FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946129
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56930.6017149881252979No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAATCCGGGGTCCCT55640.5880804837395324No Hit
ATTTTATACAGCTCCAACAATAGGGACTACTTAGCTTGGTATCAGCAGAA47830.5055336005978043No Hit
GCTTAGGAGCCTGGCCTGGCTTCTGCTGATACCAAGCTAAGTAGTCCCTA47130.49813503232645867No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40510.42816571524601826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39290.41527106768738725No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38850.4106205390596842No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34660.3663348232640581No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29950.31655302818114656No Hit
GTTTATTACTGTCACCAATATTATCTTTCTCCGTACACATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGAA28920.30566656343902365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28280.29890215816236476No Hit
CATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGAACTGTGGCTGCACCA28220.298267995167678No Hit
GTGCAGCCACAGTTCGCTTCATCTCCAACTTGGTCCCCTGGCCAAATGTG27600.2917149775559147No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27520.29086942689633233No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27300.28854416258248083No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27290.28843846875003304No Hit
GTAATAAACTGCCAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA27240.28790999958779406No Hit
CTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATATTTTATACAGCTCCAA26490.2799829621542094No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26430.27934879915952265No Hit
GGCCAAATGTGTACGGAGAAAGATAATATTGGTGACAGTAATAAACTGCC25860.27332425070999833No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25860.27332425070999833No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25760.2722673123855204No Hit
GTATAAAATATTGTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGTCAGGGAGTCTGGAGA25620.27078759873125124No Hit
ATATTATCTTTCTCCGTACACATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGAACTGTGGCTGCACCAT25510.2696249665743255No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25020.26444596878438353No Hit
GTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC24690.2609580723136063No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23810.2516570150582003No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23470.24806342475497525No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22100.23358336970962734No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21920.23168088072556703No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21910.23157518689311923No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGG21680.22914422874681992No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGAC21570.22798159658989417No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21340.2255506384435949No Hit
GTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATATTTTATA21160.2236481494595346No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20870.22058302831854854No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTCATCTCCAACTTGGTCC20330.2148755613663676No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19730.20853393141949988No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19690.2081111560897087No Hit
GTAATACACAGCCGTGTCCTCAGCTCGCAGGCTGTTCATTTGTAGAAACA19570.20684283010033513No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC19390.20494034111627485No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATTTGGCAGTTTATTACTGTCACCAATATTATCTTTCT19230.20324923979711013No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19030.2011353631481542No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18740.19807024200716813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18040.1906716737358225No Hit
GTCAGGGACCCCGGATTCCCGGACAGATGCCCAGTAAATGAGCAGCTTAGGAGCCTGGCCTGGCTTCTGCTGATA17780.18792363409217983No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17350.18337879929692463No Hit
TTCCCGGACAGATGCCCAGTAAATGAGCAGCTTAGGAGCCTGGCCTGGCTTCTGCTGATACCAAGCTAAGTAGTC17220.1820047794751033No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17050.18020798432349078No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16960.17925673983146062No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16600.17545176186334No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16520.17460621120375763No Hit
CCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16420.17354927287927965No Hit
AGATAATATTGGTGACAGTAATAAACTGCCAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC16340.17270372221969732No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATTTGGCAGTTTATTACTGTCACC16270.17196386539256275No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16160.170801233235637No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15940.16847596892178548No Hit
GCTCATTTACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT15850.16752472442975536No Hit
CAGTAATAAACTGCCAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15530.1641425217914259No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15480.16361405262918693No Hit
GTGTATTACTGTGCGAAAAAAAAGTCTCTCTACTACTATTACATGGACGT15270.16139448214778324No Hit
ACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATT15150.1601261561584097No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15130.1599147684935141No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14960.15811797334190159No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATT14760.15600409669294568No Hit
GTAAATGAGCAGCTTAGGAGCCTGGCCTGGCTTCTGCTGATACCAAGCTA14720.1555813213631545No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14430.1525162002221684No Hit
CAATAGGGACTACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCAT14360.15177634339503387No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14020.14818275309180884No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13810.14596318261040514No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13680.14458916278858377No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13540.14310944913431467No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13490.1425809799720757No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13480.1424752861396279No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13310.14067849098801538No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13280.140361409490672No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12960.13697920685234255No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12940.13676781918744696No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12440.1314831275650572No Hit
GTACACATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGAACTGTGGCTG12300.13000341391078807No Hit
ATCTTTCTCCGTACACATTTGGCCAGGGGACCAAGTTGGAGATGAAGCGA12270.12968633241344468No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12170.1286293940889667No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12000.1268325989373542No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11920.12598704827777182No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11890.12566996678042847No Hit
TTATTACTGTCACCAATATTATCTTTCTCCGTACACATTTGGCCAGGGGA11870.12545857911553288No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG11820.1249301099532939No Hit
AATGTGTACGGAGAAAGATAATATTGGTGACAGTAATAAACTGCCAAATC11780.12450733462350272No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11590.1224991518069946No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11590.1224991518069946No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATATTTTATACAGCTCCAACAATAGGGACTA11550.12207637647720343No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT11480.12133651965006886No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11370.1201738874931431No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11310.11953972449845635No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11270.11911694916866516No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11090.11721446018460484No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11080.11710876635215706No Hit
GCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATT11000.1162632156925747No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10990.1161575218601269No Hit
GCCACAGTTCGCTTCATCTCCAACTTGGTCCCCTGGCCAAATGTGTACGG10950.11573474653033572No Hit
GCTCCAACAATAGGGACTACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGG10910.11531197120054452No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10900.11520627736809673No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10900.11520627736809673No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10850.11467780820585775No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10770.1138322575462754No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGGCTACGGGGACATCGTGATGA10760.11372656371382761No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10430.11023866724305037No Hit
CTACTTAGCTTGGTATCAGCAGAAGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAATC10380.10971019808081138No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT10310.10897034125367683No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGAC10110.10685646460472091No Hit
GAGAAAGATAATATTGGTGACAGTAATAAACTGCCAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT10000.10569383244779518No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9930.10495397562066061No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG9810.10368564963128707No Hit
AGCCAGGCCAGGCTCCTAAGCTGCTCATTTACTGGGCATCTGTCCGGGAA9750.1030514866366003No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9640.10188885447967454No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTTCATGCCAACGTCAC9490.10030344699295762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCAA350.004725298457.87634370
CCCGATA200.00785894750.896072
GTGGTAT2200.047.8291551
CCTTTGT6450.042.92121570
GTATCAA13800.038.8625831
TATCAAC16050.033.40222
ATCAACG17000.031.93313
CAACGCA17300.031.7783095
TCAACGC17200.031.7590474
AACGCAG17550.031.5273256
CGCAGAG18200.030.4142138
GGGTATC450.00500508930.1717971
TAAAGGT450.005015450530.159044
CTATCCT450.005015450530.159044
TATTGTG21300.029.79861870
AGCGCTG3100.029.5572415
AGAGTAC18650.029.50451311
GCCCGAT604.917173E-429.23351151
TGCCCGA604.9645524E-429.18566750
CAGAGTA19000.029.136710
Produced by FastQC (version 0.11.5)