FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695007
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16500.2374076807859489No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16160.23251564372732936No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14550.20935040942033678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13970.20100516973210344No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13290.1912210956148643No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11360.16345159113505334No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11260.16201275670604756No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10850.15611353554712398No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10770.15496246800391938No Hit
CTATAAAACTTACCCATTCACTTTCGGCCCTGGGACCAAAGTTGATCTCAAAAGAACTGTGGCTGCACCAT10300.14819994618759236No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10250.14748052897308947No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9830.14143742437126533No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9810.14114965748546418No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9640.1387036389561544No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG9340.13438713566913715No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC9310.13395548534043544No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9290.13366771845463427No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9100.13093393303952333No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8920.12834403106731299No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8690.12503471188059975No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7670.1103586007047411No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC7390.106329864303525No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA7020.10100617691620373No Hit
GTTTTATAGTGTTGGCAGTAATAAGTTGCCAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC6990.10057452658750199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT801.10649125E-860.94671670
TAGCGAC200.006261173653.90519351
GTACGTA656.499249E-848.6998669
GGTATCA13800.040.061591
GTATCAA36400.038.9254071
TGGTACG756.0995626E-736.61932467
GTGGTAT4250.034.273191
AACGCAG45550.030.8903336
TGGTATC4750.030.6588672
ATCAACG46500.030.4596823
TATCAAC47400.030.095112
TCAACGC46850.030.0908554
CAACGCA47300.029.7388975
CGGGATT450.00563320729.44979117
CGCAGAG48050.029.2310688
ACGCAGA48600.028.9579947
GGTACGT1004.2729644E-628.5379568
TTGCAAT2702.728484E-1028.37730870
AGAGTAC49550.028.28746411
CAGAGTA49700.028.13136310
Produced by FastQC (version 0.11.5)