555333702611No Hit
CTGCTATAGTCCCCTCTCGCACAGAAATACACTGCCATGTCTGCGACGATCACAGAAGTCAGCCTCAGGGAGAA14710.135350743370942No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14570.134062564983999No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14270.13130218272626393No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14060.1293699151458494No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14040.1291858896620004No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13920.12808173675890636No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13850.12743764756543485No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13620.12532135450117132No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13480.1240331761142283No Hit
GTGTATTTCTGTGCGAGAGGGGACTATAGCAGTGGATGGTACCCTTTTGA13450.12375713788845479No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13390.12320506143690779No Hit
CTATAGCAGTGGATGGTACCCTTTTGACTACTGGGGCCAGGGAACCCTTGTCACCGTCTCCTCAGCATCCCCGAC13200.12145681934034225No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13100.12053669192109723No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12920.1188804625664562No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12890.1186044243406827No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12850.11823637337298469No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12800.11777630966336218No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12760.11740825869566418No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCATGCAAATGAAGCACCTGTGGTT12670.11658014401834366No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12570.11566001659909864No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12540.11538397837332513No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATAAGCAA12510.11510794014755164No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12370.11381976176060861No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12280.11299164708328809No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12090.11124340498672255No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCAGTAGTAATTACTACTGCTGATGGAG12050.11087535401902454No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12010.11050730305132656No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11920.10967918837400603No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11900.10949516289015702No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC11860.10912711192245902No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11820.10875906095476101No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11710.1077469207935915No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11680.10747088256781799No Hit
GGGCTGGAGTGGATTGGGAGCGTCTATTATAATGGGAATTCCCGCTACAA11680.10747088256781799No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11660.10728685708396898No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11630.1070108188581955No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11610.10682679337434649No Hit
GTAATAAGTTGCAACATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCGGATCCACTGCCG11570.10645874240664847No Hit
GCTATAGTCCCCTCTCGCACAGAAATACACTGCCATGTCTGCGACGATCA11530.10609069143895046No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11410.10498653853585643No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11310.10406641111661143No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11280.10379037289083791No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11170.10277823272966839No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11140.10250219450389492No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11090.10204213079427239No Hit
ACCTTGATGGGACCCCACTTTCCAAGCTGGAGGCATCAGAGATCAGGAGCTTAGGGGCTTTCCCTGATTTCTG10990.10112200337502739No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAT150.002381842668.806836
CCTAACG200.00764626751.2510382
CTAACGC200.007656015551.2345243
ATGCATG658.071788E-443.7546170
CACGGAT10400.041.7036170
GTATCAA21450.041.2727971
GTCGGCT350.001203776240.34513559
ACGGGTA853.263267E-936.17720416
GTGGTAT1350.035.4475061
TATCAAC25100.034.7118572
CTATTCT501.9773238E-434.146924
TCAACGC26050.033.6881264
ATCAACG26150.033.4379543
AACGCAG26500.033.2418636
CAACGCA26350.033.0438845
ACGCAGA27750.031.7444827
CATGCAT550.0040069531.57483369
GCTTGGG1307.2759576E-1231.5521761
CGCAGAG28350.031.1930778
AGAGTAC29300.030.52848611
Produced by FastQC (version 0.11.5)