FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857245
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26060.30399710701141447No Hit
GTATTACTGCATGCAAACTCTACGAATCCCGCTCACTTTCGGCGGGGGGA25430.2966479827820518No Hit
CAGTAATACACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCAGTGCCA24180.2820663870888719No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19790.2308558230144241No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18540.21627422732124424No Hit
CTCCTATACTCTAATGGATACAATTACTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATG17450.20355907587679134No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17400.20297581204906412No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16140.18827756359033881No Hit
GTGTAAAATCTGTGCCTGATCCAGTGCCACTGAATCTGTCAGGGACCCCG15840.18477798062397566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15460.18034517553324894No Hit
GATTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTGTATTACTGCATGCAAACTCTACGAATC15140.1766122870357949No Hit
GGTCTAGTCAGAGCCTCCTATACTCTAATGGATACAATTACTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTC15010.1750958010837042No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14620.17054634322743206No Hit
GTAATACACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCAGTGCCA14620.17054634322743206No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA13210.15409830328552515No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGTAGATACA13090.15269847009897985No Hit
GTGTATTACTGCATGCAAACTCTACGAATCCCGCTCACTTTCGGCGGGGGGACCACGGTGGAGATCAAACGAA12930.15083202585025285No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12690.1480323594771623No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12510.1459326096973444No Hit
GTCTCCACCCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTATA12040.14044992971670875No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11810.13776691610916367No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11770.1373003050469819No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11200.13065109741089187No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11140.12995118081761925No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11000.12831804209998307No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCC10630.12400188977480184No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10560.12318532041598376No Hit
CCTATACTCTAATGGATACAATTACTTGGATTGGTACGTGCAGAAGCCAG10530.12283536211934744No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATGCATTTGGGTTCTAGTCGGGCC10370.12096891787062042No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10310.12026900127734777No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10030.11700272384207548No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT9900.11548623788998477No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9850.11490297406225758No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCCCCGCCGAAAGTG9850.11490297406225758No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9450.11023686344044002No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9300.10848707195725842No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9210.10743719706734947No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG9180.10708723877071316No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9110.10627066941189507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAC200.007635113351.2690413
GGTATCA10150.050.8985941
CCGTCAT554.733945E-448.75673770
CTTAGAC451.9812287E-645.583123
TCACTCG754.0467894E-544.69367670
TGGTATC2250.039.5122872
GTGGTAT2450.036.3079831
GTATCAA24900.035.4499171
CGTAATA400.002712145234.1933252
TCCGTCA550.00435048931.04768469
GACAGCC1251.382432E-1030.1076971
TATCAAC29250.029.8095632
AAAACCG900.005318477729.79578470
ATCAACG28950.029.7589243
TCAACGC29200.029.4989764
CAACGCA29700.028.887275
AACGCAG29950.028.6461416
GATTCAC1800.028.5110781
CCCGATA500.00687664928.272261
ACACCGG500.007031976228.14316458
Produced by FastQC (version 0.11.5)