FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695007
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18650.26834262100957257No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17330.24935000654669665No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15530.22345098682459313No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14940.2149618636934592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13670.19668866644508617No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12460.1792787698541166No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11590.16676091032176654No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG11080.15942285473383722No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10950.15755236997612973No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10940.15740848653322917No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10810.15553800177552168No Hit
CTATAAAACTTACCCATTCACTTTCGGCCCTGGGACCAAAGTTGATCTCAAAAGAACTGTGGCTGCACCAT10590.15237256603170904No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9980.14359567601477397No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9890.14230072502866878No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9450.13596985354104346No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9390.13510655288364004No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC9080.1306461661537222No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8500.12230092646548885No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8270.11899160727877561No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7980.11481898743465893No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC7820.11251685234824974No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA7270.1046032629887181No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7040.10129394380200486No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6980.10043064314460143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC750.055.38068441
TCGTATG700.054.20059640
GCGCTTA250.00911710949.0165468
GGTATCA15000.044.0268631
CTAAAGC359.1861485E-442.62143760
GTATGCC1100.037.9474942
GAACGCT350.001630142637.92967612
GTATCAA37800.037.751361
GTGGTAT4000.037.334341
ATGCCGT1250.036.42380544
ATCTCCG1151.8189894E-1234.62895610
ACCCTAA1203.6379788E-1233.34199527
TCGTTGC400.003139806433.1860771
TGGTATC4600.032.4646452
ATCTCGT1057.385097E-1032.42935637
CCCACGA1255.456968E-1231.870119
TACGTAT850.00400944231.5655170
TCTCCGA1309.094947E-1230.63330511
ATCAACG47700.030.1248873
TATACAC1451.8189894E-1229.7530353
Produced by FastQC (version 0.11.5)