FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946129
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAATCCGGGGTCCCT58960.6231708361122004No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56860.6009751312981634No Hit
ATTTTATACAGCTCCAACAATAGGGACTACTTAGCTTGGTATCAGCAGAA54400.5749744485160058No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49960.5280463869091847No Hit
GCTTAGGAGCCTGGCCTGGCTTCTGCTGATACCAAGCTAAGTAGTCCCTA49850.5268837547522589No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39270.4150596800224916No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38300.40480737827505553No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33890.35819639816557786No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33810.3573508475059955No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33170.3505864422293366No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33020.3490010347426197No Hit
CATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGAACTGTGGCTGCACCA29920.31623594668380317No Hit
GTTTATTACTGTCACCAATATTATCTTTCTCCGTACACATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGAA28970.30619503260126263No Hit
CTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATATTTTATACAGCTCCAA28950.30598364493636704No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28850.3049267066118891No Hit
GTATAAAATATTGTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGTCAGGGAGTCTGGAGA28720.30355268679006775No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27500.29065803923143674No Hit
GTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC26880.28410502161967344No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26790.2831537771276433No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26760.2828366956302999No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26610.28125128814358297No Hit
GTGCAGCCACAGTTCGCTTCATCTCCAACTTGGTCCCCTGGCCAAATGTG26570.28082851281379173No Hit
ATATTATCTTTCTCCGTACACATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGAACTGTGGCTGCACCAT26410.27913741149462706No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25730.271950230888177No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25020.26444596878438353No Hit
GGCCAAATGTGTACGGAGAAAGATAATATTGGTGACAGTAATAAACTGCC24910.2632833366274578No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24660.2606409908162629No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24450.2584214203348592No Hit
GTAATAAACTGCCAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA24120.25493352386408197No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23950.25313672871246945No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23740.25091715823106575No Hit
GTAATACACAGCCGTGTCCTCAGCTCGCAGGCTGTTCATTTGTAGAAACA22630.2391851428293605No Hit
GTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATATTTTATA22400.2367541846830612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTCATCTCCAACTTGGTCC21640.22872145341702876No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC20970.22163996664302646No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGG20520.2168837441828757No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATTTGGCAGTTTATTACTGTCACCAATATTATCTTTCT20190.21339584771209846No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19230.20324923979711013No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19170.20261507680242336No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19170.20261507680242336No Hit
TTCCCGGACAGATGCCCAGTAAATGAGCAGCTTAGGAGCCTGGCCTGGCTTCTGCTGATACCAAGCTAAGTAGTC19120.20208660764018438No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18740.19807024200716813No Hit
GCTCATTTACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT18690.1975417728449292No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18530.19585067152576444No Hit
GTCAGGGACCCCGGATTCCCGGACAGATGCCCAGTAAATGAGCAGCTTAGGAGCCTGGCCTGGCTTCTGCTGATA18340.19384248870925636No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18310.19352540721191297No Hit
ACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATT18310.19352540721191297No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATTTGGCAGTTTATTACTGTCACC17940.18961473541134452No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17750.18760655259483644No Hit
CCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17740.18750085876238864No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17370.18359018696182022No Hit
CAATAGGGACTACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCAT17290.18274463630223786No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17150.18126492264796873No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16620.17566314952823558No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16370.1730208037170407No Hit
GTAAATGAGCAGCTTAGGAGCCTGGCCTGGCTTCTGCTGATACCAAGCTA16310.17238664072235393No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15800.16699625526751638No Hit
GTGTATTACTGTGCGAAAAAAAAGTCTCTCTACTACTATTACATGGACGT15560.1644596032887693No Hit
AGATAATATTGGTGACAGTAATAAACTGCCAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15340.1621343389749178No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15220.16086601298554426No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15190.16054893148820087No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15180.16044323765575305No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14880.15727242268231922No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14710.1554756275307067No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14450.15272758788706403No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14360.15177634339503387No Hit
CAGTAATAAACTGCCAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14110.149133997583839No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATT14090.1489226099189434No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13990.14786567159446545No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13830.14617457027530073No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13830.14617457027530073No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13760.14543471344816616No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13630.14406069362634483No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13560.14332083679921026No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATATTTTATACAGCTCCAACAATAGGGACTA13460.1422638984747323No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13440.14205251080983672No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13410.14173542931249333No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13310.14067849098801538No Hit
GCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATT13280.140361409490672No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13100.13845892050661168No Hit
ATCTTTCTCCGTACACATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGA12780.13507671786828224No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12780.13507671786828224No Hit
GTACACATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGAACTGTGGCTG12760.13486533020338665No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12650.1337026980464609No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12550.13264575972198295No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12550.13264575972198295No Hit
TTATTACTGTCACCAATATTATCTTTCTCCGTACACATTTGGCCAGGGGA12420.13127173990016158No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12280.12979202624589248No Hit
GCTCCAACAATAGGGACTACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGG12260.1295806385809969No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12190.12884078175386232No Hit
CTACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAATC12140.12831231259162335No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12050.1273610680995932No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11940.12619843594266744No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGAC11930.12609274211021965No Hit
AGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAA11540.12197068264475563No Hit
AATGTGTACGGAGAAAGATAATATTGGTGACAGTAATAAACTGCCAAATC11500.12154790731496445No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11470.12123082581762107No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG11360.12006819366069531No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11340.11985680599579972No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11330.11975111216335194No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11270.11911694916866516No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11240.11879986767132178No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11220.11858848000642619No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11180.11816570467663501No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11060.11689737868726147No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGAC10840.11457211437340996No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10770.1138322575462754No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGACAGATGCCCAGTAAATGAGCAGCTTAGGAGCCTGGCCTGG10700.11309240071914084No Hit
GTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGA10590.11192976856221508No Hit
GGATCTCTGGTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGG10490.11087283023773714No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10450.11045005490794596No Hit
GGACTACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGC10440.11034436107549817No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGGCTACGGGGACATCGTGATGA10320.10907603508612462No Hit
GCCACAGTTCGCTTCATCTCCAACTTGGTCCCCTGGCCAAATGTGTACGG10190.10770201526430329No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9950.1051653632855562No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9900.10463689412331721No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9860.10421411879352603No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9820.10379134346373486No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9720.10273440513925691No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT9700.10252301747436132No Hit
CATTTACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA9600.10146607914988336No Hit
GCTAAGTAGTCCCTATTGTTGGAGCTGTATAAAATATTGTGGCTGGACTT9580.10125469148498778No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGCTTCATCTCCAACTTGGTCCCCTGGC9580.10125469148498778No Hit
CTGCAAGTCCAGCCACAATATTTTATACAGCTCCAACAATAGGGACTACTTAGCTTGGTATCAGCAGAAGCCAGG9540.1008319161551966No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9500.10040914082540542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)